Characterization of the mitochondrial genome ofArge bellaWei & Du sp. nov. (Hymenoptera: Argidae)

Abstract

We describe Arge bella Wei & Du sp. nov., a large and beautiful species of Argidae from south China, and report its mitochondrial genome based on high-throughput sequencing data. We present the gene order, nucleotide composition of protein-coding genes (PCGs), and the secondary structures of RNA genes. The nearly complete mitochondrial genome of A. bella has a length of 15,576 bp and a typical set of 37 genes (22 tRNAs, 13 PCGs, and 2 rRNAs). Three tRNAs are rearranged in the A. bella mitochondrial genome as compared to the ancestral type in insects: trnM and trnQ are shuffled, while trnW is translocated from the trnW-trnC-trnY cluster to a location downstream of trnI. All PCGs are initiated by ATN codons, and terminated with TAA, TA or T as stop codons. All tRNAs have a typical cloverleaf secondary structure, except for trnS1. H821 of rrnS and H976 of rrnL are redundant. A phylogenetic analysis based on mitochondrial genome sequences of A. bella, 21 other symphytan species, two apocritan representatives, and four outgroup taxa supports the placement of Argidae as sister to the Pergidae within the symphytan superfamily Tenthredinoidea.

Peer Review #2 of "Complete mitochondrial genome sequence of Labriocimbex sinicus, a new genus and new species of Cimbicidae (Hymenoptera) from China (v0.2)"

“…TTA-Leu showed the highest RSCU of 5.04 (Table 6). Comparisons of the RSCU with those of C. lateralis and T. anthracinum showed a similar pattern for codon usage bias and reflected a significant correlation between codon preference and nucleotide composition, that is similar to other symphytan species (Dowton et al, 2009;Wei et al, 2010;2015;Korkmaz et al, 2015Korkmaz et al, , 2016Korkmaz et al, , 2017Song et al, 2015Song et al, , 2016Du et al, 2018;Ma et al, 2019;Tang et al, 2019). Codons rich in C and G, CGC-Arg and CTC-Leu was absent, CGG-Arg, GGC-Gly, AGC-Ser, ACG-Thr, CTG-Leu, GTC-Val, GTG-Val and TGC-Cys, were used once, AGG-Ser, TCG-Ser, TCC-Ser, CCG-Pro and GCG-Ala were rarely used, which is similar to both cimbicid mitochondrial genomes ( Table 6).…”

Characterization of the mitochondrial genome ofAnalcellicampa xanthosomagen. et sp. nov. (Hymenoptera: Tenthredinidae)

“…Eight helices (H563, H1775, H1830, H1925, H2023, H2043, H2547 and H2588) were highly conserved. Furthermore, some helices (H837, H991, H1196 and H2347) were highly variable in terms of their sequence and secondary structure compared with other insects (Gillespie et al, 2006; Du et al, 2018; Niu et al, 2019; Castro & Dowton, 2005; Dowton et al, 2009). Compared with A. mellifera , which harbored an A at position 75 (H235), an A at position 238 (H671) and an A at position 733 in H1835, A. luctifer , A. rufocephalus and T. tienmushana harbored a U at each site.…”

“…Genomes from four species were also used as outgroups (Mecoptera, Diptera, Megaloptera, and Coleoptera) (Table 1). The 27 species of Symphyta represented eight families: Tenthredinidae (Wei, Niu & Du, 2014; Wei, Wu & Liu, 2015; Song et al, 2015; Song et al, 2016; GY Niu, 2017, unpublished data), Cimbicidae (Song et al, 2016; Doğan & Korkmaz, 2017; YC Yan, 2019, unpublished data), Pergidae (Castro & Dowton, 2005), Orussidae (Dowton et al, 2009), Cephidae (Dowton et al, 2009; Korkmaz et al, 2015; Korkmaz et al, 2016; Korkmaz et al, 2017; Korkmaz et al, 2018), Argidae (Du et al, 2018), Megalodontesidae, and Pamphiliidae (Niu et al, 2019).…”

Peer Review #2 of "Characterization of the mitochondrial genome of Analcellicampa xanthosoma gen. et sp. nov. (Hymenoptera: Tenthredinidae) (v0.1)"

“…Eight helices (H563, H1775, H1830, H1925, H2023, H2043, H2547 and H2588) were highly conserved. Furthermore, some helices (H837, H991, H1196 and H2347) were highly variable in terms of their sequence and secondary structure compared with other insects (Gillespie et al, 2006;Du et al, 2018;Niu et al. 2019;Castro and Dowton, 2005;Dowton et al, 2009).…”

“…Genomes from four species were also used as outgroups (Mecoptera, Diptera, Megaloptera, and Coleoptera) ( Table 1). The 27 species of Symphyta represented eight families: Tenthredinidae (Wei et al, 2014(Wei et al, , 2015Song et al, 2015Song et al, , 2016, Cimbicidae (Song et al, 2016;Doğan & Korkmaz, 2017;Yan et al, in press), Pergidae (Castro and Dowton, 2005), Orussidae (Dowton et al, 2009), Cephidae (Dowton et al, 2009;Korkmaz et al, 2015Korkmaz et al, , 2016Korkmaz et al, , 2017Korkmaz et al, , 2018, Argidae (Du et al, 2018), Megalodontesidae, and Pamphiliidae (Niu et al 2019). Phylogenetic relationships within the suborder Symphyta were reconstructed using both BI and ML analyses (Figure 9).…”