Characterization of the morphological changes and fatty acid profile of developing Camelina sativa seeds

Rodríguez-Rodríguez, Manuel Fernando; Sánchez‐García, Alicia; Salas, Joaquı́n J.; Garcés, Rafael; Martı́nez-Force, Enrique

doi:10.1016/j.indcrop.2013.07.042

Cited by 80 publications

(80 citation statements)

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“…Accumulation of 20:1n-9, due to the activity of the enzyme responsible for the elongation, FAE1, has been suggested as a marker of oil accumulation (Girke et al, 2000), which our study supports. Also in accordance with previous studies on C. sativa (Rodriguez-Rodriguez et al, 2013;Pollard et al, 2015), 18:3n-3 accumulated at the highest rate and was the major fatty acid at the end of development. The general increase in 18:3n-3 and 20:1n-9 during development was seen in both total lipids and major TAG molecular species.…”

Section: Pdat Overexpression Increases Microsomal Tag Productionsupporting

confidence: 92%

“…1), which was expected as the C. sativa variety (CAM139) was chosen for, among other traits, its short life cycle and, thus, suitability for laboratory work. The lipid content of the mature seeds was still similar to what was shown by Rodriguez-Rodriguez et al (2013). Accumulation of 20:1n-9, due to the activity of the enzyme responsible for the elongation, FAE1, has been suggested as a marker of oil accumulation (Girke et al, 2000), which our study supports.…”

Section: Pdat Overexpression Increases Microsomal Tag Productionsupporting

confidence: 86%

“…sativa seeds are about 10 times the size of Arabidopsis seeds, enabling biochemical studies of the seed, which are not as easily performed on Arabidopsis. Wild-type C. sativa has a high content of a-linolenic acid (18:3n-3), 25% to 41% depending on the variety and growth conditions (Zubr, 1997;Rodriguez-Rodriguez et al, 2013), and also a high content of gondoic acid (20:1n-9) compared with other oilseed crops. The reasons for this high 18:3n-3 content is not clear.…”

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Two Acyltransferases Contribute Differently to Linolenic Acid Levels in Seed Oil

et al. 2017

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ORCID IDs: 0000-0001-6929-7859 (S.M.); 0000-0003-3152-0394 (D.S.); 0000-0001-8255-3255 (C.H.); 0000-0003-0489-3072 (K.C.); 0000-0002-9888-7003 (I.F.).Acyltransferases are key contributors to triacylglycerol (TAG) synthesis and, thus, are of great importance for seed oil quality. The effects of increased or decreased expression of ACYL-COENZYME A:DIACYLGLYCEROL ACYLTRANSFERASE1 (DGAT1) or PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASE (PDAT) on seed lipid composition were assessed in several Camelina sativa lines. Furthermore, in vitro assays of acyltransferases in microsomal fractions prepared from developing seeds of some of these lines were performed. Decreased expression of DGAT1 led to an increased percentage of 18:3n-3 without any change in total lipid content of the seed. The tri-18:3 TAG increase occurred predominantly in the cotyledon, as determined with matrix-assisted laser desorption/ionization-mass spectrometry, whereas species with two 18:3n-3 acyl groups were elevated in both cotyledon and embryonal axis. PDAT overexpression led to a relative increase of 18:2n-6 at the expense of 18:3n-3, also without affecting the total lipid content. Differential distributions of TAG species also were observed in different parts of the seed. The microsomal assays revealed that C. sativa seeds have very high activity of diacylglycerol-phosphatidylcholine interconversion. The combination of analytical and biochemical data suggests that the higher 18:2n-6 content in the seed oil of the PDAT overexpressors is due to the channeling of fatty acids from phosphatidylcholine into TAG before being desaturated to 18:3n-3, caused by the high activity of PDAT in general and by PDAT specificity for 18:2n-6. The higher levels of 18:3n-3 in DGAT1-silencing lines are likely due to the compensatory activity of a TAG-synthesizing enzyme with specificity for this acyl group and more desaturation of acyl groups occurring on phosphatidylcholine.

