Characterization of the oral microbiota of healthy cats using next-generation sequencing

Sturgeon, Amy; Pinder, Shaun; Costa, Márcio Paulino; Weese, J. Scott

doi:10.1016/j.tvjl.2014.01.024

Cited by 70 publications

(95 citation statements)

References 23 publications

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“…Dolieslager et al (2011) investigated the oral microbiota of three healthy cats and five cats with feline chronic gingivostomatitis, and Neisseria was only found in healthy cats. A more comprehensive NGS-based approach has characterized the oral microbiota of 11 healthy cats, and once more, Neisseria was a predominant taxon and part of the core microbiome (Sturgeon et al, 2014).…”

Section: Identification Of Neisseria Species In Non-human Mammalian Hmentioning

confidence: 99%

Non-pathogenic Neisseria: members of an abundant, multi-habitat, diverse genus

2015

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Section: Identification Of Neisseria Species In Non-human Mammalian Hmentioning

confidence: 99%

Non-pathogenic Neisseria: members of an abundant, multi-habitat, diverse genus

2015

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“…However culture-based studies, while useful to enable precise characterisation of putative periodontopathogens, generally underestimate microbial community diversity. Culture-independent methods, such as high-throughput DNA sequencing reveal a rich and diverse bacterial community in the oral cavities of humans and companion animals [6][7][8] , and the marsupial microbiome should also be more thoroughly studied too.…”

mentioning

confidence: 99%

“…More recently, researchers have used highthroughput DNA sequencing to study the oral microbiota of healthy cats 8 and dogs 7 . In adult horses 67% of 203 operational taxonomic units (OTUs) were recovered, with the most frequent genera being Prevotella and Porphyromonas (T Chinkangsadarn, GJ Wilson, SW Corley and PS Bird, 2014, unpublished).…”

mentioning

confidence: 99%

Marsupial oral cavity microbiome

et al. 2015

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“…This new molecular approach has been applied in dogs (Sturgeon et al 2013) and cats (Sturgeon et al 2014) to investigate oral bacterial communities and periodontal plaque ecology with minimal time and cost.…”

Section: Bacterial Culture and Identification;mentioning

confidence: 99%

“…Molecular methods have played a major role in the identification of bacterial isolates from both human and veterinary clinical specimens (Clarridge 2004;Spratt 2004;Kato et al 2011;Riggio et al 2011). Further technological advances, including next generation sequencing (NGS), have provided even more detailed information about the oral microbiome in humans (Keijser et al 2008) and animals, such dogs (Sturgeon et al 2013) and cats (Sturgeon et al 2014). …”

Section: Introductionmentioning

confidence: 99%

Clinical and microbiological aspects of periodontal disease in horses in South-East Queensland, Australia

Chinkangsadarn¹

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The study of periodontal disease as part of equine dentistry is one of the overlooked fields of study, which truly needs more study and research to clearly understand the nature of the disease, the most appropriate diagnostic technique and prevention or treatment to provide for a good quality of life for horses.The abattoir survey of the oral cavity and dentition of 400 horses from SouthEast Queensland, Australia, showed that the most common dental abnormality was sharp enamel points (55.3% prevalence). Several types of dental abnormalities were strongly associated with age. The highest frequency of dental abnormalities (97.5%) were observed in senior horses (11-15 years old) and this included periodontal disease that increased to almost fifty percent in senior horses. The findings also confirmed that all horses, not just young horses, should have regular complete dental examinations as early as possible which should limit the development of more severe dental pathologies later in life.The equine oral microbiome found in dental plaque can cause oral disease which involves the some of the endogenous oral microbiota becoming opportunistic pathogens. The conventional method of oral microbiology based on culture dependent techniques usually overestimates the significance of species that are easily grown and overlooks microbial community diversity. Recently, the culture independent techniques using the next generation sequencing (NGS) method can determine the whole bacterial microbiota. The results from culture dependent and NGS method of analysis of healthy gingiva and periodontitis samples showed that the most common Genera isolated were Prevotella and Porphyromonas without a statistical significance in bacterial diversity, between the control and periodontitis groups. In conclusion, equine bacterial diversity between the healthy and periodontitis groups were similar which is different from other animals and this may be due to factors such as dentition, feed type and husbandry.The standard approach for diagnosis of equine periodontal disease (EPD) is based on clinical dental examination but to evaluate the current stage of disease and for further treatment planning, auxiliary aids such radiography are required. Equine dental radiography has been largely restricted to extraoral techniques by the standard equine radiography technology and the complexity of equine head anatomy. In III addition, superimposition and distortion of the cheek teeth arcade is a major problem for radiographic interpretation. Intraoral radiography has been largely ignored in horses, due to the uncooperative nature of these patients and the absence of dedicated technology unsuitable for equine oral radiography. In this study, radiographic images from seven horse heads obtained using a commercially available equine intraoral computed radiography system (IO-CR) and 3D imaging from computed tomography file sets were reviewed. IO-CR has shown potential as a diagnostic tool where key pathological lesions of EPD of the cheek teeth were demon...

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Characterization of the oral microbiota of healthy cats using next-generation sequencing

Cited by 70 publications

References 23 publications

Non-pathogenic Neisseria: members of an abundant, multi-habitat, diverse genus

Non-pathogenic Neisseria: members of an abundant, multi-habitat, diverse genus

Marsupial oral cavity microbiome

Clinical and microbiological aspects of periodontal disease in horses in South-East Queensland, Australia

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