Characterization of the major outer membrane antigens of Treponema hyodysenteriae

Wannemuehler, Michael J.; Hubbard, R D; Greer, J M

doi:10.1128/iai.56.12.3032-3039.1988

Cited by 48 publications

(33 citation statements)

References 31 publications

(25 reference statements)

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“…Brachyspira hyodysenteriae strain B204 was obtained from the culture collection at the Veterinary Medical Research Institute. 18 Three-month old, cross-bred pigs (n ¼ 3)…”

Section: Animals Infection and Necropsy Proceduresmentioning

confidence: 99%

CD4⁺ T‐cell responses and distribution at the colonic mucosa during Brachyspira hyodysenteriae‐induced colitis in pigs

Hontecillas¹,

Bassaganya‐Riera

Wilson

et al. 2005

Immunology

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Summary The spirochaete Brachyspira hyodysenteriae causes swine dysentery, a severe colitis characterized by mucosal enlargement as a result of crypt elongation and epithelial necrosis. Most efforts to understand the pathogenesis of this disease have focused on the aetiological agent and its virulence factors. However, the host immune response has been considered an important factor in disease development. Previous research has shown that B. hyodysenteriae induces systemic CD4+ and γδ T‐cell responses after intramuscular immunization. Here, we have evaluated changes in the CD4+ and γδ T‐cell composition and distribution the different compartments of the colonic mucosa of pigs challenged with B. hyodysenteriae. We report that, in infected pigs, γδ T cells were significantly depleted from the epithelial layer, although their numbers were maintained in the lamina propria. In addition, CD4+ T cells aggregated in clusters located in the lamina propria and submucosa. Ex vivo analyses of CD4+ T‐cell responses to B. hyodysenteriae antigens correlated with the changes in the mucosal CD4+ T‐cell distribution observed in infected pigs; CD4+ T cells recovered from peripheral blood and colonic lymph nodes of infected pigs proliferated to B. hyodysenteriae antigens, whereas no differences were found in the γδ T‐cell responses between challenged and control groups. In addition, colonic lymph node CD4+ T cells had a predominant memory/activated phenotype. These results indicate that infection with B. hyodysenteriae induces a mucosal CD4+ T‐cell response and points to CD4+ T cells being important contributors to the immunopathogenesis of swine dysentery.

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“…Brachyspira hyodysenteriae strain B204 was obtained from the culture collection at the Veterinary Medical Research Institute. 18 Three-month old, cross-bred pigs (n ¼ 3)…”

Section: Animals Infection and Necropsy Proceduresmentioning

confidence: 99%

CD4⁺ T‐cell responses and distribution at the colonic mucosa during Brachyspira hyodysenteriae‐induced colitis in pigs

Hontecillas¹,

Bassaganya‐Riera

Wilson

et al. 2005

Immunology

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“…Reports differ as to whether spirochetes contain lipopolysaccharide (LPS) in their outer membrane. Leptospira interrogans, Treponema phagedenis (biotype Reiter), and Serpula (Treponema) hyodysenteriae apparently do contain an LPSlike substance (3,21,23,28,35,36,41,63,66,76,77), while Treponema pallidum apparently does not (3,63). There is conflicting evidence regarding the presence of LPS in Borrelia spp.…”

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confidence: 99%

Purification and immunological characterization of a major low-molecular-weight lipoprotein from Borrelia burgdorferi

