Characterization of the modified Hodge test-positive isolates of Enterobacteriaceae in Taiwan

Abstract

Our data show that ESBLs, AmpC, and imipenemase-8 (IMP-8) carbapenemase coupled with decreased expression of outer membrane protein were prevalent in Enterobacteriaceae isolates testing positive for the modified Hodge test in Taiwan.

Colistin-sparing regimens against Klebsiella pneumoniae carbapenemase-producing K. pneumoniae isolates: Combination of tigecycline or doxycycline and gentamicin or amikacin

“…1,2 Additional carbapenemases, including Klebsiella pneumoniae carbapenemases (KPCs), emerged in Gram-negative bacteria due to extensive use of carbapenems. 3 A national surveillance of CRE in Taiwan in 2011 found a clonal dissemination of KPC-2-producing isolates during the time period, and the prevalence rate of KPCproducing K. pneumoniae was 22.3% in Taiwan in 2012. 4e6 The optimal treatment for infections due to carbapenemase-producing organisms is uncertain.…”

Comparison of Test Methods for Detecting Metallo-β-Lactamase-Producing Gram-Negative Bacteria

“…Both the Etest and Cica-Beta test are indeed simple and rapid screening methods, which are easy to implement into a clinical laboratory, although they are not specific for the detection of MBL producers, particularly strains coproducing plural classes of b-lactamases. The modified Hodge test is also a simple and inexpensive assay, but it may produce false-positive results in ESBL-producing clinical isolates (12,13) and negative or weakly positive results in NDM producers. Although MEPM showed the best performance in combination with SMA in detecting MBL-producing strains coproducing other classes of b-lactamases, the combination of SMA and CAZ among 56 strains provided the highest overall Se (87.5z) and Sp (100z) for the detection of MBL producers.…”

Identification of the first imported KPC-3 Klebsiella pneumoniae from the USA to Taiwan

“…Despite antibiotic therapy including linezolid, fluconazole, tigecycline, ciprofloxacin, fosfomycin and inhaled colistin, the patient died. The K. pneumoniae isolate was positive for carbapenemase production by the modified Hodge test [7,8].…”