Characterization of the native C-reactive protein (cCRP) and the corresponding liver mRNA in dogs

Jasensky, Anne-Katherine; Bondzio, Angelika; Murugaiyan, Jayaseelan; Siebert, U.; Roesler, Uwe; Kohn, Barbara; Einspanier, Ralf

doi:10.1016/j.bbrc.2014.08.090

Cited by 6 publications

(5 citation statements)

References 19 publications

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“…Dirofilariasis has been hypothesized to elicit the atypical benign clonal proliferation of plasma cells [ 18 ]. In dogs, γ globulin fractions are composed of various classes of Igs, and CRP also migrates in this fraction [ 19 , 20 ]. Moreover, the elevated γ globulin level in dogs infected with only D. immitis might be related to the high CRP concentration.…”

Section: Discussionmentioning

confidence: 99%

Serum protein profiles and C-reactive protein in natural canine filariasis

et al. 2021

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Background and Aim: Canine filariasis is caused by several species of filarial worms. The pathophysiological response to infection is mainly due to the filaria lifecycle. Laboratory detection methods to assess the pathological alterations characteristic of filariasis are needed urgently. Serum protein profiles and C-reactive protein (CRP) levels are used widely to diagnose several animal diseases. This study aimed to determine the serum protein profiles and CRP levels in dogs infected with Dirofilaria immitis or Brugia pahangi or both parasites. Materials and Methods: Blood samples were collected from 980 dogs presenting at animal hospitals and veterinary clinics in Bangkok and its vicinity. The presence of microfilaria in samples was determined using a buffy coat smear and staining with Wright–Giemsa. The sheathed and unsheathed microfilaria species were identified by acid phosphatase staining. Forty positive samples were tested. The serum protein profiles were identified by agarose gel electrophoresis. The CRP concentration was measured using a fluorescent immunoassay. Results: Albumin levels and albumin-to-globulin ratios were significantly lower, and total protein, β2 globulin, and γ globulin levels were significantly elevated in dogs infected with D. immitis and B. pahangi compared with reference values in normal dogs. The average CRP concentrations in dogs infected with D. immitis or B. pahangi were 69.9 and 12.9 mg/L, respectively. Conclusion: The total protein and γ globulin levels increased in canine filariasis compared with the normal reference range. The CRP concentration in dogs infected with D. immitis was extremely high, whereas that in dog infected with B. pahangi was normal.

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Section: Discussionmentioning

confidence: 99%

Serum protein profiles and C-reactive protein in natural canine filariasis

et al. 2021

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“…In dogs, C-reactive protein (CRP) migrates in this fraction as well. In horses it is found in the region between β-and γ-globulins (23,43,45,51).…”

Section: Analysis Of Electropherogram Patterns -Typical Electropherogrammentioning

confidence: 99%

Application of native agarose gel electrophoresis of serum proteins in veterinary diagnostics

Jania

Andraszek

2016

Journal of Veterinary Research

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Electrophoretic techniques, used to separate mixtures of electrically charged particles, are widely used in science. One of these techniques, native protein electrophoresis in an agarose gel, is applied in human and veterinary medicine. Changes in the proportions of individual protein fractions correspond to significant changes in the physiology of the body. Although the pattern obtained by electrophoretic separation rarely indicates a specific disease, it provides valuable information for the differential diagnosis. Decades of research on the types of patterns obtained in the case of particular diseases have led to the accumulation of substantial knowledge. The paper presents the available information on this topic. Serum protein electrophoresis is recommended in cases of increased levels of total protein in order to reveal the nature of the process. The basic information which can be obtained from electrophoretic separation includes the immune status of the organism. Both increased antigenic stimulation and immunodeficiency are clearly visible in electropherograms. Moreover, the level of heterogeneity of the corresponding protein fractions can help to distinguish between infectious diseases and cancer -multiple myeloma -the latter producing a homogeneous immunoglobulin fraction. Analysis of other protein fractions helps to detect or confirm an ongoing inflammatory process and provides information regarding liver function. Even when the concentration of total protein is within the reference range, this analysis can be recommended as a basic laboratory test.

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“…Esta proteína atua na proteção do organismo pela eliminação de agentes infecciosos por meio da ativação do sistema complemento com resultado de ativação da imunidade humoral e celular (CERÓN et al, 2005). Em eletroforese em gel de agarose SDS-page a proteína C-reativa é encontrada em dupla banda com 22 kDa e 24 kDa (JASENSKY et al, 2014).…”

Section: Proteína C Reativaunclassified

Proteínas De Fase Aguda (Pfa) Em Cães

Queiroz¹,

Castro²,

Ariza³

et al. 2016

Encicl. Biosf.

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RESUMOAs proteínas de fase aguda (PFAs) são biomarcadores que podem ser utilizados em diversos processos inflamatórios ou infecciosos como ferramentas de diagnóstico precoce ou indicadores de prognóstico e para acompanhamento da evolução da doença e sucesso do tratamento empregado. A resposta de fase aguda (RFA) é considerada uma resposta inespecífica do organismo diante de alguma injúria celular ou tecidual e, essa resposta se apresenta com aumento ou redução das PFAs. As PFAs podem ser classificadas como positivas (que aumentam a concentração durante a RFA), ou negativas (que reduzem sua concentração durante a RFA), e como maiores ou menores, de acordo com a amplitude de aumento ou redução diante de uma RFA. A técnica padrão para identificação das PFAs é a eletroforese, entretanto, algumas PFAs podem ser identificadas e quantificadas por métodos de ELISA, radioimunoensaio, nefelometria, imunoturbidimetria e análise de RNA. As principais PFAs estudadas em cães são: proteína C reativa, amilóide A sérica, haptoglobina, alfa-1 glicoproteína ácida, ceruloplasmina, fibrinogênio, albumina e transferrina. Os estudos sobre as PFAs em cães ainda são escassos, havendo uma grande área ainda a ser explorada. PALAVRAS-CHAVE: biomarcadores, caninos, resposta de fase aguda. ACUTE PHASE PROTEINS IN DOGSABSTRACT Acute phase proteins (AFP) are biomarkers that can be used in infectious processes as early diagnostic tools or prognostic indicators and for monitoring disease progression and success of the treatment. The acute phase response (APR) is considered a non-specific response of the body before any cell or tissue injury, and this response appears to increase or decrease the PFAs. The AFP can be classified as positive (rising concentration during APR) or negative (reducing their concentration during APR) and as larger or smaller, according to its amplitude increase or decrease before a FRG. The standard technique is to identify the AFP electrophoresis, however, some AFP can be identified and quantified by ELISA

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Characterization of the native C-reactive protein (cCRP) and the corresponding liver mRNA in dogs

Cited by 6 publications

References 19 publications

Serum protein profiles and C-reactive protein in natural canine filariasis

Serum protein profiles and C-reactive protein in natural canine filariasis

Application of native agarose gel electrophoresis of serum proteins in veterinary diagnostics

Proteínas De Fase Aguda (Pfa) Em Cães

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