Characterization of the major allergens purified from the venom of the paper wasp Polistes gallicus

Pantera, Barbara; Hoffman, Donald R.; Carresi, Lara; Cappugi, Gianni; Turillazzi, Stefano; Manao, Giampaolo; Severino, Maurizio; Spadolini, I; Orsomando, Giuseppe; Moneti, Gloriano; Pazzagli, Luigia

doi:10.1016/j.bbagen.2003.07.001

Cited by 50 publications

(49 citation statements)

References 37 publications

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“…The similarity of the non-standardized venom reservoir areas in foundresses and workers could be explained by the need, independent of caste and role, to actively defend themselves and the colony, through attacking and stinging any intruders when other warning behaviours have not been a sufficient deterrent. The proteic component (Pantera et al, 2003) is probably the major part of the reservoir content and it is important in both foundresses and workers.…”

Section: Discussionmentioning

confidence: 99%

Caste differences in venom volatiles and their effect on alarm behaviour in the paper waspPolistes dominulus (Christ)

Bruschini

Cervo

Protti

et al. 2008

Journal of Experimental Biology

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SUMMARYFoundresses and workers of Polistes paper wasps show slight morphological and physiological differences. However, after the emergence of the workers, the castes can be readily discriminated by their behaviour: the dominant foundress is the principal egg-layer, whereas workers perform different tasks linked to colony development. Previous studies have demonstrated in this genus that defence of the colony by the workers is more effectively carried out by a collective response elicited by venom volatiles used as alarm pheromones. In the present study, gas chromatography-mass spectrometry analyses of the venom volatiles of foundresses and workers of Polistes dominulus (Christ) show predominantly quantitative differences. Spiroacetals, mainly (E,E)-2,8-dimethyl-1,7-dioxaspiro[5.5]undecane, are significantly higher in the venom volatiles fraction of workers, whereas the amount of N-(3-methylbutyl)acetamide is almost double in foundresses. On the basis of the chemical results, behavioural assays were performed on fifteen field colonies to test the alarm response of the resident wasps to venom extracts from foundresses and workers. Our behavioural results suggest that worker venom has a stronger alarm effect on the colonies than that of the foundresses, which seems unable to elicit the complete alarm response ending with a final attack and sting. The venom volatiles of P. dominulus workers serve mainly to alarm the colony whilst those of foundresses may also be linked to additional functions related to conspecific interactions.

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Section: Discussionmentioning

confidence: 99%

Caste differences in venom volatiles and their effect on alarm behaviour in the paper waspPolistes dominulus (Christ)

Bruschini

Cervo

Protti

et al. 2008

Journal of Experimental Biology

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“…However, in patient 12, who received double IT, the levels of sIgE to Polistes and to Vespula (Phadia) were higher after two years of IT, although the levels of sIgE to nVes v 1 and to nPol d 1 (ADVIA) had fallen. In patients vaccinated with P. dominulus but who had elevated sIgE to Vespula (patients 3,7,9,11), the levels of sIgE to Vespula also fell. In one patient (patient 13) who was vaccinated with Vespula, the levels of sIgE to Polistes also fell at two years.…”

Section: Discussionmentioning

confidence: 81%

“…6 Crossreactivity between wasps of the Vespinae family (Vespula, Dolichovespula and Vespa) and Polistes is generally lower than within the Vespinae family. 4,7 The phospholipases of Vespula and Polistes (Ves v 1 and Pol d 1) are very similar, but there is less similarity between antigen 5 of each venom 5 (Ves v 5 and Pol d 5) 3 ; for this reason, the Pol d 5 and Ves v 5 allergen components are considered to be markers of primary sensitisation to wasp venoms and, in particular, to Polistes and Vespula venoms.…”

Section: Introductionmentioning

confidence: 98%

Component-resolved diagnosis in vespid venom-allergic individuals

Galindo-Bonilla

Galan-Nieto

Alfaya

et al. 2015

Allergologia et Immunopathologia

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“…In wasp venoms the components are active amines (serotonin, histamine, tyramine, catecholamines); wasp kinins (similar in composition to bradykinin), which are mostly responsible for pain; and histamine-releasing peptides, which are responsible for the inflammatory response. The major allergens identified in wasp venoms are phospholipase A1, a hyaluronidase and a serine-protease (Pantera et al, 2003;Vetter et al, 1999).…”

Section: Bee and Wasp Venomsmentioning

confidence: 99%