Characterization of the partial VP2 gene region of canine parvoviruses in domestic cats from Turkey

Muz, Dilek; Oğuzoğlu, Tuba Çiğdem; Timurkan, Mehmet Ozkan; Akin, Humay

doi:10.1007/s11262-011-0703-8

Cited by 18 publications

(33 citation statements)

References 37 publications

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“…After trimming and editing processes of the low-quality nucleotides in relevant sequences, nucleotide similarity searches by the Basic Local Alignment Search Tool (BLAST) revealed that the sequences of the blood and rectal swab samples were 98.42-99.47% identical to various CPV and FPV strains. Phylogeographic analysis of the 325 bp nucleotide sequence of the field virus with several canine and feline parvovirus sequences revealed that the obtained sequences were located inside the canine parvovirus strains with an FPV strain (HM042740.1) isolated from a cat and reported by Muz et al (22) (Fig. 1).…”

Section: Resultsmentioning

confidence: 64%

“…After mutations, the CPV-2 variants that have a high rate of evolution compared to FPV have become able to infect both dogs and cats and have been found in wolves, foxes, other canids and felids (14,15,32). CVP-2 has been previously reported in cats and dogs in Turkey, so obtaining up to date information representing the circulation of CPV variants is a necessity (22,28,35,39). The objective of this study was to determine the CPV variants in pups with clinical symptoms and to demonstrate its relationship with the other types reported in Turkey and around the world.…”

Section: Praca Oryginalnamentioning

confidence: 99%

“…The CPV type 2 is no longer detectable in the field and the type 2a, 2b and 2c variants have circulated instead. Notifications were made from Turkey regarding the CPV infections and although there are submissions of the 2c strains to the gene bank from Turkey, most circulating CPVs in dogs are the 2a and 2b strains (22,35,39). In this context, phylogenetic studies are needed to better understand the evolutionary relationships in the country in order to characterise the actual strains and elucidate the current situation.…”

Section: Sdt Color-coded Matrix Of Pairwise Identity Scores Generatmentioning

confidence: 99%

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Phylogenetic analysis of canine parvoviruses from Turkey

Akkutay-Yoldar¹,

Koç²

2020

Medycyna Weterynaryjna

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Canine parvovirus (CPV) type 2 is the causative agent of acute hemorrhagic enteritis and high mortality in the affected dogs. Numerous studies have been done to understand the origin of the virus and to exhibit new variants and circulating strains. This report describes the detection and genomic characterization of CPV strains from indoor and outdoor dogs in Ankara, Turkey. Samples were sent to our laboratory due to clinical symptoms in puppies. We tested blood and swab samples to determine the presence of canine parvovirus (CPV) in three puppies and two adult dogs by reverse transcription-polymerase chain reaction (RT-PCR) using VP2 (capsid protein) region primers of canine parvoviruses. Following that, to provide molecular characterization data Maximum Likelihood (ML) method was used for phylogenetic analyses. Constructed phylogenetic trees from the aligned nucleotide sequences revealed that our CPV strains demonstrated high genetic similarities, with 100% identity match on nucleotide alignments with each other and classified in CPV-2b genotypes.They have placed on a monophyletic clade as a sister branch with CPV VAC S quantum with 98.9% nucleotide homology. Our findings suggest that CPV-2b is actual and frequently seen variant in Turkey and shows high similarities with other CPV variants and a bit less with FPVs in Turkey and around the world. CPV causes high mortality and morbidity in dogs and to develop effective vaccines for protection of dogs in Turkey where there are few numbers of studies that have been done, field strains should be isolated and characterised.

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Section: Resultsmentioning

confidence: 64%

Section: Praca Oryginalnamentioning

confidence: 99%

Section: Sdt Color-coded Matrix Of Pairwise Identity Scores Generatmentioning

confidence: 99%

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Phylogenetic analysis of canine parvoviruses from Turkey

Akkutay-Yoldar¹,

Koç²

2020

Medycyna Weterynaryjna

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“…However, the possibility of the presence of CPV-2 strains cannot be ruled out, considering the report of isolating CPV-2 strains from cats in the neighboring country "Turkey" (Muz et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…The original CPV-2 was rapidly replaced with a new antigenic type CPV-2a, CPV-2b in 1979-1984(Parrish et al, 1991 and finally, the newest strain of CPV-2c was detected in 2000 in Italy (Buonavoglia et al, 2001). In contrast with the original CPV-2; CPV-2a/2b/2c earned the ability to replicate in a cat host and could also induce the clinical disease (Mochizuki et al, 1993;Truyen et al, 1996;Ikeda et al, 2000;Battilani et al, 2006a;Muz et al, 2012;Decaro et al, 2008;2011). CPV-2 variants (2a, 2b, 2c) are able to bind with feline Transferrin receptors and enter into the cells (Ikeda et al, 2002;Hueffer and Parrish, 2003) Despite CPV, FPV is evolutionarily static and develops by random genetic drift (Decaro et al, 2008) whereas the mutation rate of CPV is much higher.…”

Section: Introductionmentioning

confidence: 99%

Sequence Analysis of VP2 Gene of the Parvoviruses Isolated from Fecal Samples of Domestic Cats in Tehran, Iran

Jahanbin

Jamshidi

Madadgar

2017

American Journal of Animal and Veterinary Sciences

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Panleukopenia is a viral enteritis of high mortality rate in Feline population. It is caused by a small single-stranded DNA virus, belonging to the Family Parvoviridae, called Feline Panleukopenia Virus (FPV). Parvoviruses are capable of adapting to new hosts and can simply mutate like RNA viruses, resulting in the emergence of different strains with antigenic changes. Unlike the original Canine Parvovirus (CPV) type 2, the antigenic variants (CPV-2a, CPV-2b, CPV-2c) earned the ability to replicate in cat hosts either with clinical signs or without any symptoms. Cats may be able to shed Parvoviruses for a long time and can serve as an important reservoir. Co-infection with FPV and CPV-2 variants has been also reported. These characteristics make the cat an important host for Parvoviruses. Since no molecular study has reported on Parvoviruses infected cats in Iran, a decision was made to study these strains of Parvovirus. Twenty-eight fecal samples were collected from kittens under 6 months of age, both male and female and different breeds which had leukemia. PCR was performed on each sample and 26 samples tested positive for Parvoviruses. Randomly, the PCR products of eight samples were sequenced and their phylogenetic tree was generated. Analysis of the results revealed that all eight samples are FPV and it appears that FPV is more clonal than the CPV-2 variants in Iran. Amino acid residue 232 from the VP2 gene of FPVs has been changed from isoleucine to valine, which has only been reported in CPV-2 and its variants. It seems that some FPVs of Iran are changing to the CPV-2 variants. Hence, it is expected that in the future, the CPV-2 variants will be observed in cats.

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