Characterization of the mechanisms involved in the gastric antisecretory effect of TLQP-21, a vgf-derived peptide, in rats

Sibilia, V.; Pagani, F.; Bulgarelli, Ilaria; Tulipano, Giovanni; Possenti, Roberta; Guidobono, F.

doi:10.1007/s00726-010-0818-6

Cited by 10 publications

(7 citation statements)

References 44 publications

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“…Intracerebroventricular administration of VGF-derived peptide TLQP-21 in mice increased energy expenditure and body temperature without altering circulating thyroid hormones (free T3 and T4) and prevented high-fat diet-induced obesity [41, 42]. Intracerebroventricularly injected TLQP-21 inhibited gastric emptying, decreased gastric acid secretion and reduced ethanol-induced gastric lesion in rats [43-45]. These functional activities were inhibited by cyclooxygenase (COX) inhibitors, suggesting the involvement of prostaglandins.…”

Section: Diverse Functional Roles Of Granins and Their Derivative Pepmentioning

confidence: 99%

Secretogranin III: a diabetic retinopathy-selective angiogenic factor

Webster

LeBlanc

et al. 2017

Cell. Mol. Life Sci.

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Secretogranin III (Scg3) is a member of the granin protein family that regulates the biogenesis of secretory granules. Scg3 was recently discovered as an angiogenic factor, expanding its functional role to extrinsic regulation. Unlike many other known angiogenic factors, the pro-angiogenic actions of Scg3 are restricted to pathological conditions. Among thousands of quantified endothelial ligands, Scg3 has the highest binding activity ratio to diabetic vs. healthy mouse retinas and lowest background binding to normal vessels. In contrast, vascular endothelial growth factor binds to and stimulates angiogenesis of both diabetic and control vasculature. Consistent with its role in pathological angiogenesis, Scg3-neutralizing antibodies alleviate retinal vascular leakage in mouse models of diabetic retinopathy and retinal neovascularization in oxygen-induced retinopathy mice. This review summarizes our current knowledge of Scg3 as a regulatory protein of secretory granules, highlights its new role as a highly disease-selective angiogenic factor, and envisions Scg3 inhibitors as "selective angiogenesis blockers" for targeted therapy.

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Section: Diverse Functional Roles Of Granins and Their Derivative Pepmentioning

confidence: 99%

Secretogranin III: a diabetic retinopathy-selective angiogenic factor

Webster

LeBlanc

et al. 2017

Cell. Mol. Life Sci.

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“…TLQP-21 exerts a number of biological functions including: chronic i.c.v. infusion increases energy expenditure and prevents the early phase of diet-induced obesity [12], peripheral infusion induces prolipolytic effect [13], it regulates food intake and body weight in Syrian hamsters but not in mice [14,15], it regulates gastric functions in vitro and in vivo [16,17,18], and data report the modulation of this peptide by feeding in gastric endocrine cells [19]. TLQP-21 did not show any activity on GH expression and secretion in porcine pituitary slices in vitro [20] and in vivo after chronic TLQP-21 i.v.…”

Section: Introductionmentioning

confidence: 99%

Neuropeptide TLQP-21, a VGF Internal Fragment, Modulates Hormonal Gene Expression and Secretion in GH3 Cell Line

Passeri¹,

Biondini

Mongiardi

et al. 2012

Neuroendocrinology

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In the present study we demonstrated that TLQP-21, a biologically active peptide derived from the processing of the larger pro-VGF granin, plays a role in mammotrophic cell differentiation. We used an established in vitro model, the GH3 cell line, which upon treatment with epidermal growth factor develops a mammotrophic phenotype consisting of induction of prolactin expression and secretion, and inhibition of growth hormone. Here we determined for the first time that during mammotrophic differentiation, epidermal growth factor also induces Vgf gene expression and increases VGF protein precursor processing and peptide secretion. After this initial observation we set out to determine the specific role of the VGF encoded TLQP-21 peptide on this model. TLQP-21 induced a trophic effect on GH3 cells and increased prolactin expression and its own gene transcription without affecting growth hormone expression. TLQP-21 was also able to induce a significant rise of cytoplasmic calcium, as measured by Fura2AM, due to the release from a thapsigargin-sensitive store. TLQP-21-dependent rise in cytoplasmic calcium was, at least in part, dependent on the activation of phospholipase followed by phosphorylation of PKC and ERK. Taken together, the present results demonstrate that TLQP-21 contributes to differentiation of the GH3 cell line toward a mammotrophic phenotype and suggest that it may exert a neuroendocrine role in vivo on lactotroph cells in the pituitary gland.

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“…This peptide is known to play a role in centrally stimulating the autonomic nervous system by means of central prostaglandins (PGs), in peripherally stimulating adrenomedullary activity and in adipose tissue catabolism [2]. In addition, TLQP-21 has been shown to stimulate the in vitro rat gastric fundus contraction, and to exert an inhibitory in vivo role in the central regulation of gastric emptying and secretion through a PG-mediated mechanism [30,32]. TLQP-21 exerts a protective action on the gastric mucosa exposed to ethanol and this effect is mediated by constitutive-derived NO and PGE 2 [31].…”

Section: Introductionmentioning

confidence: 99%