Characterization of the phytochelatins and their derivatives in rice exposed to cadmium based on high-performance liquid chromatography coupled with data-dependent hybrid linear ion trap orbitrap mass spectrometry

Mou, Renxiang; Cao, Zhaoyun; Lin, Xiaoyan; Wu, Li; Cao, Zhenzhen; Zhu, Zhiwei; Chen, Mingxue

doi:10.1002/rcm.7669

Cited by 6 publications

(24 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additional details of metabolite validation and PyC-metal complex collision-induced dissociation are provided in the Supplementary Information (Figure S-1 and Figure S-3 to S-8). Examples are provided along with literature data for PyCs and PyC-metal complexes in Table 2 (6,18,19,28–30).…”

Section: Resultsmentioning

confidence: 99%

“…The first PyC form identified was (γ-Glu-Cys) n -Gly (n = 2–11) (5). Other forms exist in which glycine (Gly) can be substituted with β-alanine (β-Ala), Ala, glutamine (Gln), serine (Ser) or glutamate (Glu) or with no additional amino acid (4,6–8). Because of their high thiol (-SH) content due to Cys residues, PyCs have strong metal-binding abilities with increasing metal capacity with increasing PyC size (3,9).…”

Section: Introductionmentioning

confidence: 99%

“…PyCs have been observed in a wide range of plant species (1), accumulating in different tissues depending on the plant (10). For example, in rice ( Oryza sativa ) seedlings exposed to a high dose of Cd, PyC 2 -Gly was at the highest concentration in leaves followed by roots and shoots (6). In a different study of wild basil ( Clinopodium vulgare ) grown with excess Cd, the roots had 4-fold and 10-fold higher concentrations of PyC 2 -Gly compared to leaves and shoots, respectively (11).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Phytochelatin database: a resource for phytochelatin complexes of nutritional and environmental metals

et al. 2019

View full text Add to dashboard Cite

Phytochelatins (PyCs) are a diverse set of plant compounds that chelate metals, protect against metal toxicity and function in metal homeostasis. PyCs are present in plants consumed as food by humans and could, in principle, impact absorption and utilization of essential and toxic metals such as selenium and cadmium, respectively. PyCs vary in terminal amino acid composition and chain length, exist in multiple oxidation states and reversibly bind multiple metals; consequently, PyCs include a large set of possible structures. Although individual PyC-metal complexes have been studied, no resource exists to characterize the diversity of PyCs and PyC-metal complexes. We used the scientific literature to develop a database of elemental formulas for polymer forms varying in chain length from 2 to 11 glutamyl-cysteine repeats. Using elemental formulas, we calculated monoisotopic masses using the most abundant isotopes of each element and calculated masses for complexes with 13 metals of nutritional and toxicological significance. The resulting phytochelatin database (PyCDB) contains 46 260 unique elemental formulas for PyC and PyC-metal complexes. The database is available online for download as well as for direct mass queries for mass spectrometry using an accurate mass annotation tool for user-selected PyC types, metals and adducts of interest. We performed studies of a commonly consumed food—onion—to validate the database and test utility of the tool. Onion samples were analyzed using ultra-high resolution mass spectrometry-based metabolomics. Mass spectral features were annotated using the PyCDB web tool and the R package, xMSannotator; annotated features were further validated by collision-induced dissociation mass spectrometry. The results establish use and a workflow for PyCDB as a resource for characterization of PyCs and PyC-metal complexes.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Phytochelatin database: a resource for phytochelatin complexes of nutritional and environmental metals

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Various analytical methods are used to identify PCs and their complexes with metals [38,56,74,83,120,[152][153][154], and their advantages and disadvantages are discussed in the review by Ahmad and co-authors [155]. The most numerous studies are devoted to the role of glutathione and PCs in the detoxification of Cd, since Cd ions have the highest affinity for these ligands compared to other metals (Mn, Fe, Cu, Zn) [156].…”

