Characterization of the nucleocapsid protein of Hantaan virus strain 76-118 using monoclonal antibodies

Yoshimatsu, Kumiko; Arikawa, Jiro; Tamura, Manabu; Yoshida, Reiko; Lundkvist, Åke; Niklasson, Bo; Kariwa, Hiroaki; Azuma, Ichiro

doi:10.1099/0022-1317-77-4-695

Cited by 83 publications

(94 citation statements)

References 31 publications

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“…Unfortunately, in some patients (25/71), we could not differentiate the infecting hantavirus. Serotype-specific conformational epitopes have been detected in the center and C-terminal regions of the N. 24,36 Thus, a serotyping system using N-terminal truncated N could be a solution for the differential diagnosis of hantavirus infections. 37,38 In conclusion, new isolates of AMRV and HTNV were characterized, and AMRV, HTNV, and SEOV infections were differentiated in HFRS patients of Far East Russia.…”

Section: Discussionmentioning

confidence: 99%

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Isolation and Characterization of Hantaviruses in Far East Russia and Etiology of Hemorrhagic Fever with Renal Syndrome in the Region

Kariwa¹,

Yoshikawa²,

Tanikawa³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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Abstract. Hemorrhagic fever with renal syndrome (HFRS) is a serious public health issue in Far East Russia. Two different hantaviruses were isolated from rodents captured in the Khabarovsk region: Amur virus (AMRV; Khekhtsir/ AP209/2005 strain from Apodemus peninsulae) and Hantaan virus (HTNV; Galkino/AA57/2002 strain from A. agrarius). Genetic analysis of the new isolates revealed that the M and L segments were apparently different between AMRV and HTNV, but S segments of the two viruses were closer. The antigenicities of AMRV, HTNV, and Seoul virus (SEOV) were differentiated by cross-neutralization. Serological differential diagnoses of 67 HFRS patients in the Prymorsky and Khabarovsk regions of Far East Russia were conducted using a neutralization test. The results revealed that the major cause of HFRS varied with location in Far East Russia: SEOV for Vladivostok city in the Prymorsky region, AMRV in rural areas of the Primorsky region, and probably HTNV for the Khabarovsk region.

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Section: Discussionmentioning

confidence: 99%

“…For the detection of hantaviral antigens in Vero E6 cells, the inoculated cells on 24-well slides were visualized using a monoclonal antibody E5/G6 to hantavirus N 24 and Alexa Fluor 488 goat anti-mouse IgG.…”

Section: Methodsmentioning

confidence: 99%

Isolation and Characterization of Hantaviruses in Far East Russia and Etiology of Hemorrhagic Fever with Renal Syndrome in the Region

Kariwa¹,

Yoshikawa²,

Tanikawa³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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“…Briefly, 96-well plates were coated with the monoclonal antibody (MAb) E5/G6 [26,47] as a capture antibody and were treated with Block Ace (Dainippon Sumitomo Pharma Co., Ltd., Osaka, Japan) at 37C for 1 hr for blocking. The Baculovirus-expressed recombinant hantavirus NP of PUUV, DOBV, or HTNV [2,20] was then added to the plates and incubated at room temperature for 1 hr.…”

Section: Methodsmentioning

confidence: 99%

Epidemiological Study of Hantavirus Infection in the Samara Region of European Russia

