Characterization of the microbial community structure in intestinal segments of yak (Bos grunniens)

Zhang, Lihong; Jiang, Xiong; Li, Aoyun; Waqas, Muhammad; Gao, Xing; Li, Kun; Xie, Gary; Zhang, Jialü; Mehmood, Khalid; Zhao, Suonan; Basang, Wangdui; Li, Jiakui

doi:10.1016/j.anaerobe.2019.102115

Cited by 55 publications

(46 citation statements)

References 31 publications

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“…This study revealed that Proteobacteria, Firmicutes, and Bacteroidetes were the three most preponderant phyla in the gut microbial community of all samples. Consistent with previous studies on other mammals, those phyla were also observed to be abundantly presented in the intestines of goat, cattle, yak, and sheep, suggesting their importance in intestinal ecology and function (40)(41)(42). Interestingly, although diarrhea did not alter the diversity of dominant bacteria phyla in G. camelopardalis, the proportion of some intestinal bacteria changed significantly.…”

Section: Discussionsupporting

confidence: 88%

Microbiome Analysis Reveals the Dynamic Alternations in Gut Microbiota of Diarrheal Giraffa camelopardalis

Song

Qin

et al. 2021

Front. Vet. Sci.

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The ruminant gut microbial community's importance has been widely acknowledged due to its positive roles in physiology, metabolism, and health maintenance. Diarrhea has been demonstrated to cause adverse effects on gastrointestinal health and intestinal microecosystem, but studies regarding diarrheal influence on gut microbiota in Giraffa camelopardalis have been insufficient to date. Here, this study was performed to investigate and compare gut microbial composition and variability between healthy and diarrheic G. camelopardalis. The results showed that the gut microbial community of diarrheal G. camelopardalis displayed a significant decrease in alpha diversity, accompanied by distinct alterations in taxonomic compositions. Bacterial taxonomic analysis indicated that the dominant bacterial phyla (Proteobacteria, Bacteroidetes, and Firmicutes) and genera (Escherichia Shigella and Acinetobacter) of both groups were the same but different in relative abundance. Specifically, the proportion of Proteobacteria in the diarrheal G. camelopardalis was increased as compared with healthy populations, whereas Bacteroidetes, Firmicutes, Tenericutes, and Spirochaetes were significantly decreased. Moreover, the relative abundance of one bacterial genus (Comamonas) dramatically increased in diarrheic G. camelopardalis, whereas the relative richness of 18 bacterial genera decreased compared with healthy populations. Among them, two bacterial genera (Ruminiclostridium_5 and Blautia) cannot be detected in the gut bacterial community of diarrheal G. camelopardalis. In summary, this study demonstrated that diarrhea could significantly change the gut microbial composition and diversity in G. camelopardalis by increasing the proportion of pathogenic to beneficial bacteria. Moreover, this study first characterized the distribution of gut microbial communities in G. camelopardalis with different health states. It contributed to providing a theoretical basis for establishing a prevention and treatment system for G. camelopardalis diarrhea.

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Section: Discussionsupporting

confidence: 88%

Microbiome Analysis Reveals the Dynamic Alternations in Gut Microbiota of Diarrheal Giraffa camelopardalis

Song

Qin

et al. 2021

Front. Vet. Sci.

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“…Roseburia is a common butyrate-producing bacteria in the intestinal tract (Hatziioanou et al 2013 ; Sheridan et al 2019 ). Ruminococcaceae are common in both rumen and hindgut of ruminants, playing important parts in degrading starch and cellulose (Zhang et al 2020 ). In another study, Han et al ( 2018 ) found that Ruminococcaceae is related to the balance of Treg/Th17, suggesting that Ruminococcaceae may be related to the immune system.…”

