Characterization of the interface between hydroxyapatite coating film and bone: use of a new expression to correct matrix effects for PIXE measurements in thick samples embedded in resin

Abstract

Development of biomaterials for use as bone substitute requires better control of the process of materials degradation and bone substitution. At present, composite implants of alloy coated with bioceramic are being developed. In this paper, a cylinder made of TiAl 6 V 4 alloy coated with hydroxyapatite is used to simulate a hip prosthesis integration. Physico-chemical evaluations are needed to determine such an implant osseointegration process. Use of the particle induced x-ray emission (PIXE) method with histological sections results in quantitative cartography of elements at the bone-implant interface. The sensitivity of the PIXE method in measuring low concentrations of elements gives information to better understand bioresorption of calcium phosphate ceramics and events occurring at this dynamic interface. However, concentration evolution in different elements is still going on and reflects the ceramic resorption. In our case, the alpha parameter method based on experimental determination of the correction of matrix effects is useful as it allows data corrections to be calculated without hypotheses about matrix composition. In the present paper, we propose an expression to calculate directly the correction factor and an application to the characterization of the interface between hydroxyapatite coating film and bone.

An Original Method to Assess Short-Term Physicochemical Reactions at the Periphery of Bioactive Glass Particles in Biological Fluids

“…Bioceramics for hard tissue replacement can be divided into three categories: (a) bioinert ceramics, (b) surface-bioactive ceramics, and (c) resorbable bioactive ceramics. Bioinert ceramics are represented by alumina; surface-bioactive ceramics, by sintered hydroxyapatite, bioactive glasses, and glass ceramics; and resorbable bioactive ceramics, by low-crystalline hydroxyapatite, α or β-tricalcium phosphate, tetracalcium phosphate, and octocalcium phosphate. − …”

Evaluation of the Ca/P concentration ratio in hydroxyapatite by STEM-EDXS: influence of the electron irradiation dose and temperature processing

“…Due to its good biocompatibility and bioactivity, HA seems to have been the most investigated biomaterial in the last 12 years, mainly in powder form used for HA coating on a metallic substrate (in particular titanium) deposed by plasma spraying. The presence of this HA coating leads to two beneficial effects: the reduction of ionic release from the substrate, and a good biological binding if the interface HA substrate is stable [9]. To assess the bonding success, several methods are used to characterize the HA coating or HA particles.…”

PIXE characterization of by-products resulting from the zinc recycling of industrial cemented carbides

“…Proton induced X-ray emission (PIXE) techniques can detect trace elements lower than 500 lg g À1 [12] and can be used to characterize metallurgical samples in the form of accurate elemental mapping to significant depths of penetration, as has already been demonstrated for the WC system in previous work [10,13,14]. In this study, micro-PIXE techniques were used to determine elemental distribution (using two dimensional mapping) of dense and porous oversize materials, in order to understand the reasons behind their formation, the impact on the manufacturing process, as well as the potential implications for the powders that result from these materials.…”