Characterization of the olfactory system of the giant honey bee, Apis dorsata

Mogily, Sandhya; Vijaykumar, Meenakshi; Sethy, Sunil Kumar; Joseph, Jeymohan

doi:10.1007/s00441-019-03078-8

Cited by 2 publications

(2 citation statements)

References 64 publications

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“…As manually curated OR gene regions were unavailable, we simply compared the insectOR prediction with those available at NCBI (Table 3). The expected number of ORs in A. dorsata is > = 165 given the discovery of~165 olfactory glomeruli [54] as well as given the similar number of ORs in the other honey bees [16,19].…”

Section: Plos Onementioning

confidence: 97%

InsectOR—Webserver for sensitive identification of insect olfactory receptor genes from non-model genomes

Karpe

Tiwari²,

Sowdhamini³

2021

PLoS ONE

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Insect Olfactory Receptors (ORs) are diverse family of membrane protein receptors responsible for most of the insect olfactory perception and communication, and hence they are of utmost importance for developing repellents or pesticides. Accurate gene prediction of insect ORs from newly sequenced genomes is an important but challenging task. We have developed a dedicated webserver, ‘insectOR’, to predict and validate insect OR genes using multiple gene prediction algorithms, accompanied by relevant validations. It is possible to employ this server nearly automatically and perform rapid prediction of the OR gene loci from thousands of OR-protein-to-genome alignments, resolve gene boundaries for tandem OR genes and refine them further to provide more complete OR gene models. InsectOR outperformed the popular genome annotation pipelines (MAKER and NCBI eukaryotic genome annotation) in terms of overall sensitivity at base, exon and locus level, when tested on two distantly related insect genomes. It displayed more than 95% nucleotide level precision in both tests. Finally, given the same input data and parameters, InsectOR missed less than 2% gene loci, in contrast to 55% loci missed by MAKER for Drosophila melanogaster. The webserver is freely available on the web at http://caps.ncbs.res.in/insectOR/ and the basic package can be downloaded from https://github.com/sdk15/insectOR for local use. This tool will allow biologists to perform quick preliminary identification of insect olfactory receptor genes from newly sequenced genomes and also assist in their further detailed annotation. Its usage can also be extended to other divergent gene families.

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Section: Plos Onementioning

confidence: 97%

InsectOR—Webserver for sensitive identification of insect olfactory receptor genes from non-model genomes

Karpe

Tiwari²,

Sowdhamini³

2021

PLoS ONE

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“…The polyclonal antiserum against GABA was produced in rabbit with GABA–BSA as an immunogen. It has a wide range with positive staining in many insects, for example Drosophila (Seki et al., 2010), Apis (Ai et al., 2017; Mogily et al., 2020), Nymphalidae (Carlsson et al., 2013), and Hemissenda (Webber et al., 2017). According to the manufacturer, the antiserum showed positive binding with GABA but not with BSA in a dot blot assay.…”

Section: Methodsmentioning

confidence: 99%

Neurochemical diversity in the central olfactory pathway of the crustacean Parhyale hawaiensis (Amphipoda): evolutionary implications

Kümmerlen

Raspe

Harzsch

2023

J of Comparative Neurology

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In mandibulate arthropods, the primary olfactory centers, termed olfactory lobes in crustaceans, are typically organized in distinct fields of dense synaptic neuropils called olfactory glomeruli. In addition to olfactory sensory neuron terminals and their postsynaptic efferents, the glomeruli are innervated by diverse neurochemically distinctive interneurons. The functional morphology of the olfactory glomeruli is understudied in crustaceans compared with insects and even less well understood and described in a particular crustacean subgroup, the Peracarida, which embrace, for example, Amphipoda and Isopoda. Using immunohistochemistry combined with confocal laser scanning microscopy, we analyzed the neurochemistry of the olfactory pathway in the amphipod Parhyale hawaiensis. We localized the biogenic amines serotonin and histamine as well as the neuropeptides RFamide, allatostatin, orcokinin, and SIFamide.As for other classical neurotransmitters, we stained for γ-aminobutyric acid and glutamate decarboxylase and used choline acetyltransferase as indicator for acetylcholine.Our study is another step in understanding principles of olfactory processing in crustaceans and can serve as a basis for understanding evolutionary transformations of crustacean olfactory systems.

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Characterization of the olfactory system of the giant honey bee, Apis dorsata

Cited by 2 publications

References 64 publications

InsectOR—Webserver for sensitive identification of insect olfactory receptor genes from non-model genomes

InsectOR—Webserver for sensitive identification of insect olfactory receptor genes from non-model genomes

Neurochemical diversity in the central olfactory pathway of the crustacean Parhyale hawaiensis (Amphipoda): evolutionary implications

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