Characterization of the immunomodulatory and anti-<i>Helicobacter pylori</i> properties of the human gastric isolate <i>Lactobacillus rhamnosus</i> UCO-25A

García-Castillo, Valeria; Marín-Vega, Ana María; Ilabaca, Alejandra; Albarracín, Leonardo; Marcial, Guillermo; Kitazawa, Haruki; García-Cancino, Apolinaria; Villena, Julio

doi:10.1080/08927014.2019.1675153

Cited by 8 publications

(11 citation statements)

References 58 publications

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“…Earlier studies reported that L. salivarius WB 1004 [35], L. rhamnosus R0011 and L. acidophilus R0052 [36] were able to differentially modulate immune responses and diminish H. pylori colonization in mice. The beneficial modulation of the inflammatory immune response and the reduction of H. pylori adhesion has been also reported in in vitro experiments for probiotic strains such as L. bulgaricus [37] and L. rhamnosus UCO-25A [38]. Moreover, studies have proposed the inactivation of Smad7 and NF-κB signaling pathways [39] and the activation of SOCS-2/SOCS-3 signaling through STAT1/STAT3 activation and JAK2 inactivation [40] as the molecular mechanisms of action for the beneficial effects of probiotics.…”

Section: Discussionmentioning

confidence: 64%

The Exopolysaccharide of Lactobacillus fermentum UCO-979C Is Partially Involved in Its Immunomodulatory Effect and Its Ability to Improve the Resistance against Helicobacter pylori Infection

et al. 2020

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Lactobacillus fermentum UCO-979C (Lf979C) beneficially modulates the cytokine response of gastric epithelial cells and macrophages after Helicobacter pylori infection in vitro. Nevertheless, no in vivo studies were performed with this strain to confirm its beneficial immunomodulatory effects. This work evaluated whether Lf979C improves protection against H. pylori infection in mice by modulating the innate immune response. In addition, we evaluated whether its exopolysaccharide (EPS) was involved in its beneficial effects. Lf979C significantly reduced TNF-α, IL-8, and MCP-1 and augmented IFN-γ and IL-10 in the gastric mucosa of H. pylori-infected mice. The differential cytokine profile induced by Lf979C in H. pylori-infected mice correlated with an improved reduction in the pathogen gastric colonization and protection against inflammatory damage. The purified EPS of Lf979C reduced IL-8 and enhanced IL-10 levels in the gastric mucosa of infected mice, while no effect was observed for IFN-γ. This work demonstrates for the first time the in vivo ability of Lf979C to increase resistance against H. pylori infection by modulating the gastric innate immune response. In addition, we advanced knowledge of the mechanisms involved in the beneficial effects of Lf979C by demonstrating that its EPS is partially responsible for its immunomodulatory effect.

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Section: Discussionmentioning

confidence: 64%

The Exopolysaccharide of Lactobacillus fermentum UCO-979C Is Partially Involved in Its Immunomodulatory Effect and Its Ability to Improve the Resistance against Helicobacter pylori Infection

et al. 2020

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“…The capacity of both strains to modulate the immune response as the result of an infection by Helicobacter pylori has been evaluated in AGS cells (human gastric adenocarcinoma cell line) and THP-1 cells (human monocytic cell line). Both cell lines were demonstrated to increase the IL-10 and IFN-γ anti-inflammatory response as well as reduce the TNF-α IL-8 and IL-1b proinflammatory response, being the preventive model that provided the best results [ 115 , 116 , 117 ]. This is an important issue because the reduced production of inflammatory factors, particularly IL-8 and TNF-α, is associated with a less serious infection and ensuing sequels [ 115 , 117 ].…”

Section: Resultsmentioning

confidence: 99%

Can Probiotics, Particularly Limosilactobacillus fermentum UCO-979C and Lacticaseibacillus rhamnosus UCO-25A, Be Preventive Alternatives against SARS-CoV-2?

