Characterization of the non-glandular gastric region microbiota in Helicobacter suis-infected versus non-infected pigs identifies a potential role for Fusobacterium gastrosuis in gastric ulceration

Abstract

Helicobacter suis has been associated with development of gastric ulcers in the non-glandular part of the porcine stomach, possibly by affecting gastric acid secretion and altering the gastric microbiota. Fusobacterium gastrosuis is highly abundant in the gastric microbiota of H. suis -infected pigs and it was hypothesized that this micro-organism could play a role in the development of gastric ulceration. The aim of this study was to obtain further insights in the influence of a naturally acquired H. suis infection on the microbiota of the non-glandular part of the porcine stomach and in the pathogenic potential of F. gastrosuis . Infection with H. suis influenced the relative abundance of several taxa at phylum, family, genus and species level. H. suis -infected pigs showed a significantly higher colonization rate of F. gastrosuis in the non-glandular gastric region compared to non-infected pigs. In vitro, viable F. gastrosuis strains as well as their lysate induced death of both gastric and oesophageal epithelial cell lines. These gastric cell death inducing bacterial components were heat-labile. Genomic analysis revealed that genes are present in the F. gastrosuis genome with sequence similarity to genes described in other Fusobacterium spp. that encode factors involved in adhesion, invasion and induction of cell death as well as in immune evasion. We hypothesize that, in a gastric environment altered by H. suis , colonization and invasion of the non-glandular porcine stomach region and production of epithelial cell death inducing metabolites by F. gastrosuis , play a role in gastric ulceration. Electronic supplementary material The online version of this article (10.1186/s13567-019-0656-9) contains supplementary material, which is available to authorized users.

Infection Heterogeneity and Microbiota Differences in Chicks Infected by Salmonella enteritidis

“…Among them, Fusobacterium , Helicobacter , and Butycicoccus were identified as marker microorganisms in group S using LEfSe. As we know, Fusobacterium has been associated with gastric ulcers in pigs [ 49 ] and colon carcinoma in humans [ 50 , 51 ], and may represent a kind of new opportunistic pathogens of chickens worthy of further investigation [ 52 ]. In addition, in the species level, Helicobacter_pullorum has also been identified as a marker microorganism of group S, which is member of Campilobacterota and a well-known zoonotic pathogen [ 53 ].…”

Gastric Ulceration and Immune Suppression in Weaned Piglets Associated with Feed-Borne Bacillus cereus and Aspergillus fumigatus

“…More recently, Fusobacterium gastrosuis (F. gastrosuis) is identified based on 16S rRNA and gyrase B genes, and it was hypothesized that this micro-organism could play a role in the development of gastric ulceration because most Fusobacterium spp can aggravate necrosis [22]. H. suis-infected pigs showed a significantly higher colonization rate of F. gastrosuis in the non-glandular gastric region compared to non-infected pigs [23]. A previous study has demonstrated an association between gastric lesions and a carbohydrate-enriched liquid diet when mono-infected with Lactobacillus sp.…”

Presence of Helicobacter pylori and H. suis DNA in Free-Range Wild Boars

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