1992

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Borrelia burgdorferi resembles gram-negative bacteria in having both cellular and outer membranes. We previously showed that a lipopolysaccharide (LPS)-like material could be extracted from B. burgdorferi with phenol-chloroform-petroleum ether (PCP). The PCP extract of B. burgdorferi exhibited biological activity in several in vitro assays (e.g., mitogenicity, pyrogenicity, and cytokine release). These activities suggested the presence of endotoxin. The PCP extract of B. burgdorieri, however, also contained a small amount of protein.Preliminary studies showed that monoclonal antibody prepared against this protein inhibited the mitogenic activity of the PCP extract toward murine spleen cells. The current study was therefore undertaken to characterize this protein and to establish methods for its separation from the LPS. The PCP-extracted protein consisted of a single, low-molecular-weight lipoprotein (apparent M,, 10,000 by sodium dodecyl sulfatepolyacrylamide gel electrophoresis) (SDS-PAGE). By protein analysis, it accounted for 2% of the dry weight of defatted cells, thus making it a major constituent of the spirochete. It was purified from the LPS by initial extraction into 10%lY Triton X-100 followed by immunoaffinity chromatography in the presence of detergent. On removal of the LPS, the purified lipoprotein formed aggregates stable to SDS-PAGE which were detectable on Western blots (immunoblots) probed with either the monoclonal antibody or polyclonal antiserum. From a plot of the aggregate molecular weight versus aggregate size, a monomer molecular weight of 7,500 was obtained. Indirect immunofluorescence with the monoclonal antibody showed that the lipoprotein was exposed at the surface of the spirochete in only a small percentage of cells. The lipoprotein was present in several strains of B. burgdorferi but absent in other Borrelia spp., treponemes, and gram-negative human pathogens, indicating species specificity. 4995 on August 4, 2020 by guest http://iai.asm.org/ Downloaded from

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“…T. hyodysenteriae B204 (serotype 2) was grown anaerobically in Trypticase soy broth (BBL Microbiology Systems, Cockeysville, Md.) supplemented with 5% horse serum (HyClone labs, Logan, Utah) and 5% yeast extract (BBL) as previously described (33 Mice used in the challenge studies were 6 to 10 weeks of age.…”

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confidence: 99%

Effect of Treponema hyodysenteriae infection on mucosal mast cells and T cells in the murine cecum

Nibbelink

Wannemuehler

1990

Infect Immun

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The pathogenic mechanisms responsible for the development of lesions in swine and mice after infection with Treponema hyodysenteriae have not been fully characterized. The release of inflammatory mediators from mast cells has been postulated to play a role in lesion development during swine dysentery. Therefore, C3H/HeN mice were infected with T. hyodysenteriae, and mucosal mast cell (MMC) numbers were examined in cecal sections. An initial increase in MMC numbers from 13 to 22 per 50 crypt villus units was observed, but at 20 days postinfection the numbers significantly decreased (P < 0.05) to 5 MMC per 50 crypt villus units. Immunohistochemical analysis performed on cecal sections failed to show a significant change in lamina proprial T-lymphocyte subsets. Numbers of T. hyodysenteriae CFU recovered from the cecum were stable throughout the experimental time period. Mast cell-deficient W/WV mice and their mast cell-sufficient littermates were also infected to determine whether MMCs were necessary for the occurrence of T. hyodysenteriae-induced lesions. W/W' mice were as susceptible to infection as their normal littermates and developed similar macroscopic and microscopic lesions. These results indicate that changes in MMC numbers can be detected after an infection with T. hyodysenteriae; however, on the basis of observations of infected W/Wv mice, mast cells are not required for lesion development in the murine model.

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Characterization of the major outer membrane antigens of Treponema hyodysenteriae

Cited by 48 publications

References 31 publications

CD4⁺ T‐cell responses and distribution at the colonic mucosa during Brachyspira hyodysenteriae‐induced colitis in pigs

CD4⁺ T‐cell responses and distribution at the colonic mucosa during Brachyspira hyodysenteriae‐induced colitis in pigs

Purification and immunological characterization of a major low-molecular-weight lipoprotein from Borrelia burgdorferi

Effect of Treponema hyodysenteriae infection on mucosal mast cells and T cells in the murine cecum

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Characterization of the major outer membrane antigens of Treponema hyodysenteriae

Cited by 48 publications

References 31 publications

CD4+ T‐cell responses and distribution at the colonic mucosa during Brachyspira hyodysenteriae‐induced colitis in pigs

CD4+ T‐cell responses and distribution at the colonic mucosa during Brachyspira hyodysenteriae‐induced colitis in pigs

Purification and immunological characterization of a major low-molecular-weight lipoprotein from Borrelia burgdorferi

Effect of Treponema hyodysenteriae infection on mucosal mast cells and T cells in the murine cecum

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CD4⁺ T‐cell responses and distribution at the colonic mucosa during Brachyspira hyodysenteriae‐induced colitis in pigs

CD4⁺ T‐cell responses and distribution at the colonic mucosa during Brachyspira hyodysenteriae‐induced colitis in pigs