Characterization of the Intestinal Absorption of Seven Flavonoids from the Flowers of Trollius chinensis Using the Caco-2 Cell Monolayer Model

Liu, Lijia; Guo, Li‐Na; Zhao, Can; Wu, Xiuwen; Wang, Rufeng; Liu, Chen

doi:10.1371/journal.pone.0119263

Cited by 21 publications

(14 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The apparent permeability coefficient ( P app ) values of the reference compounds, propranolol and atenolol, detected with the Caco-2 monolayers were (4.12 ± 0.17) × 10 −5 and (2.38 ± 0.24) × 10 −7 cm/s, respectively, which were basically consistent with the acceptable values reported in the literatures (Yee 1997 ; Liu et al. 2015 ). Therefore, this model was validated for the evaluation of the intestinal absorption potential of the compounds of interest.…”

Section: Resultssupporting

confidence: 89%

“…Our previous study has evidenced that the absorption of the flavonoids with 7-OH including 2″- O -β- l -galactopyranosylorientin, orientin, and vitexin involved transporter mediated efflux in addition to passive diffusion (Liu et al. 2015 ), the phenolic acids such as veratric acid and proglobeflowery acid were absorbed mainly through passive diffusion, and the alkaloid, trolline, was absorbed through an associative mechanism including both active and passive transports (Liu et al. 2014 ; Wu et al.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Investigation of the effective components of the flowers of Trollius chinensis from the perspectives of intestinal bacterial transformation and intestinal absorption

Guo

Qiao

et al. 2017

Pharmaceutical Biology

Self Cite

View full text Add to dashboard Cite

Context: The flowers of Trollius chinensis Bunge (Ranunculaceae), used for respiratory tract infections, mainly contain flavonoids, phenolic acids, and alkaloids; however, the effective components are debatable because of their unclear in vivo activities.Objective: This study investigates the effective components from the perspectives of biotransformation and absorption.Materials and methods: Both single person derived- and multiple people-derived intestinal florae were used to investigate the biotransformation of aqueous extract of the flowers of T. chinensis (AEOF) at the concentrations of 15.0, 30.0, and 60.0 mg/mL, respectively, for 72 h. Both human colon adenocarcinoma cell line (Caco-2) monolayers and everted gut sacs were employed to evaluate the intestinal absorption of the intestinal bacterial transformed AEOF at the concentrations of 10, 20, and 30 mg/mL, respectively, for 180 min.Results: 2″-O-β-l-Galactopyranosylorientin, orientin, vitexin, quercetin, veratric acid, proglobeflowery acid, and trolline in AEOF were not transformed by intestinal bacteria, while isoquercetin and trollioside were completely transformed. The Papp values of 2″-O-β-l-galactopyranosylorientin, orientin, and vitexin calculated based on the experimental data of intestinal absorption were at the levels of 10−5, whereas those of veratric acid, proglobeflowery acid, and trolline were at 10−4. The mass ratio of flavonoids to phenolic acids to alkaloids changed from 16:10:7 to 9:12:8 before and after absorption.Discussion and conclusion: The dominant position of flavonoids was replaced by phenolic acids after absorption. In addition to flavonoids which are usually considered as the dominant effective ones, phenolic acids and alkaloids should be also very important for the efficacy of these flowers.

show abstract

Section: Resultssupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Investigation of the effective components of the flowers of Trollius chinensis from the perspectives of intestinal bacterial transformation and intestinal absorption

Guo

Qiao

et al. 2017

Pharmaceutical Biology

Self Cite

View full text Add to dashboard Cite

show abstract

“…It is essential to find out why the low absorption of oral PTF occurred and to identify suitable pharmaceutical methods to improve the absorption of PTF and further enhance the anticancer activities. P-gp inhibitor (verapamil) [ 21 , 22 ] and MRPs inhibitor (indomethacin) [ 24 , 25 ] were employed to investigate whether the transport was affected by the efflux transporters, OATP inhibitor (indomethacin) [ 26 , 27 ] and SGLT1 inhibitor (phloridzin) [ 21 , 28 ] were chosen to study the effect of absorption transporters, and tight junction modulators (sodium dodecyl sulfate (SDS) and poly-l-lysine (PLL)) [ 29 , 30 ] were used to evaluate the role of paracellular permeability in the transport of these three compounds from PTF.…”