Valdebenito-Navarrete

Fuentes-Barrera

Smith

et al. 2023

Biology

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COVID-19, an infection produced by the SARS-CoV-2 virus in humans, has rapidly spread to become a high-mortality pandemic. SARS-CoV-2 is a single-stranded RNA virus characterized by infecting epithelial cells of the intestine and lungs, binding to the ACE2 receptor present on epithelial cells. COVID-19 treatment is based on antivirals and antibiotics against symptomatology in addition to a successful preventive strategy based on vaccination. At this point, several variants of the virus have emerged, altering the effectiveness of treatments and thereby attracting attention to several alternative therapies, including immunobiotics, to cope with the problem. This review, based on articles, patents, and an in silico analysis, aims to address our present knowledge of the COVID-19 disease, its symptomatology, and the possible beneficial effects for patients if probiotics with the characteristics of immunobiotics are used to confront this disease. Moreover, two probiotic strains, L. fermentum UCO-979C and L. rhamnosus UCO-25A, with different effects demonstrated at our laboratory, are emphasized. The point of view of this review highlights the possible benefits of probiotics, particularly those associated with immunomodulation as well as the production of secondary metabolites, and their potential targets during SARS-CoV-2 infection.

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“…Production of IL-10 is a marker of an anti-inflammatory response to Lactobacillus . This interleukin has previously been shown to be induced by Lactobacillus in other cell types including macrophage cell lines (RAW264.7 (mouse) and THP-1(human)), cervical tumor cells (HeLa), and colonic epithelial cells (Caco2) [ 36 , 44 , 45 ]. However, we observed little production of IL-10 in mock or following infection with all four strains of Lactobacillus in our study.…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus strains vary in their ability to interact with human endometrial stromal cells

Shiroda

Manning

2020

PLoS ONE

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The placental membranes that surround the fetus during pregnancy were suggested to contain a low abundance microbiota. Specifically, abundance of Lactobacillus , a probiotic and dominant member of the microbiome of the lower reproductive tract, has been shown to correlate with healthy, term pregnancies. We therefore sought to assess the interactions between four different Lactobacillus strains with immortalized decidualized endometrial cells (dT-HESCs), which were used as a model to represent the outermost layer of the placental membranes. Notably, we demonstrated that all four Lactobacillus strains could associate with dT-HESCs in vitro . L . crispatus was significantly more successful (p < 0.00005), with 10.6% of bacteria attaching to the host cells compared to an average of 0.8% for the remaining three strains. The four strains also varied in their ability to form biofilms. Dependent on media type, L . reuteri 6475 formed the strongest biofilms in vitro . To examine the impact on immune responses, levels of total and phosphorylated protein p38, a member of the Mitogen Activated Protein Kinase (MAPK) pathway, were examined following Lactobacillus association with dT-HESCs. Total levels of p38 were reduced to an average of 44% that of the cells without Lactobacillus (p < 0.05). While a trend towards a reduction in phosphorylated p38 was observed, this difference was not significant (p > 0.05). In addition, association with Lactobacillus did not result in increased host cell death. Collectively, these data suggest that varying types of Lactobacillus can attach to the outermost cells of the placental membranes and that these interactions do not contribute to inflammatory responses or host cell death. To our knowledge this is the first in vitro study to support the ability of Lactobacillus to interact with placental cells, which is important when considering its use as a potential probiotic within the reproductive tract.

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Characterization of the immunomodulatory and anti-Helicobacter pylori properties of the human gastric isolate Lactobacillus rhamnosus UCO-25A

Cited by 8 publications

References 58 publications

The Exopolysaccharide of Lactobacillus fermentum UCO-979C Is Partially Involved in Its Immunomodulatory Effect and Its Ability to Improve the Resistance against Helicobacter pylori Infection

The Exopolysaccharide of Lactobacillus fermentum UCO-979C Is Partially Involved in Its Immunomodulatory Effect and Its Ability to Improve the Resistance against Helicobacter pylori Infection

Can Probiotics, Particularly Limosilactobacillus fermentum UCO-979C and Lacticaseibacillus rhamnosus UCO-25A, Be Preventive Alternatives against SARS-CoV-2?

Lactobacillus strains vary in their ability to interact with human endometrial stromal cells

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