Characterization of the mucocutaneous junction of the human eyelid margin and meibomian glands with different biomarkers

Tektas, Ozan Y.; Yadav, Ajay; Garreis, Fabian; Schlötzer‐Schrehardt, Ursula; Schicht, Martin; Hampel, Ulrike; Bräuer, Lars; Paulsen, Friedrich

doi:10.1016/j.aanat.2012.07.004

Cited by 24 publications

(33 citation statements)

References 55 publications

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“…To help characterize the anatomical location of cells in the human MG, we used antibodies cytokeratins 6 (K6) and 14 (K14) to identify ductal 12, 13, as well as all 14, 15, epithelial cells, respectively.…”

Section: Introductionmentioning

confidence: 99%

Biomarkers for Progenitor and Differentiated Epithelial Cells in the Human Meibomian Gland

Xie

Sullivan

Chen

et al. 2018

Stem Cells Translational Medicine

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The meibomian gland (MG) is a sebaceous gland that secretes through a holocrine process. Because such secretion requires the destruction of MG acinar epithelial cells, they need constant renewal and differentiation. The processes that promote these regenerative events in the human MG are unknown, nor is it known how to distinguish MG progenitor and differentiated cells. We discovered that Lrig1 and DNase2 serve as biomarkers for human MG progenitor and differentiated cells, respectively. Lrig1 is expressed in MG basal epithelial cells in the acinar periphery, a location where progenitor cells originate in sebaceous glands. DNase2 is expressed in the differentiated epithelial cells of the MG central acinus. Furthermore, proliferation stimulates, and differentiation suppresses, Lrig1 expression in human MG epithelial cells. The opposite is true for DNase2 expression. Our biomarker identification may have significant value in clinical efforts to restore MG function and to regenerate MGs after disease‐induced dropout. Stem Cells Translational Medicine 2018;7:887–892

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Section: Introductionmentioning

confidence: 99%

Biomarkers for Progenitor and Differentiated Epithelial Cells in the Human Meibomian Gland

Xie

Sullivan

Chen

et al. 2018

Stem Cells Translational Medicine

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“…29 However, it remained undetectable in Alvetex-cultured HMGECs, even after long-term air-lift culture. Since CK1 is one of the best-known markers of fully keratinized epithelium and detected in the orifices of meibomian duct, this suggests that air-liquid interface culture alone does not lead to keratinization in HMGECs.…”

Section: Discussionmentioning

confidence: 99%

“…Accordingly, CK10 was detected only in the ductal epithelium of human meibomian gland, but not in the meibocytes. 29 Hence, several bodies of evidence indicate that CK10 has the potential to serve as a biomarker to identify human ductal epithelial cells and to determine their differentiation status. In this study CK10 expression was mildly inducible on the apical surface in response to air exposure.…”

Section: Discussionmentioning

confidence: 99%

“…After counterstaining with hemalum, sections were coverslipped with Aquatex (Merck Millipore, Darmstadt, Germany). Sections of human eyelids taken from donor cadavers served as positive control 29 and were used in accordance with the Declaration of Helsinki. Two negative control sections were used: One was incubated with the secondary antibody only, and the other with the primary antibody only.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…29 In brief, after fixation, 3D-cultured HMGECs in Alvetex were washed with PBS three times. The membrane scaffolds were removed using a scalpel and subsequently embedded in optimal cutting temperature (OCT) compound (Tissue-Tek OCT Compound, Sakura Finetek USA, Inc., Torrance, CA, USA) and frozen at À208C.…”

Section: Immunohistochemistrymentioning

confidence: 99%

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Differentiation Patterns of Immortalized Human Meibomian Gland Epithelial Cells in Three-Dimensional Culture

Asano

Hampel

Garreis

et al. 2018

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. To establish a simplified three-dimensional (3D) meibomian gland culture model using a meibomian gland epithelial cell (HMGEC) line that might be a useful tool to gain deeper insights into meibomian gland dysfunction. For this purpose, 3D differentiation patterns and growth characteristics of HMGECs were studied on various membranes/scaffolds as well as in hanging drops.METHODS. Several types of inserts consisting of different materials (Millicell-HA, Millicell-PCF, ThinCert, and Alvetex) as well as hanging drop culture were analyzed. Culture conditions were optimized employing exposure to air (air-lift) and different cell culture media for a maximum of 28 days. To characterize cell differentiation in the developed 3D model, the expression pattern of cytokeratins was investigated by immunohistochemistry. Sudan III staining was performed for detection of lipid formation and transmission electron microscopy (TEM) was used for ultrastructural analysis. RESULTS.Only Alvetex scaffolds and the hanging drop method revealed satisfactory results with regard to 3D culture. Continuous use of proliferation medium (serum-free keratinocyte medium containing epidermal growth factor and bovine pituitary extract) and air-lift were important steps for HMGEC differentiation in 3D culture. However, HMGECs only reached a differentiating state and never became mature or hypermature. When cultured in hanging drops, HMGECs showed serum-induced keratinization processes. CONCLUSIONS.HMGECs have the capability to differentiate in a long-term 3D culture, especially when adapted to an air-rich environment. However, even in the 3D format, HMGECs only reach a state of differentiating meibocytes.

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Grundlagen

Dahlmann¹

2019

Sicca-Syndrom

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Characterization of the mucocutaneous junction of the human eyelid margin and meibomian glands with different biomarkers

Cited by 24 publications

References 55 publications

Biomarkers for Progenitor and Differentiated Epithelial Cells in the Human Meibomian Gland

Biomarkers for Progenitor and Differentiated Epithelial Cells in the Human Meibomian Gland

Differentiation Patterns of Immortalized Human Meibomian Gland Epithelial Cells in Three-Dimensional Culture

Grundlagen

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