Characterization of the photoinduced electron transfer reaction from the photosynthetic system in Rhodobacter sphaeroides to an exogenous electron acceptor

Kasuno, Megumi; Takasu, Masaki; Tsukatani, Yusuke; Murakami, Daisuke; Hanada, Satoshi; Matsushita, Takayuki; Tao, Hiroaki

doi:10.1016/j.jelechem.2009.09.017

Cited by 17 publications

(28 citation statements)

References 28 publications

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“…By monitoring decreases or increases in I0 and Ic, qualitative and quantitative analyses are possible. In addition, given that the photocurrent of chromatophore vesicles is more stable than that of live cells [3], the system described herein represents an attractive technology for sensing harmful compounds in wastewater effluents.…”

Section: Discussionmentioning

confidence: 99%

“…Bacterial cultivation and chromatophore vesicle preparation followed previously described procedures [3]. The amount of R. sphaeroides chromatophores corresponding to a concentration of bacteriochlorophyll (BChl) a of 5 M was adopted in the present work.…”

Section: Preparation Of Chromatophore Vesicles and Reagentsmentioning

confidence: 99%

“…In addition, photosynthetic membranes (so-called chromatophores, which are inside-out bimolecular lipid membrane vesicles) can be readily prepared from R. sphaeroides. We previously developed a system using a rotating-disk electrode for the measurement of photo-induced electron transfer from chromatophores of R. sphaeroides through an exogenous mediator [3]. Though rotating-disk electrodes are useful for kinetic investigations of photo-induced electron transfer, carbon paste electrodes (CPEs) are preferable for detecting toxic substances, given that the electrodes can be modified with chromatophore vesicles [4].…”

Section: Introductionmentioning

confidence: 99%

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<strong>Evaluation of sensor performance for harmful compounds by using photo-induced electron transfer from photosynthetic membranes to electrodes</strong>

Kasuno

Kimura

Yasutomo

et al. 2015

Proceedings of 2nd International Electronic Conference on Sensors and Applications

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Abstract:The effect of harmful compounds such as KCN, phenol, and herbicides on the photocurrent of photosynthetic membranes (so-called chromatophores) was investigated using carbon paste electrodes. The photocurrent-time curve of photo-induced electron transfer from chromatophores of the purple photosynthetic bacterium Rhodobacter sphaeroides to the electrode via 2,5-dichloro-1,4-benzoquinone (DCBQ) was composed of two characteristic phases: an abrupt increase in current immediately after illumination (I0), and constant current over time (Ic). Photo-reduction of DCBQ exhibited Michaelis- OPEN ACCESS 2Menten-like kinetics, and reduction rates were dependent on the amount of DCBQ and the photon flux intensity. The Ic decreased in the presence of KCN at concentrations over 0.05). The I0 decreased selectively following addition of phenol at concentrations over 20 μM. The Ic was affected by terbutryn only at concentrations over 10 μM. In contrast, DCMU and atrazine had no effect on either I0 or Ic. The utility of this electrode system for the detection of harmful compounds is also discussed.

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Section: Discussionmentioning

confidence: 99%

Section: Preparation Of Chromatophore Vesicles and Reagentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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<strong>Evaluation of sensor performance for harmful compounds by using photo-induced electron transfer from photosynthetic membranes to electrodes</strong>

Kasuno

Kimura

Yasutomo

et al. 2015

Proceedings of 2nd International Electronic Conference on Sensors and Applications

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“…Iron reduction rates are typically quantified colourimetrically [24][25][26], but can also be measured using liquid scintillation analysis [1, 16], or electrochemically [27,28]. Electrochemical measurements are carried out by placing an electrode in cell suspensions and measuring the current due to oxidation of any reduced mediator in solution [27,28].…”

Section: Introductionmentioning

confidence: 99%

“…Electrochemical measurements are carried out by placing an electrode in cell suspensions and measuring the current due to oxidation of any reduced mediator in solution [27,28]. This was the approach taken in a previous paper by the authors to measure the reduction of ferricyanide by the algal species C. vulgaris [29].…”

Section: Introductionmentioning

confidence: 99%

Iron reduction by the cyanobacterium Synechocystis sp. PCC 6803

Thorne

Schneider

et al. 2015

Bioelectrochemistry

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Synechocystis sp. PCC 6803 uptakes iron using a reductive mechanism, similar to that exhibited by many other microalgae. Various bio-electrochemical technologies have made use of this reductive cellular capacity, but there is still a lack of fundamental understanding of cellular reduction rates under different conditions. This study used electrochemical techniques to further investigate the reductive interactions of Synechocystis cells with Fe(III) from the iron species potassium ferricyanide, with varying cell and ferricyanide concentrations present. At the lowest cell concentrations tested, cell reduction machinery appeared to kinetically limit the reduction reaction, but ferricyanide reduction rates were mass transport controlled at the higher cell and ferricyanide concentrations studied. Improving the understanding of the reduction of Fe(III) by whole cyanobacterial cells is important for improving the efficiencies of technologies that rely on this interaction.

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Specific Interaction between Redox Phospholipid Polymers and Plastoquinone in Photosynthetic Electron Transport Chain

et al. 2017

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Redox phospholipid polymers added in culture media are known to be capable of extracting electrons from living photosynthetic cells across bacterial cell membranes with high cytocompatibility. In the present study, we identify the intracellular redox species that transfers electrons to the polymers. The open-circuit electrochemical potential of an electrolyte containing the redox polymer and extracted thylakoid membranes shift to positive (or negative) under light irradiation, when an electron transport inhibitor specific to plastoquinone is added upstream (or downstream) in the photosynthetic electron transport chain. The same trend is also observed for a medium containing living photosynthetic cells of Synechococcus elongatus PCC7942. These results clearly indicate that the phospholipid redox polymers extract photosynthetic electrons mainly from plastoquinone.

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Characterization of the photoinduced electron transfer reaction from the photosynthetic system in Rhodobacter sphaeroides to an exogenous electron acceptor

Cited by 17 publications

References 28 publications

<strong>Evaluation of sensor performance for harmful compounds by using photo-induced electron transfer from photosynthetic membranes to electrodes</strong>

<strong>Evaluation of sensor performance for harmful compounds by using photo-induced electron transfer from photosynthetic membranes to electrodes</strong>

Iron reduction by the cyanobacterium Synechocystis sp. PCC 6803

Specific Interaction between Redox Phospholipid Polymers and Plastoquinone in Photosynthetic Electron Transport Chain

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