Section: Discussionmentioning

confidence: 99%

Effect of Broussonetia papyrifera silage on the serum indicators, hindgut parameters and fecal bacterial community of Holstein heifers

et al. 2020

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This study investigated the effects of substitution of whole corn silage (WCS) with Broussonetia papyrifera silage (BPS) in different ratios on the serum indicators, hindgut fermentation parameters (pH, ammoniacal nitrogen, and volatile fatty acids), and fecal bacterial community of Holstein heifers. Sixteen heifers (8-month-old, 220 ± 30 kg) were randomly divided into four treatments according to different BPS substitution ratios of feed basis (0%, 25%, 50%, and 75%). The experiment consisted of a 7-day preliminary feeding period and a 30-day experimental period. On the last day of the trial, the blood samples were collected from caudal vein, and the feces samples were collected from rectum. With the increasing of BPS content, the concentration of malondialdehyde (MDA) and interleukin-1β (IL-1β) in serum decreased (P < 0.05), and the immunoglobulin A (IgA) and IL-4 content of serum increased (P < 0.05); and the hindgut pH value increased (P < 0.05). 16S rRNA sequencing found that the dominant phyla were Firmicutes, Bacteroidetes, and Verrucomicrobia; and the dominant genera were Ruminococcaceae_UCG-005, Ruminococcaceae_UCG-010, and Rikenellaceae_RC9_gut_group. Linear Discriminant Analysis Effect Size (LEfSe) analysis found 12 differential operational taxonomic units (OTUs) which have strong correlation with some serum and hindgut indicators, and have the potential to be used as biomarkers. Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) found that BPS have impacts on the pathways, such as carbohydrate transport and metabolism, and promotes amino acid transport and metabolism. To sum up, inclusion of BPS in heifer diets can affect serum anti-oxidant and immune indicators, fecal parameters, composition and function of fecal microorganisms in Holstein heifers.

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Section: Discussionmentioning

confidence: 99%

Effect of Broussonetia papyrifera Silage on the Serum Indicators, Hindgut Parameters and Fecal Bacterial Community of Holstein Heifers

Tian

Chen

Zhu

et al. 2020

Preprint

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This study investigated the effects of substitution of whole corn silage (CS) with Broussonetia papyrifera silage (BPS) in different ratios on the serum indicators, hindgut fermentation parameters (pH, ammoniacal nitrogen, and volatile fatty acids), and fecal bacterial community of Holstein heifers. Sixteen heifers (8-month-old, 220±30 kg) were randomly divided into four treatments according to different BPS substitution ratios of feed basis (0%, 25%, 50%, and 75%). The experiment consisted of a 7-day preliminary feeding period and a 30-day experimental period. On the last day of the trial, the blood samples were collected from caudal vein, and the feces samples were collected from rectum. With the increasing of BPS content, the concentration of malondialdehyde (MDA) and interleukin-1β (IL-1β) in serum decreased (P<0.05), and the immunoglobulin A (IgA) and IL-4 content of serum increased (P<0.05); and the hindgut pH value increased (P<0.05). 16S rDNA sequencing found that the dominant phyla were Firmicutes, Bacteroidetes, and Verrucomicrobia; and the dominant genera were Ruminococcaceae_UCG-005, Ruminococcaceae_UCG-010, and Rikenellaceae_RC9_gut_group. Linear Discriminant Analysis Effect Size (LEfSe) analysis found 12 differential operational taxonomic units (OTUs) which have strong correlation with some serum and hindgut indicators. Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) found that BPS have impacts on the pathways, such as carbohydrate transport and metabolism, and promotes amino acid transport and metabolism. To sum up, inclusion of BPS in heifer diets can affect the fecal bacterial community, and further improve serum anti-oxidant and immune indicators in Holstein heifers.

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Characterization of the microbial community structure in intestinal segments of yak (Bos grunniens)

Cited by 55 publications

References 31 publications

Microbiome Analysis Reveals the Dynamic Alternations in Gut Microbiota of Diarrheal Giraffa camelopardalis

Microbiome Analysis Reveals the Dynamic Alternations in Gut Microbiota of Diarrheal Giraffa camelopardalis

Effect of Broussonetia papyrifera silage on the serum indicators, hindgut parameters and fecal bacterial community of Holstein heifers

Effect of Broussonetia papyrifera Silage on the Serum Indicators, Hindgut Parameters and Fecal Bacterial Community of Holstein Heifers

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