Characterization of the plant homolog of Nijmegen breakage syndrome 1: Involvement in DNA repair and recombination

Akutsu, Noriyuki; Iijima, Kenta; Hinata, Takeo; Tauchi, Hiroshi

doi:10.1016/j.bbrc.2006.12.030

Cited by 37 publications

(42 citation statements)

References 22 publications

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“…The latter motif is also conserved in maize and is related to the TQ motif found at this position in rice and A. thaliana. The MRE11-and ATM-binding sites were found in the C-terminal region and showed high homology to those previously described in rice and A. thaliana (Akutsu et al 2007). …”

Section: Characterization Of Nbs1 Wheat Genesmentioning

confidence: 79%

“…A3). Thus, the predicted FHA and BRTC1 domains of wheat proteins perfectly match those described for rice and maize (Akutsu et al 2007;Waterworth et al 2007). A putative BRTC2 domain was also identified, based on the a/b topology recorded when using the services of the PredictProtein Server.…”

Section: Characterization Of Nbs1 Wheat Genesmentioning

confidence: 93%

“…Nbs1 is found only in eukaryotic cells (Rupnik et al 2008), and there is little homology between orthologous sequences. This lack of homology explains why Nbs1 genes have been reported only recently in A. thaliana, Oriza sativa, and maize (Akutsu et al 2007;Waterworth et al 2007).…”

Section: Introductionmentioning

confidence: 89%

“…A putative BRTC2 domain was also identified, based on the a/b topology recorded when using the services of the PredictProtein Server. Wheat NBS1 proteins showed the highly conserved plant SQ motif at position 269 (Akutsu et al 2007;Waterworth et al 2007), along with a second SQ motif at position 470. The latter motif is also conserved in maize and is related to the TQ motif found at this position in rice and A. thaliana.…”

Section: Characterization Of Nbs1 Wheat Genesmentioning

confidence: 99%

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Characterization of theNbs1Gene and Analysis of the Expression of Homologous and Homoeologous MRN Complex Genes in Meiocytes and Somatic Cells of Different Wheat Species

Pérez¹,

Cuadrado²,

Jouve³

et al. 2011

International Journal of Plant Sciences

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The products of the Nbs1, Mre11, and Rad50 genes form the MRN complex. This complex is involved mainly in DNA repair, but it also has roles in meiosis, replication, telomere maintenance, and cell cycle checkpoint signaling. Its study is therefore of great importance if our knowledge of these important cell processes is to improve. To date, few studies have examined the MRN complex in plants. The present work describes the molecular characteristics of the Nbs1 gene in diploid and polyploid species of wheat. The expression of MRN complex homologous genes in meiocytes and in somatic cells treated with genotoxic agents is also examined. In addition, the expression of MRN complex homoeologous genes is analyzed in polyploid wheat species to determine the function of the duplicated genes. The characterization of Nbs1 in wheat species revealed its low sequence homology with the Nbs1 gene of other organisms. However, the homologous Nbs1 genes of wheat showed very great similarity. All the Nbs1 wheat genes examined had the characteristic domains necessary for proper protein function. A putative second BRCT domain and a new SQ motif are here described for the first time in plants. Higher rates of Nbs1 expression were detected in meoicytes. In leaf and root cells, basal expression was observed even after treatments with genotoxic agents. Overall, Nbs1 was the MRN complex gene most weakly expressed. MRN complex homoeologous genes were expressed at different levels in polyploid species; indeed, expression was biased in some cases. No silencing of copies was detected in any of the samples analyzed, indicating maintenance of the function of MRN complex homoeologous genes.

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Section: Characterization Of Nbs1 Wheat Genesmentioning

confidence: 79%

Section: Characterization Of Nbs1 Wheat Genesmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 89%

Section: Characterization Of Nbs1 Wheat Genesmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of theNbs1Gene and Analysis of the Expression of Homologous and Homoeologous MRN Complex Genes in Meiocytes and Somatic Cells of Different Wheat Species

Pérez¹,

Cuadrado²,

Jouve³

et al. 2011

International Journal of Plant Sciences

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“…The orthologue of the third constituent of the MRN complex, Nbs1, which is known to cause human hereditary disorder Nijmegen breakage syndrome when defective, has also been found in higher plants, Arabidopsis and rice (Akutsu et al, 2007). The At NBS1 expression increased when plants were exposed to X-rays.…”

Section: Similar Results Have Been Obtained With Plants Defective Inmentioning

confidence: 96%

Cytogenetics for the study of telomere function in plants

Široký

2008

Cytogenet Genome Res

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The ends of linear chromosomes of the vast majority of eukaryotic species possess specialized nucleo-protein structures called telomeres. Regardless of many exceptions, the structure and function of telomeres share high degrees of similarity between various eukaryotes. The underlying DNA structure of telomeres determines the particular setup of telomere chromatin and protein complexes as are telomere-associated proteins and a number of repair and cell cycle checkpoint agents. The structure of telomeres is highly dynamic during the cell’s growth, replication, differentiation, senescence, or neoplastic transformation. Although the bulk of our knowledge about telomere function comes from molecular and biochemical studies in model organisms such as yeast and mouse, we want to show – with special emphasis on plants – in this short review that classical methods of plant cytogenetics can significantly complement the above experimental approaches and help in our understanding of telomere functions.

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