Characterization of the Inflammatory Response Evoked by Bacterial Membrane Vesicles in Intestinal Cells Reveals an RIPK2-Dependent Activation by Enterotoxigenic Escherichia coli Vesicles

Thapa, Himadri B.; Kohl, Paul; Zingl, Franz G.; Fleischhacker, Dominik; Wolinski, Heimo; Kufer, Thomas A.; Schild, Stefan

doi:10.1128/spectrum.01115-23

Cited by 5 publications

(1 citation statement)

References 98 publications

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“… 104 , 141 The strong induction of the proinflammatory cytokine IL-8 by ETEC MVs was also reported to occur via the internalization of their MVs and subsequent delivery of their LPS (contained in the MVs) to intestinal epithelial cells, which is then recognized by novel caspase- and RIPK2-dependent pathways. 131 Underacylated LPS-derived ETEC OMVs showed similar uptake dynamics but less proinflammatory potency than MVs derived from WT ETEC, suggesting that this identification is likely due to the detection of the lipid A moiety.…”

Section: Mvs and Their Potential Role In Ibdmentioning

confidence: 93%

Bacterial membrane vesicles in the pathogenesis and treatment of inflammatory bowel disease

Olovo,

Wiredu Ocansey,

et al. 2024

Gut Microbes

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Inflammatory bowel disease (IBD) is a chronic and debilitating condition of relapsing and remitting inflammation in the gastrointestinal tract. Conventional therapeutic approaches for IBD have shown limited efficacy and detrimental side effects, leading to the quest for novel and effective treatment options for the disease. Bacterial membrane vesicles (MVs) are nanosized lipid particles secreted by lysis or blebbing processes from both Gram-negative and Gram-positive bacteria. These vesicles, known to carry bioactive components, are facsimiles of the parent bacterium and have been implicated in the onset and progression, as well as in the amelioration of IBD. This review discusses the overview of MVs and their impact in the pathogenesis, diagnosis, and treatment of IBD. We further discuss the technical challenges facing this research area and possible research questions addressing these challenges. We summarize recent advances in the diverse relationship between IBD and MVs, and the application of this knowledge as a viable and potent therapeutic strategy for IBD.

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Section: Mvs and Their Potential Role In Ibdmentioning

confidence: 93%

Bacterial membrane vesicles in the pathogenesis and treatment of inflammatory bowel disease

Olovo,

Wiredu Ocansey,

et al. 2024

Gut Microbes

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TrxR1 is involved in the activation of Caspase-11 by regulating the oxidative-reductive status of Trx-1

Bai,

Zhou,

et al. 2024

Redox Biology

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Proteomic and Metabolomic Profiling of Archaeal Extracellular Vesicles from the Human Gut

Weinberger,

Darnhofer,

Mertelj

et al. 2024

Preprint

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One potential mechanism for microbiome-host, and microbiome constituents’ interaction and communication involves extracellular vesicles (EVs). Here, for the first time, we report the capability of two M. smithii strains (ALI and GRAZ-2), Candidatus M. intestini, and Methanosphaera stadtmanae, as underrepresented components of the gut microbiome, to produce EVs. Interesting, size, morphology, and composition of AEVs were comparable to bacterial EVs, as indicated by ultrastructure, composition, proteomic and metabolomic analyses; however, EVs were substantially less prevalent in the studied Archaea. When looking at the proteomics more precisely, although AEVs from M. smithii ALI and M. intestini were found to be carrying unique proteins (n=135 and n=30, respectively), the shared proteins in AEVs within this genus (n=229), were mostly adhesins(/like) proteins, or proteins with IG-like domains. One remarkable observation was the uptake of AEVs obtained from Methanosphaera stadtmanae and the studied Methanobrevibacter species by human monocytes and the subsequent IL-8 secretion.

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Characterization of the Inflammatory Response Evoked by Bacterial Membrane Vesicles in Intestinal Cells Reveals an RIPK2-Dependent Activation by Enterotoxigenic Escherichia coli Vesicles

Cited by 5 publications

References 98 publications

Bacterial membrane vesicles in the pathogenesis and treatment of inflammatory bowel disease

Bacterial membrane vesicles in the pathogenesis and treatment of inflammatory bowel disease

TrxR1 is involved in the activation of Caspase-11 by regulating the oxidative-reductive status of Trx-1

Proteomic and Metabolomic Profiling of Archaeal Extracellular Vesicles from the Human Gut

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