Characterization of the Penetration of the Blood–Brain Barrier by High-Performance Liquid Chromatography (HPLC) Using a Stationary Phase with an Immobilized Artificial Membrane

Doležal, Rafael; Karásková, Natálie; Musil, Karel; Novák, Miroslav; Maltsevskaya, Nadezhda V.; Maliňák, Dávid; Kolář, Karel; Soukup, Ondřej; Kuča, Kamil; Karasová, Jana Žďárová

doi:10.1080/00032719.2018.1424175

Cited by 8 publications

(1 citation statement)

References 16 publications

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“…Furthermore, there is an increasing tendency towards studying the composition of small molecules, such as metabolites, due to the fact that their levels can be significantly influenced by many diseases, the administration of drugs, or by toxins [12,13]. Thus, blood measurements of metabolites have a wide range of applications [14] using various technologies, such as proton nuclear magnetic resonance spectroscopy ( 1 H-NMR) [15], mass spectroscopy (MS) [16] and high-performance liquid chromatography (HPLC) [17], etc. Among these methods, 1 H-NMR is the most often utilized to provide chemical and structural information of biological molecules [18] in a sample without any damage.…”

Section: Introductionmentioning

confidence: 99%

NMR Based Metabolomics Comparison of Different Blood Sampling Techniques in Awake and Anesthetized Rats

Zhang

et al. 2019

Molecules

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The composition of body fluids has become one of the most commonly used methods for diagnosing various diseases or monitoring the drug responses, especially in serum/plasma. It is therefore vital for investigators to find an appropriate way to collect blood samples from laboratory animals. This study compared blood samples collected from different sites using the NMR based metabolomics approach. Blood samples were collected from the saphenous vein (awake state), tail vein (awake and anesthetized states after administration of sevoflurane or pentobarbital) and the inferior thoracic vena cava (ITVC, anesthetized state). These approaches from the saphenous and tail veins have the potential to enable the collection of multiple samples, and the approach from ITVC is the best method for the collection of blood for the terminate state. The compositions of small molecules in the serum were determined using the 1H-NMR method, and the data were analyzed with traditional correlation analysis, principle component analysis (PCA) and OPLS-DA methods. The results showed that acute anesthesia significantly influenced the composition of serum in a very short period, such as the significant increase in glucose, and decrease in lactate. This indicates that it is better to obtain blood samples under the awake state. From the perspective of animal welfare and multiple sampling, the current study shows that the saphenous vein and tail vein are the best locations to collect multiple blood samples for a reduced risk of injury in the awake state. Furthermore, it is also suitable for investigating pharmacokinetics and the effects of drug intervention on animals.

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