Characterization of the Lipids of Six Strains of Bacteroides ruminicola

Abstract

The lipids of six strains of Bacteroides ruminicola were characterized. The nonpolar lipid accounted for 6 to 24% of the total lipid and was composed of diglycerides, triglycerides, and free fatty acids. The phospholipid fraction contained phosphatidylethanolamine, phosphosphingolipids, and trace quantities of phosphatidic acid. In three strains the phosphosphingolipid fraction made up more than half of the total lipid. The fatty acids in the nonpolar, acyland phosphosphingolipid consisted of a homologous series of branched and normal chains from 12 to 19 carbons. The long-chain base isolated from the phosphosphingolipids consisted of a homologous series of branched and normal chains from 14 to 24 carbons. Abraham (Ph.D. thesis, Univ. of Maryland, College Park, 1965) investigated the lipids isolated from a mixed population of rumen bacteria and found an unusual sphingolipid which contained ethanolamine. This ethanolamine-containing sphingolipid was later isolated from Bacteroides ruminicola strain 23 grown on a chemically defined lipid-free medium (J.

Long chain base composition of strains of three species of Sphingobacterium gen. nov.

“…Sphingolipids have been rare components in bacterial membrane systems, although ceramide phosphorylethanolamine and ceramide phosphorylglycerol were present in Bacteroides melaninogenicus, an absolutely anaerobic rumen bacterium [1][2][3]. They contain C16, C~7, C~8 and C~9 long-chain bases among which the branchedchain C19 and normal Cjs sphinganine predominate [14].…”

“…Sphingolipids are known to be among the important components of membrane lipids in higher animals and plants. In contrast, they are extremely rare in prokaryotic cells and reported to occur in only limited genera of bacteria [1][2][3][4][5][6]. Previously, we have found that the cellular lipids of Flavobacterium devorans ATCC10829, which belongs to the Pseudomonas-like group IIK-type 1, contained a unique sphingoglycolipid, ceramide glucuronic acid [7].…”

The Lipid Content of Mechanically Deboned Red Meats

“…Gas-liquid chromatography was performed with a Perkin Elmer model 881 gas chromatograph equipped with glass colums (1.8m) containing 10% SP-2340 or 10% SP-2330 on 100/120 Chromosorb W (Supelco Inc., Bellefonte, Pa.) as outlined by Kunsman (1973).…”

“…No further separation of the cerebrosides and sulfatides were attempted. The polar lipids were separated and quantified by two-dimentional thinlayer chromatography on RediCoats (Supelco, Inc., Bellefonte, Pa.) (Kunsman, 1973).…”

Separation and analysis of free ceramides containing 2-hydroxy fatty acids inSphingobacteriumspecies

“…Sphingolipids are already established to be important components in membrane lipids of higher organisms. However, they are extremely rare in bacterial cells and reported to occur only in limited genera [1][2][3][4][5][6]. Previously, we have reported that strains of Pseudomonas such as group II-K type 2, a Gram-negative, aerobic, opportunistic pathogen, contained sphingophospholipids [7,8] and there-fore, Yabuuchi et al [9] placed the organism in a new genus.…”