Characterization of the Impact of Classical Cell‐culture Media on the Response of Electrochemical Sensors

Chmayssem, Ayman; Petit, Lauriane; Verplanck, Nicolas; Mourier, Véronique; Vignoud, Séverine; Vrana, Nihal Engin; Mailley, Pascal

doi:10.1002/elan.202100534

Cited by 10 publications

(11 citation statements)

References 40 publications

(49 reference statements)

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“…This device is a powerful toolkit for monitoring cell-culture growth and barrier tissues integrity. Additionally, these devices can be used to assess cell stress in a biomaterial risk assessment to complement the detection of inflammatory metabolites using multiparametric electrochemical sensing platforms [ 4 , 26 ]. Our strategy deals with the selection of highly processable carbon inks (which are blended with IrOx particles to decreases their interfacial impedance) with potentially low fabrication cost (involving printing technologies).…”

Section: Discussionmentioning

confidence: 99%

“…However, these tests offer limited insight into the functional properties of cells. This led to the development of complementary techniques for the biocompatibility testing of biomaterials that could provide more information on the overall health of cells and tissue [ 3 , 4 , 5 ]. However, these tests could be prohibitively complicated and expensive for screening a large number of conditions.…”

Section: Introductionmentioning

confidence: 99%

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New Microfluidic System for Electrochemical Impedance Spectroscopy Assessment of Cell Culture Performance: Design and Development of New Electrode Material

et al. 2022

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Electrochemical impedance spectroscopy (EIS) is widely accepted as an effective and non-destructive method to assess cell health during cell-culture. However, there is a lack of compact devices compatible with microfluidic integration and microscopy that could provide the real-time and non-invasive monitoring of cell-cultures using EIS. In this paper, we reported the design and characterization of a modular EIS testing system based on a patented technology. This device was fabricated using easily processable methodologies including screen-printing of the impedance electrodes and molding or micromachining of the cell culture chamber with an easy assembly procedure. Accordingly, to obtain processable, biocompatible and sterilizable electrode materials that lower the impact of interfacial impedance on TEER (Transepithelial electrical resistance) measurements, and to enable concomitant microscopy observations, we optimized the formulation of the electrode inks and the design of the EIS electrodes, respectively. First, electrode materials were based on carbon biocompatible inks enriched with IrOx particles to obtain low interfacial impedance electrodes approaching the performances of classical non-biocompatible Ag/AgCl second-species electrodes. Secondly, we proposed three original electrode designs, which were compared to classical disk electrodes that were optically compatible with microscopy. We assessed the impact of the electrode design on the response of the impedance sensor using COMSOL Multiphysics. Finally, the performance of the impedance spectroscopy devices was assessed in vitro using human airway epithelial cell cultures.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

New Microfluidic System for Electrochemical Impedance Spectroscopy Assessment of Cell Culture Performance: Design and Development of New Electrode Material

et al. 2022

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“…The contributions to that current from the oxidation of some endogenous reducing compound species, such as ascorbate, dopamine and some drugs, can furthermore compromise the selectivity of the glucose biosensors [ 17 , 18 ]. For this reason, different strategies have been developed to minimize this problem, including the use of low reduction potential mediators or by measuring the direct oxidation of the enzyme on the surface of the electrode, also achieved at a low potential [ 19 , 20 ]. These two strategies, respectively, constitute the principles of second and third generation electrochemical glucose biosensors [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Biosensing of Glucose Based on the Enzymatic Reduction of Glucose

Soranzo

Tahar

Chmayssem

et al. 2022

Sensors

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In this work, the enzyme aldehyde reductase, also known as aldose reductase, was synthesized and cloned from a human gene. Spectrophotometric measurements show that in presence of the nicotinamide adenine dinucleotide phosphate cofactor (NADPH), the aldehyde reductase catalyzed the reduction of glucose to sorbitol. Electrochemical measurements performed on an electrodeposited poly(methylene green)-modified gold electrode showed that in the presence of the enzyme aldehyde reductase, the electrocatalytic oxidation current of NADPH decreased drastically after the addition of glucose. These results demonstrate that aldehyde reductase is an enzyme that allows the construction of an efficient electrochemical glucose biosensor based on glucose reduction.

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“…To date, very few papers have reported on the influence of cell culture media on the performance of electrochemical sensors [ 68 , 69 ]. In this work, the effect of different culture media was studied in detail.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Quantification of H2O2 Released by Airway Cells Growing in Different Culture Media

et al. 2022

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Quantification of oxidative stress is a challenging task that can help in monitoring chronic inflammatory respiratory airway diseases. Different studies can be found in the literature regarding the development of electrochemical sensors for H2O2 in cell culture medium to quantify oxidative stress. However, there are very limited data regarding the impact of the cell culture medium on the electrochemical quantification of H2O2. In this work, we studied the effect of different media (RPMI, MEM, DMEM, Ham’s F12 and BEGM/DMEM) on the electrochemical quantification of H2O2. The used electrode is based on reduced graphene oxide (rGO) and gold nanoparticles (AuNPs) and was obtained by co-electrodeposition. To reduce the electrode fouling by the medium, the effect of dilution was investigated using diluted (50% v/v in PBS) and undiluted media. With the same aim, two electrochemical techniques were employed, chronoamperometry (CH) and linear scan voltammetry (LSV). The influence of different interfering species and the effect of the operating temperature of 37 °C were also studied in order to simulate the operation of the sensor in the culture plate. The LSV technique made the sensor adaptable to undiluted media because the test time is short, compared with the CH technique, reducing the electrode fouling. The long-term stability of the sensors was also evaluated by testing different storage conditions. By storing the electrode at 4 °C, the sensor performance was not reduced for up to 21 days. The sensors were validated measuring H2O2 released by two different human bronchial epithelial cell lines (A549, 16HBE) and human primary bronchial epithelial cells (PBEC) grown in RPMI, MEM and BEGM/DMEM media. To confirm the results obtained with the sensor, the release of reactive oxygen species was also evaluated with a standard flow cytometry technique. The results obtained with the two techniques were very similar. Thus, the LSV technique permits using the proposed sensor for an effective oxidative stress quantification in different culture media and without dilution.

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Characterization of the Impact of Classical Cell‐culture Media on the Response of Electrochemical Sensors

Cited by 10 publications

References 40 publications

New Microfluidic System for Electrochemical Impedance Spectroscopy Assessment of Cell Culture Performance: Design and Development of New Electrode Material

New Microfluidic System for Electrochemical Impedance Spectroscopy Assessment of Cell Culture Performance: Design and Development of New Electrode Material

Electrochemical Biosensing of Glucose Based on the Enzymatic Reduction of Glucose

Electrochemical Quantification of H2O2 Released by Airway Cells Growing in Different Culture Media

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