Section: Introductionmentioning

confidence: 99%

Transport of Corilagin, Gallic Acid, and Ellagic Acid from Fructus Phyllanthi Tannin Fraction in Caco‐2 Cell Monolayers

Mao

Zhao

et al. 2016

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Objective. To investigate the absorption property of the representative hydrolyzable tannin, namely corilagin, and its hydrolysates gallic acid (GA) and ellagic acid (EA) from the Fructus Phyllanthi tannin fraction (PTF) in vitro. Methods. Caco-2 cells monolayer model was established. Influences of PTF on Caco-2 cells viability were detected with MTT assay. The transport across monolayers was examined for different time points, concentrations, and secretory directions. The inhibitors of P-glycoprotein (P-gp), multidrug resistance proteins (MRPs), organic anion transporting polypeptide (OATP) and sodium/glucose cotransporter 1 (SGLT1), and tight junction modulators were used to study the transport mechanism. LC-MS method was employed to quantify the absorption concentration. Results. The apparent permeability coefficient (P app) values of the three compounds were below 1.0 × 10−6 cm/s. The absorption of corilagin and GA were much lower than their efflux, and the uptake of both compounds was increased in the presence of inhibitors of P-gp and MRPs. The absorption of EA was decreased in the company of OATP and SGLT1 inhibitors. Moreover, the transport of corilagin, GA, and EA was enhanced by tight junction modulators. Conclusion. These observations indicated that the three compounds in PTF were transported via passive diffusion combined with protein mediated transport. P-gp and MRPs might get involved in the transport of corilagin and GA. The absorption of EA could be attributed to OATP and SGLT1 protein.

show abstract

“…Sinapoyl glycosides of kaempferol and quercetina re less frequent in nature,a nd have been reported from Brassicaceaespecies such as Brassica napus [14], Lepidium sativum [15], and L. apetalum [16]. To the best of our knowledge,t his is the first report of flavonoid glycosidesa cylatedw ith 2-methylbutanoic or isobutyric acid moieties in aplant of the family of Brassicaceae.Such compounds have been previously reported from other botanical families such as Astereaceae [17], Ranunculaceae [18], and Convolvulaceae [19].…”

mentioning

confidence: 58%

New Acylated Flavonol Glycosides and a Phenolic Profile of Pritzelago alpina, a Forgotten Edible Alpine Plant

Corradi

Mieri

Cadisch

et al. 2016

Chemistry & Biodiversity

View full text Add to dashboard Cite

Thirteen acylated flavonoid glycosides, 1-13, including eleven new congeners, 3-13, were isolated from the aerial parts of Pritzelago alpina (Brassicaceae) by a combination of column chromatography on Sephadex LH-20, and preparative and semi-preparative HPLC. The structures were established by extensive NMR and MS experiments in combination with acid hydrolysis and sugar analysis by GC/MS. The new compounds were shown to be kaempferol and quercetin glycosides acylated for most of them by a branched short chain fatty acid or a hydroxycinnamic acid residue on the sugar portion. As shown by a HPLC-DAD analysis of a MeOH extract, these compounds are the main phenolic constituents in the aerial parts of the plant.

show abstract

Characterization of the Intestinal Absorption of Seven Flavonoids from the Flowers of Trollius chinensis Using the Caco-2 Cell Monolayer Model

Cited by 21 publications

References 14 publications

Investigation of the effective components of the flowers of Trollius chinensis from the perspectives of intestinal bacterial transformation and intestinal absorption

Investigation of the effective components of the flowers of Trollius chinensis from the perspectives of intestinal bacterial transformation and intestinal absorption

Transport of Corilagin, Gallic Acid, and Ellagic Acid from Fructus Phyllanthi Tannin Fraction in Caco‐2 Cell Monolayers

New Acylated Flavonol Glycosides and a Phenolic Profile of Pritzelago alpina, a Forgotten Edible Alpine Plant

Contact Info

Product

Resources

About