Characterization of the mechanical properties of human parietal bones preserved in modified larssen solution, formalin and as fresh frozen

Pekedis, Mahmut; Yörük, Mustafa Deniz; Binboğa, Erdal; Yıldız, Hasan; Bilge, Okan; Çelik, Servet

doi:10.1007/s00276-021-02762-1

Cited by 4 publications

(5 citation statements)

References 38 publications

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“…The exception was the 96% glycerine solution at T1 because immediately after removal of the pieces from the solution, the piece was not dry, producing a more reflective piece with a wetter aspect, negatively interfering with image quality. Other studies using visual analysis have reported similar results for bone tissue, regardless of the solution used for fixation (Pekedis et al, 2021;Watanabe et al, 2019). Moreover, the high mineralization and hardness of bone tissue compared to other tissues, along with a lower blood content, minimize dehydration and oxidation caused by the addition of fixatives, resulting in less colour distortion (Hayashi et al, 2016).…”

Section: Discussionmentioning

confidence: 69%

Exploring alternatives for securing anatomical structures in capturing digital images: A comparative analysis

Turchenski,

Franco,

Turchenski

et al. 2023

Anat Histol Embryol

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Teaching veterinary anatomy using digital platforms requires improved image quality, which may influence the fixation process. This study aimed to compare four embalming solutions for high‐colour‐quality images of different tissues compared to the original image. Four equine left pelvic limbs were cut into metameres and divided equally for application of 10% formaldehyde, 96% glycerine, 33% hypersaturated NaCl solution and modified Larssen solution, respectively, which was maintained for 3 days. After drying for 3 days at room temperature, photographs were obtained at time 0 (T0), without any fixation process (original colour); time 1 (T1), immediately after removal from the solutions; and every 24 h for 3 days (T2–T4). The image colour quality was investigated by digitally evaluating the cortical bone, tendon and bone marrow using histograms and CIEDE2000 as well as by 10 specialists in an online survey. CIEDE2000 and histograms revealed that all fixation solutions changed the original tissue colour at all the time points (p < 0.0001). According to the specialists, the 33% saline solution produced the best results compared to the original one. The modified Larssen solution demonstrated better results for the tendon, marrow and cortical bone at T3 (p = 0.0015). Considering the colour of digital images, the modified Larssen solution provided the best results; however, the visual evaluation by the specialists revealed the 33% saline solution as the best.

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Section: Discussionmentioning

confidence: 69%

Exploring alternatives for securing anatomical structures in capturing digital images: A comparative analysis

Turchenski,

Franco,

Turchenski

et al. 2023

Anat Histol Embryol

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“…During full-text review, study citations were examined, and 20 articles were identified and included. A total of 186 papers 29–31,33–215 were included for data extraction.…”

Section: Resultsmentioning

confidence: 99%

“…This study 92 also found that the viscoelastic properties of embalmed and fresh-frozen tissues were different. It should be noted that all studies, 166,188,202,206 except for 1, 92 examined how preservation methods affected bone. There is a clear gap in the availability of studies that assess the biomechanical properties of different preservation methods.…”

Section: Discussionmentioning

confidence: 99%

“…It is also unknown whether different preparations of extracted tissue (eg, embalmed, fresh-frozen, fresh) affect biomechanical properties. Five studies compared different preservation methods of cadavers and assessed how the biomechanical properties changed 92,166,188,202,206 . The consensus was that cadaveric tissues preserved in formalin tend to have different properties to fresh-frozen tissues 92,166,206 .…”

Section: Discussionmentioning

confidence: 99%

“…Five studies compared different preservation methods of cadavers and assessed how the biomechanical properties changed 92,166,188,202,206 . The consensus was that cadaveric tissues preserved in formalin tend to have different properties to fresh-frozen tissues 92,166,206 . Additionally, biomechanical properties of extracted tissues do not seem to be affected by the length of freezing time 188,202 .…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Applied use of biomechanical measurements from human tissues for the development of medical skills trainers: a scoping review

Kriener,

Whiting,

Storr

et al. 2023

JBI Evidence Synthesis

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Objective: The objective of this review was to identify quantitative biomechanical measurements of human tissues, the methods for obtaining these measurements, and the primary motivations for conducting biomechanical research. Introduction: Medical skills trainers are a safe and useful tool for clinicians to use when learning or practicing medical procedures. The haptic fidelity of these devices is often poor, which may be because the synthetic materials chosen for these devices do not have the same mechanical properties as human tissues. This review investigates a heterogenous body of literature to identify which biomechanical properties are available for human tissues, the methods for obtaining these values, and the primary motivations behind conducting biomechanical tests. Inclusion criteria: Studies containing quantitative measurements of the biomechanical properties of human tissues were included. Studies that primarily focused on dynamic and fluid mechanical properties were excluded. Additionally, studies only containing animal, in silico, or synthetic materials were excluded from this review. Methods: This scoping review followed the JBI methodology for scoping reviews and the Preferred Reporting Items for Systematic Reviews and Meta-Analysis extension for Scoping Reviews (PRISMA-ScR). Sources of evidence were extracted from CINAHL (EBSCO), IEEE Xplore, MEDLINE (PubMed), Scopus, and engineering conference proceedings. The search was limited to the English language. Two independent reviewers screened titles and abstracts as well as full-text reviews. Any conflicts that arose during screening and full-text review were mediated by a third reviewer. Data extraction was conducted by 2 independent reviewers and discrepancies were mediated through discussion. The results are presented in tabular, figure, and narrative formats. Results: Data were extracted from a total of 186 full-text publications. All of the studies, except for 1, were experimental. Included studies came from 33 different countries, with the majority of the studies coming from the United States. Human tissues samples were ex vivo, and the most commonly studied tissue type was musculoskeletal. In this study, nearly 200 unique biomechanical values were reported, and the most commonly reported value was Young’s (elastic) modulus. The most common type of mechanical test performed was tensile testing, and the most common reason for testing human tissues was to characterize biomechanical properties. Although the number of published studies on biomechanical properties of human tissues has increased over the past 20 years, there are many gaps in the literature. Of the 186 included studies, only 7 used human tissues for the design or validation of medical skills training devices. Furthermore, in studies where biomechanical values for human tissues have been obtained, a lack of standardization in engineering assumptions, methodologies, and tissue preparation may implicate the usefulness of these values. Conclusions: This review is the first of its kind to give a broad overview of the biomechanics of human tissues in the published literature. With respect to high-fidelity haptics, there is a large gap in the published literature. Even in instances where biomechanical values are available, comparing or using these values is difficult. This is likely due to the lack of standardization in engineering assumptions, testing methodology, and reporting of the results. It is recommended that journals and/or experts in engineering fields conduct further research to investigate the feasibility of implementing reporting standards. Review Registration: Open Science Framework osf.io/fgb34

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Modified Larssen solution (MLS)-fixed cadaver model for transoral endoscopic thyroidectomy vestibular approach (TOETVA) education: a feasibility study

et al. 2022

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Characterization of the mechanical properties of human parietal bones preserved in modified larssen solution, formalin and as fresh frozen

Cited by 4 publications

References 38 publications

Exploring alternatives for securing anatomical structures in capturing digital images: A comparative analysis

Exploring alternatives for securing anatomical structures in capturing digital images: A comparative analysis

Applied use of biomechanical measurements from human tissues for the development of medical skills trainers: a scoping review

Modified Larssen solution (MLS)-fixed cadaver model for transoral endoscopic thyroidectomy vestibular approach (TOETVA) education: a feasibility study

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