Characterization of the leafhopper mitogenome of <i>Mileewa alara</i> (Hemiptera: Cicadellidae: Mileewinae) and its phylogenetic analysis

He, Hailong; Yang, Maofa

doi:10.1080/23802359.2021.1906180

Cited by 4 publications

(8 citation statements)

References 14 publications

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“…Furthermore, the Old World taxa ( Atkinsoniella , Bothrogonia , Cicadella , Cofana , and Kolla ) consistently formed well-supported clades with the New World taxa (Cuerna, Draeculacephala , Homalodisca, and Paracrocampsa ) in all the phylogenetic trees. This was also observed in previous studies [ 51 , 52 , 54 , 55 , 77 , 78 ]. It is worth to notice that the genera included in the present study were all well-defined by previous studies.…”

Section: Resultssupporting

confidence: 91%

Comparative Analysis of Mitochondrial Genomes among Twelve Sibling Species of the Genus Atkinsoniella Distant, 1908 (Hemiptera: Cicadellidae: Cicadellinae) and Phylogenetic Analysis

Jiang

et al. 2022

Insects

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The herbivorous leafhopper genus Atkinsoniella Distant, 1908 (Hemiptera: Cicadellidae: Cicadellinae), a large genus of subfamily Cicadellinae, consists of 98 valid species worldwide and 88 species recorded in China. Some species of the genus are very similar in morphological characteristics, so they are difficult to identify accurately. In this study, 12 mitochondrial genomes of Atkinsoniella species with similar morphological characteristics were first obtained through high-throughput sequencing, which featured a typical circular molecule of 15,034–15,988 bp in length. The arrangement and orientation of 37 genes were identical to those of typical Cicadellidae mitogenomes. The phylogenetic relationship within the subfamily Cicadellinae was reconstructed using maximum-likelihood (ML) and Bayesian inference (BI) methods based on three concatenated datasets. The topological structures of the six obtained phylogenetic trees were highly consistent. The results suggested that Atkinsoniella was recovered as a monophyletic group and emerged as a sister group with the monophyletic clade of Bothrogonia, Paracrocampsa (part), and Draeculacephala (part). The branches of the 12 newly sequenced species were clearly separated, with most nodes receiving strong support in all analyses. In addition, the key to the 12 Atkinsoniella species was provided to identify species according to morphological characteristics. This study further promotes research on the classification, genetics, evolution, and phylogeny of the genus Atkinsoniella and subfamily Cicadellinae.

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Section: Resultssupporting

confidence: 91%

Comparative Analysis of Mitochondrial Genomes among Twelve Sibling Species of the Genus Atkinsoniella Distant, 1908 (Hemiptera: Cicadellidae: Cicadellinae) and Phylogenetic Analysis

Jiang

et al. 2022

Insects

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“…In the two newly sequenced mitogenomes, most of PCGs start with the typical codon ATN (ATA, ATT, ATG and ATC), whereas ND5 and ATP8 in M. rufivena began with a TTG codon. These abnormal initiation codons of ND5 and ATP8 are also reported in all other species of Mileewa, and this is a distinction between Mileewa and Ujna [14][15][16][17]. Three kinds of putative termination codon exist on two new mitogenomic sequences: TAG, TAA and a single T-.…”

Section: Protein-coding Genes and Codon Usagementioning

confidence: 51%

“…However, there are still many different opinions about the classification of Mileewinae, and the relationships among Cicadellinae, Typhlocybinae and Mileewinae have not yet been stated explicitly. In previous studies, three complete (M. albovittata, M. margheritae and M. ponta) mitogenomes and a partial mitogenome (M. alara) of Mileewa have been reported [14][15][16][17]. Analysis based on these mitochondria supported that Mileewinae was monophyletic, but it did not analyze the relationships between Mileewinae and other subfamilies.…”

Section: Introductionmentioning

confidence: 94%

Two Complete Mitochondrial Genomes of Mileewinae (Hemiptera: Cicadellidae) and a Phylogenetic Analysis

Zhang

2021

Insects

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More studies are using mitochondrial genomes of insects to explore the sequence variability, evolutionary traits, monophyly of groups and phylogenetic relationships. Controversies remain on the classification of the Mileewinae and the phylogenetic relationships between Mileewinae and other subfamilies remain ambiguous. In this study, we present two newly completed mitogenomes of Mileewinae (Mileewa rufivena Cai and Kuoh 1997 and Ujna puerana Yang and Meng 2010) and conduct comparative mitogenomic analyses based on several different factors. These species have quite similar features, including their nucleotide content, codon usage of protein genes and the secondary structure of tRNA. Gene arrangement is identical and conserved, the same as the putative ancestral pattern of insects. All protein-coding genes of U. puerana began with the start codon ATN, while 5 Mileewa species had the abnormal initiation codon TTG in ND5 and ATP8. Moreover, M. rufivena had an intergenic spacer of 17 bp that could not be found in other mileewine species. Phylogenetic analysis based on three datasets (PCG123, PCG12 and AA) with two methods (maximum likelihood and Bayesian inference) recovered the Mileewinae as a monophyletic group with strong support values. All results in our study indicate that Mileewinae has a closer phylogenetic relationship to Typhlocybinae compared to Cicadellinae. Additionally, six species within Mileewini revealed the relationship (U. puerana + (M. ponta + (M. rufivena + M. alara) + (M. albovittata + M. margheritae))) in most of our phylogenetic trees. These results contribute to the study of the taxonomic status and phylogenetic relationships of Mileewinae.