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Section: Pdat Overexpression Increases Microsomal Tag Productionsupporting

confidence: 92%

Section: Pdat Overexpression Increases Microsomal Tag Productionsupporting

confidence: 86%

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confidence: 99%

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Two Acyltransferases Contribute Differently to Linolenic Acid Levels in Seed Oil

et al. 2017

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“…Camelina accumulates high levels of oil in its seeds, representing between 15.5% and 41.7% of the seed weight, which are rich in ω-3 and ω-6 fatty acids. Typically, the major fatty acid species in Camelina oil are palmitic acid (16:0, 6.8% of the total fatty acids), stearic acid (18:0, 2.7%), oleic acid (18:1, 16.7%), linoleic acid (18:2, 21.9%), linolenic acid (18:3, 29.3%), gondoic acid (20:1, 13.9%) and erucic acid (22:1, 2.8%: Rodríguez-Rodríguez et al, 2013). The high percentage of polyunsaturated fatty acids in Camelina oil makes it unstable and inappropriate as a source of biodiesel or as a biolubricant stock (Fröhlich and Rice, 2005;Ciubota-Rosie et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Acyl-ACP thioesterases from Camelina sativa: Cloning, enzymatic characterization and implication in seed oil fatty acid composition

et al. 2014

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Acyl-acyl carrier protein (ACP) thioesterases are intraplastidial enzymes that terminate de novo fatty acid biosynthesis in the plastids of higher plants by hydrolyzing the thioester bond between ACP and the fatty acid synthesized. Free fatty acids are then esterified with coenzyme A prior to being incorporated into glycerolipids. Acyl-ACP thioesterases are classified into two families, FatA and FatB, which differ in their amino acid sequence and substrate specificity. Here, the FatA and FatB thioesterases from Camelina sativa seeds, a crop of interest in plant biotechnology, were cloned, sequenced and characterized. The mature proteins encoded by these genes were characterized biochemically after they were heterologously expressed in Escherichia coli and purified. Camelina sativa contained three different alleles of both the FatA and FatB genes. These genes were expressed most strongly in expanding tissues in which lipids are very actively synthesized, such as developing seed endosperm. The CsFatA enzyme displayed high catalytic efficiency on oleoyl-ACP and CsFatB acted efficiently on palmitoyl-ACP. The contribution of these two enzymes to the synthesis of Camelina sativa oil was discussed in the light of these results.

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“…O/L variances among sampling regions NJ (0.89), TA (0.88) and LF (0.87) were obviously higher than among other regions (Table 2). Meanwhile, thermo-oxidation resistance and low solidification point as good tribological properties of oils are characterized by low C20-24/C16-18 and low PUFA/MUFA ratios (Rodríguez-Rodríguez et al, 2013).…”

Section: Fatty Acid Compositionmentioning

confidence: 99%

Variability of seed oil content and fatty acid composition in Shantung maple (Acer truncatum Bunge) germplasm for optimal biodiesel production

Qiao¹,

Ye²,

Si³

et al. 2017

Afr. J. Biotechnol.

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Shantung maple seed oil methyl esters have emerged as the potential feedstock for producing biodiesel. The goal of this work was to assess variations in seed oil content and fatty acid compositions for optimal biodiesel production among 138 Shantung maple accessions native to 14 regions of China. /s) confirmed that Shantung maple methyl esters are suitable for biodiesel production, and correlation analysis showed that the accession with high monounsaturated fatty acid content was suitable as optimal germplasm resources for biodiesel production. DQTL, YS and Taian, Shandong (TA) regions was considered the best plantation, and DQTL-1, DQTL-6, DQTL-8, YS-6, and TA-10 germplasm accessions generated oil with optimal properties for biodiesel production. These results could guide future development of Shantung maple seed oil for improved biodiesel production.

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Characterization of the morphological changes and fatty acid profile of developing Camelina sativa seeds

Cited by 80 publications

References 20 publications

Two Acyltransferases Contribute Differently to Linolenic Acid Levels in Seed Oil

Two Acyltransferases Contribute Differently to Linolenic Acid Levels in Seed Oil

Acyl-ACP thioesterases from Camelina sativa: Cloning, enzymatic characterization and implication in seed oil fatty acid composition

Variability of seed oil content and fatty acid composition in Shantung maple (Acer truncatum Bunge) germplasm for optimal biodiesel production

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