et al. 2009

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ABSTRACT. European Russia is a highly endemic area of hemorrhagic fever with renal syndrome (HFRS), a rodent-borne zoonotic disease, caused by hantaviruses. In total, 145 small mammals of four species (Myodes glareolus, Apodemus flavicollis, A. agrarius, and A. uralensis) were trapped in the Samara region of European Russia in August 2005 and examined for the presence of hantavirus (HV). Anti-HV antibodies were found in six of 68 (8.8%) M. glareolus and in one of 19 (5.3%) A. flavicollis by indirect immunofluorescent antibody assay (IFA). The Puumala virus (PUUV), which is one of the hantavirus species, was detected in the lungs of seven M. glareolus by RT-PCR. The virus S-segment was extremely similar (96.2% to 99.3%) to the sequence found in a fatal case of HFRS in the Samara region. Phylogenetic analyses of S and M segments showed that the Samara PUUVs form a cluster within the Russian Volga lineage and apparently differ from other European PUUVs. Anti-PUUV antibodies were found in blood sera from seven HFRS patients and from one undiagnosed patient from the Samara region, using IFA and an enzyme-linked immunosorbent assay (ELISA). These data suggest that the bank vole M. glareolus is a primary natural reservoir and vector for PUUV, which is the main causative agent of HFRS in humans in the Samara region.KEY WORDS: epidemiology, hantavirus, hemorrhagic fever with renal syndrome, Puumala virus, rodents.J. Vet. Med. Sci. 71(12): 1569-1578, 2009 Hantaviruses belong to the genus Hantavirus, within the family Bunyaviridae, and cause two forms of human illness. These zoonoses include hemorrhagic fever with renal syndrome (HFRS), which occurs in Asia and Europe and is caused by the Hantaan virus (HTNV), Seoul virus (SEOV), Puumala virus (PUUV), Dobrava-Belgrade virus (DOBV), and Amur virus (AMRV). The second form of illness is hantavirus (cardio) pulmonary syndrome (HPS), which occurs in the Americas and is caused by the Sin Nombre virus, Andes virus, and other hantaviruses [13,28,33]. Hantaviruses are transmitted by aerosolized excreta from their natural hosts, mainly rodents of the family Muridae. Particular hantavirus species are usually harbored by a single rodent species, or by a few closely related rodent species, which indicates co-evolution and co-speciation of hantaviruses with their hosts. The virus genome contains three segments of negative-stranded RNA: the large (L) segment encodes a viral RNA-dependent RNA polymerase, the medium (M) segment encodes a glycoprotein precursor, and the small (S) segment encodes a nucleocapsid protein (NP) [34].In

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“…A serological test using recombinant antigens has been reported for many pathogens [5,11,12,[15][16][17][18]. If a recombinant viral protein is used as an antigen for ELISA, the risk in the processing of antigen preparations can be eliminated and many high quality antigens can be produced.…”

Section: Introductionmentioning

confidence: 99%

Establishment of an Enzyme-Linked Immunosorbent Assay for Detection of Hantavirus Antibody of Rats Using a Recombinant of Nucleocapsid Protein Expressed in Escherichia coli.

et al. 2003

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Abstract:A recombinant nucleocapsid protein of Hantaan virus (HTN) 76-118 strain expressed in E. coli was applied as a serodiagnostic antigen in an enzyme-linked immunosorbent assay (rHTN-ELISA) for detection of hantavirus antibody in rat sera. The sensitivity and specificity of the rHTN-ELISA were compared with those of the indirect immunofluoresent assay (IFA) using virus-infected cells. The sensitivity of rHTN-ELISA was similar to that of the IFA both in experimentally SR-11 infected rat and naturally infected rat sera. Sera showing a low antibody titer in IFA and suspected to be negative by other methods were also found to be negative in rHTN-ELISA. These results indicate that rHTN-ELISA is effective as a screening method for serodiagnosis of hantaviruses, because of its high sensitivity, specificity, safety and suitability for processing large number of samples.

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Characterization of the nucleocapsid protein of Hantaan virus strain 76-118 using monoclonal antibodies

Cited by 83 publications

References 31 publications

Isolation and Characterization of Hantaviruses in Far East Russia and Etiology of Hemorrhagic Fever with Renal Syndrome in the Region

Isolation and Characterization of Hantaviruses in Far East Russia and Etiology of Hemorrhagic Fever with Renal Syndrome in the Region

Epidemiological Study of Hantavirus Infection in the Samara Region of European Russia

Establishment of an Enzyme-Linked Immunosorbent Assay for Detection of Hantavirus Antibody of Rats Using a Recombinant of Nucleocapsid Protein Expressed in Escherichia coli.

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