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“…1 and Table 1 . The species were medium length compared to other published Mileewinae species 18 – 22 . Each of the five newly sequenced mitogenomes contained 37 typical mitochondrial genes: 13 PCGs, two rRNA genes, 22 tRNA genes, and a CR (Fig.…”

Section: Resultsmentioning

confidence: 60%

“…The insect mitochondrial genomes are mostly 14.5–17 kb double-stranded circular molecules comprising 37 typical genes: 13 protein-coding genes (PCGs), two ribosomal RNA genes (rRNAs: s-rRNA; l-rRNA), 22 transfer RNA genes (tRNAs), and a control region (CR) 15 – 17 . To date, six Mileewinae species from China have been sequenced, and these sequences have been deposited in the NCBI database: Mileewa rufivena (MZ326689) 18 , Mileewa ponta (MT497465) 19 , Mileewa margheritae (MT483998) 20 , Mileewa albovittata (MK138358) 21 , Mileewa alara (MW533151) 22 and Ujna puerana (MZ326688) 18 . They are all from the genera Ujna and Mileewa , while sequences of Processina species have not been deposited in NCBI.…”

Section: Introductionmentioning

confidence: 99%

Characterization and phylogenetic implications of newly sequenced mitogenomes of Five Mileewa and Processina species from China (Hemiptera: Cicadellidae: Mileewinae)

Yan

et al. 2022

Sci Rep

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To accumulate evidence of the phylogeny of Mileewinae and the relationships among Mileewa, Processina, and Ujna genera, we sequenced the complete mitochondrial genomes of four Mileewa spp., namely, Mileewa mira, Mileewa lamellata, Mileewa sharpa, and Mileewa amplimacula. The first complete mitogenome of the genus Processina (P. sexmaculata), established by Yang, Deitz & Li from China and comprising five species, was also sequenced in this study. Annotation showed that the five mitogenomes were 14787 –15436 bp in length, and all harbored 37 typical genes. The AT content of the five mitogenomes ranged from 78.3% to 80.2%, which was similar to that of other sequenced Mileewinae species. For protein-coding genes (PCGs), ATN was the start codon, while atp8 and nad5 genes were initiated with TTG, and a great majority of them used TAA or TAG as stop codons, whereas cox2 and nad1 ended with an incomplete codon T–. All tRNAs had a typical cloverleaf secondary structure, except for trnS1, which had a reduced dihydrouridine arm. We further used 59 Membracoidea species and two outgroups to reconstruct phylogenetic trees based on 13 PCGs under an independent partition model with Bayesian inference and Maximum-likelihood methods. Among these two trees, each of the subfamilies Cicadellinae, Typhlocybinae, and Mileewinae were recovered as a monophyletic group with high support values, suggesting that Typhlocybinae was more ancient than Mileewinae and Cicadellinae. Within the Mileewinae subfamily, all species maintained the same relationships and topologies according to both the BI and ML analyses (PP > 0.8, BS > 83) as follows: (M. sharpa + (U. puerana + ((M. ponta + (M. mira + M. lamellata)) + ((M. albovittata + (M. margheritae + M. amplimacula)) + (M. rufivena + (P. sexmaculata + M. alara)))))), and the monophyly of the genera Processina, Mileewa and Ujna were not supported. This study further enriches the Mileewinae mitogenome database and will contribute to future research on the systematics, evolution, and classification of this group.

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Characterization of the leafhopper mitogenome of Mileewa alara (Hemiptera: Cicadellidae: Mileewinae) and its phylogenetic analysis

Cited by 4 publications

References 14 publications

Comparative Analysis of Mitochondrial Genomes among Twelve Sibling Species of the Genus Atkinsoniella Distant, 1908 (Hemiptera: Cicadellidae: Cicadellinae) and Phylogenetic Analysis

Comparative Analysis of Mitochondrial Genomes among Twelve Sibling Species of the Genus Atkinsoniella Distant, 1908 (Hemiptera: Cicadellidae: Cicadellinae) and Phylogenetic Analysis

Two Complete Mitochondrial Genomes of Mileewinae (Hemiptera: Cicadellidae) and a Phylogenetic Analysis

Characterization and phylogenetic implications of newly sequenced mitogenomes of Five Mileewa and Processina species from China (Hemiptera: Cicadellidae: Mileewinae)

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