Characterization of the microstructure of hydrazone crosslinked polysaccharide-based hydrogels through rheological and diffusion studies

Karvinen, Jennika; Ihalainen, Teemu O.; Calejo, Maria Teresa; Jönkkäri, Ilari; Kellomäki, Minna

doi:10.1016/j.msec.2018.10.048

Cited by 69 publications

(65 citation statements)

References 24 publications

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“…The theory is based on the assumption that the hydrogel is purely elastic and neglects any physical interactions between the polymer chains. The crosslinking density (n e , mol m À3 ) of the hydrogels was calculated as follows: 27 n e ¼ G RT…”

Section: Rheologymentioning

confidence: 99%

Rapid and cytocompatible cell-laden silk hydrogel formation via riboflavin-mediated crosslinking

et al. 2020

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Section: Rheologymentioning

confidence: 99%

Rapid and cytocompatible cell-laden silk hydrogel formation via riboflavin-mediated crosslinking

et al. 2020

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“…The use of FRAP for measuring diffusion of particles in a solvent with various viscosities has been validated (Braeckmans et al 2003). Karvinen et al (Karvinen et al 2019) studied the FRAP kinetics of fluorescein-labeled dextrans (FITC-dextran) where the mobilities of 4 different hydrogels are distinguished. FRAP analysis is shown to be capable of accurately monitoring the diffusion in various media (Picart et al 2005;Sustr et al 2015) and is an practical method to study gel healing due to its versatility and accuracy.…”

Section: Introductionmentioning

confidence: 99%

Confocal Analysis of CNC based Hydrogels and Suspensions

Aa¹,

As²,

Sh³

2021

Preprint

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Cellulose nano crystal (CNC) hydrogels, while mechanically weak, have unique properties such as easy synthesis, high water content, and biocompatibility. Further improvement is needed to make CNC hydrogels mechanically stable and self-healable. Herein, using quantitative fluorescence recovery after photobleaching (FRAP) analysis, we assess stability, collapse, and level of self-healing of CNC hydrogels with different CNC and sodium chloride (NaCl) concentrations. We use the mean signal intensity obtained by confocal laser scanning microscopy (CLSM) to measure signal loss of the samples made of CNC hydrogels of different CNC concentrations and as a function of initial gel height and NaCl loading. The CNC dynamics inside the gels based on universality curves is unraveled which links the zeta potentials to the immobile particle percentages and the storage moduli as a function of NaCl/CNC concentration ratio. FRAP recovery analysis shows that for the ratio of NaCl/CNC beyond 0.1, the mobility of the ensemble of CNC particles becomes severely restricted. Hydrogel samples with low CNC concentrations (6 g/L and 10 g/L) experience a more substantial collapse rate under gravity than the rate observed for samples with a high CNC concentration (30 g/L). Increasing the CNC concentration hinders particle mobility and thus impedes the self-healing process. Quantification of the gel collapse behavior of CNC gel and its self-healing property is critical in many applications, including water and air filters, oil spill sponges, and tissue engineering.

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“…From the elastic modulus, we can also estimate the average mesh size of the hydrogel network as

ξ = {(RT / G^{'} N_{normalA})}^{1 / 3}

, where R is the gas constant (8.13 J mol −1 K −1 ), T is the temperature (respectively 298.15 K and 308.15 K), G ′ is the elastic modulus and N A is the Avogadro constant. [ 24 ] With that simple estimate, the average mesh size at 25 °C is found to be 16 nm, whereas the one at 35 °C is 13 nm. This mesh size is reasonable, as the average distance between pNIPAAm dangling chains calculated through its molar concentration is 8 nm.…”

Section: Resultsmentioning

confidence: 96%

Reversible Hydrogels with Switchable Diffusive Permeability

Nicolella

Lauxen

Ahmadi

et al. 2021

Macro Chemistry & Physics

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Hydrogels are polymer networks swollen in water that are characterized by soft mechanics and high permeability. This makes them good candidates for separation and membrane technologies. The diffusion is controlled by the mesh size of the network, and this can be made tunable through the introduction of thermoresponsive polymers. However, this is still a developing field. To contribute to this development, a dual dynamic network is formed composed of four‐arm polyethylene glycol precursors in which each arm is functionalized with both a terpyridine moiety capable of forming reversible metal–ligand complexes along with branches of poly(N‐isopropylacrylamide) (pNIPAAm), which can be switched between expanded and collapsed states and therewith offers a path for switching the microscopic gel‐network mesh size, along with the macroscopic gel elasticity. The dually sensitive hydrogel has the capability of doubling its elastic modulus when going above the lower critical solution temperature (LCST) of pNIPAAm. In addition, the diffusive permeability of the hydrogel is switched upon change of temperature, whereby diffusants are trapped above the LCST of pNIPAAm. Moreover, it is possible to disassemble and reform the gel upon change of pH. With that, the hydrogel has potential application as a switchable and reversible membrane.

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Characterization of the microstructure of hydrazone crosslinked polysaccharide-based hydrogels through rheological and diffusion studies

Cited by 69 publications

References 24 publications

Rapid and cytocompatible cell-laden silk hydrogel formation via riboflavin-mediated crosslinking

Rapid and cytocompatible cell-laden silk hydrogel formation via riboflavin-mediated crosslinking

Confocal Analysis of CNC based Hydrogels and Suspensions

Reversible Hydrogels with Switchable Diffusive Permeability

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