Characterization of the Pattern of Amino Acid Incorporation in Cell-Free Liver Systems by Autoradiography of Precipitin Reactions

Abstract

It was shown in preceding papers (13,18) that the pattern of protein labeling in amino acid incorporation experiments can be studied in certain detail by combining immunological reaclions on agar plates with autoradiography. In those experiments the labeling of different protein antigens was studied after incubation of liver slices with 14C amino acids under varied experimental conditions. The present communication aims at a similar analysis of the labeling of proteins in cell-free liver systems.

Labeling With 14c Amino Acids of Albumin-Like Protein by Rat Liver Ribonucleoprotein Particles

“…It has been shown in a number of publications (23,24,(27)(28)(29) that the pattern of protein labeling in an amino acid-incorporating experiment can be studied by combining immunological reactions in agar gel with autoradiography. Most of these reports concern incorporating experiments with the 12,000 g supernatant of a rat liver homogenate or a microsomal-cell sap system.…”

“…For this purpose, advantage has been taken of a method worked out by Perlmann et al (23,24,(27)(28)(29). This technique combines immunoelectropboresis in agar gel with autoradiography.…”