Section: The Structure Of Phytochelatin Complexes With Metal(loid)smentioning

confidence: 99%

“…Instead of glycine, at the C-terminus of the polypeptide there can be glutamine (Gln), glutamic acid (Glu), serine (Ser), alanine (Ala), β-alanine (β-Ala), asparagine (Asn), or cysteine (Cys), or the C-terminal amino acid may be absent. In addition, there have been found several PC derivatives that lack either Glu or Cys residues from the γ-Glu-Cys structure [45,83,86,99]. Thus, several families of PCs have been currently identified [83,110,112,165] (Table 1).…”

Section: Classification Of Phytochelatins and Their Accumulation In D...mentioning

confidence: 99%

Phytochelatins: Sulfur-Containing Metal(loid)-Chelating Ligands in Plants

Серегин

Кожевникова

2023

IJMS

View full text Add to dashboard Cite

Phytochelatins (PCs) are small cysteine-rich peptides capable of binding metal(loid)s via SH-groups. Although the biosynthesis of PCs can be induced in vivo by various metal(loid)s, PCs are mainly involved in the detoxification of cadmium and arsenic (III), as well as mercury, zinc, lead, and copper ions, which have high affinities for S-containing ligands. The present review provides a comprehensive account of the recent data on PC biosynthesis, structure, and role in metal(loid) transport and sequestration in the vacuoles of plant cells. A comparative analysis of PC accumulation in hyperaccumulator plants, which accumulate metal(loid)s in their shoots, and in the excluders, which accumulate metal(loid)s in their roots, investigates the question of whether the endogenous PC concentration determines a plant’s tolerance to metal(loid)s. Summarizing the available data, it can be concluded that PCs are not involved in metal(loid) hyperaccumulation machinery, though they play a key role in metal(loid) homeostasis. Unraveling the physiological role of metal(loid)-binding ligands is a fundamental problem of modern molecular biology, plant physiology, ionomics, and toxicology, and is important for the development of technologies used in phytoremediation, biofortification, and phytomining.

show abstract

Tentative identification of phytochelatins, their derivatives, and Cd‐phytochelatin complexes in Ocimum basilicum L. roots by Paper Spray Mass Spectrometry

Teles,

de Sousa,

Augusti

et al. 2024

J Mass Spectrom

View full text Add to dashboard Cite

An unprecedented and direct PS‐MS (paper spray ionization mass spectrometry) method was proposed for the detection of native peptides, that is, glutathiones (GSHs), homoglutathiones (hGSHs), and phytochelatins (PCs), in basil (Ocimum basilicum L.) roots before and after cadmium exposure. The roots were submitted to cold maceration followed by sonication with formic acid as the extractor solvent for sample preparation. PS‐MS was used to analyze such extracts in the positive mode, and the results allowed for the detection of several GSHs, hGSHs, and PCs. Some of these PCs were not distinguished in the control samples, that is, basil roots not exposed to cadmium. Other PCs were noticed in both types of roots, uncontaminated and cadmium‐contaminated, but the intensities were higher in the former samples. Moreover, long‐time exposure to cadmium stimulated the formation of some of these PCs and their cadmium complexes. The results, therefore, provided some crucial insights into the defense mechanism of plants against an external stress condition due to exposure to a toxic heavy metal. The present study represents a promising alternative to investigate other crucial physiological processes in plants submitted to assorted stress conditions.

show abstract

Characterization of the phytochelatins and their derivatives in rice exposed to cadmium based on high-performance liquid chromatography coupled with data-dependent hybrid linear ion trap orbitrap mass spectrometry

Cited by 6 publications

References 32 publications

Phytochelatin database: a resource for phytochelatin complexes of nutritional and environmental metals

Phytochelatin database: a resource for phytochelatin complexes of nutritional and environmental metals

Phytochelatins: Sulfur-Containing Metal(loid)-Chelating Ligands in Plants

Tentative identification of phytochelatins, their derivatives, and Cd‐phytochelatin complexes in Ocimum basilicum L. roots by Paper Spray Mass Spectrometry

Contact Info

Product

Resources

About