Characterization of the immune response to valve bioprostheses and its role in primary tissue failure

Human, Paul; Zilla, Peter

doi:10.1016/s0003-4975(01)02492-4

Cited by 95 publications

(73 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It might involve an immune-mediated reaction and an increased adsorption of proteins related to bone formation. 23,24 Another important mechanism of bioprosthetic degeneration is mechanical stress. The design of the valve and its stent are considered the 2 most important features to cope with mechanical stress.…”

Section: Potential Mechanisms Responsible For Svd In Dmmentioning

confidence: 99%

Type 2 Diabetes Mellitus Is Associated With Faster Degeneration of Bioprosthetic Valve

et al. 2012

View full text Add to dashboard Cite

resulted in 6184 patients (mean age 71.3Ϯ5.4 years, 60.1% male) being enrolled. Of these patients, 1731 (27.9%) had type 2 DM. The propensity score-matching algorithm successfully matched 1113 patients with type 2 DM with the same number of no-DM patients. The postmatching standard differences were less than 0.1 for each of the covariates, and 64.2% of DM patients were matched. The early (30 days) mortality rate was 7.8% (nϭ87) versus 2.9% (nϭ33) in patients with or without type 2 DM (PϽ0.001), respectively. Seven-year freedom from valve deterioration was significantly lower in patients with DM (73.2% [95% confidence interval, 61.6 -85.5] versus 95.4% [95% confidence interval, 83.9 -100], PϽ0.001). In Cox regression models with robust SEs that accounted for the clustering of matched pairs, DM was the strongest predictor of structural valve degeneration (hazard ratio 2.39 [95% confidence interval 2.28 -3.52]). When we allowed for interaction between type 2 DM and other key risk factors, DM remained a significant predictor beyond any potentially associated variable. Conclusions-Patients

show abstract

Section: Potential Mechanisms Responsible For Svd In Dmmentioning

confidence: 99%

Type 2 Diabetes Mellitus Is Associated With Faster Degeneration of Bioprosthetic Valve

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Abundant evidence demonstrates that both glutaraldehyde-fixed and unfixed BP xenografts elicit an immune response [1][2][3][4][5][6][7][8][27][28][29][30][31]. However, little is known about the nature and identity of BP proteins eliciting the immune response.…”

Section: Discussionmentioning

confidence: 99%

“…It is now clear that both humoral and cell-mediated immune responses to glutaraldehyde-fixed xenografts occur [1][2][3][4][5][6][7]. Mounting evidence implicates chronic antibody formation and immune rejection in bioprosthetic heart valve degeneration and calcification [2,3,6,7].…”

Section: Introductionmentioning

confidence: 99%

Immunoproteomic identification of bovine pericardium xenoantigens

et al. 2008

View full text Add to dashboard Cite

Bovine pericardium is an important biomaterial with current application in glutaraldehyde-fixed bioprosthetic heart valves and possible future application as an unfixed biological scaffold for tissue engineering. The importance of both humoral and cell-mediated rejection responses toward fixed and unfixed xenogeneic tissues has become increasingly apparent. However, the full scope and specific identities of bovine pericardium proteins that can elicit an immune response remain largely unknown. In this study, an immunoproteomic approach was used to survey bovine pericardium proteins for their ability to elicit a humoral immune response in rabbits. A two-stage protein extraction protocol was used to separate bovine pericardium proteins into water-and lipid-soluble fractions. Two-dimensional gel electrophoresis was performed to separate the proteins from each fraction. Western blots were generated from two-dimensional gels of both bovine pericardium protein fractions. These blots were probed with serum from rabbits immunized with bovine pericardium and a secondary antibody was used to assess for IgG positivity. Western blots were compared to duplicate two-dimensional gels and proteins in matched spots were identified by tandem mass spectrometry. Thirty-one putative protein antigens were identified, eight of which are known to be antigenic from previous studies. All of the putative antigens demonstrated progressive staining intensity with increasing days of post-exposure serum. Identified antigenic proteins represented a variety of functional and structural protein types, and included both cellular and matrix proteins. The results of this study have implications for the use of bovine pericardium as a biomaterial in bioprostheses and tissue engineering applications, as well as xenotransplantation in general.

show abstract

“…This causes the tissue to respond with cellular immune responses as the body acts to reject the tissue. Studies indicate that the level of cross-linking of the collagen fibres has the effect of reducing the antigenicity 8. However, the major challenge facing tissue valve use is still the link between immune response and calcific degeneration, and hence to be able to trace poorly masked immunogenicity 9…”

Section: Tissue Valvesmentioning

confidence: 99%

The design and development of a stented tissue mitral and aortic heart valve

Legg¹,

Mathews²,

Pelzer³

2012

Cardiovascular Journal of Africa

View full text Add to dashboard Cite

A study was conducted into the development of a mitral and aortic heart valve replacement that caters for patients having suffered valve damage due to stenosis or rheumatic fever. The appeal of the valve is that it is constituted from a solid frame housing pericardial tissue leaflets, and allows the patient freedom from post-operative blood-thinning medication. The valve is designed to appeal to patients in developing areas of the world, as it features a clip-in mechanism to secure the valve assembly into the sewing ring, which is stitched in independently of the frame and leaflets. Re-operative valve replacement would then be made possible when the pericardial leaflets began to calcify. Novel aspects of the design added value to the science of heart valve replacements, through the use of sintered chrome cobalt in the valve components, the insights gained into mechanical testing of pericardium, and the patient benefits offered by the complete design. Further work is planned to fatigue test the assembly, undergo animal trials and make the valve available for commercial use.

show abstract

Characterization of the immune response to valve bioprostheses and its role in primary tissue failure

Cited by 95 publications

References 8 publications

Type 2 Diabetes Mellitus Is Associated With Faster Degeneration of Bioprosthetic Valve

Type 2 Diabetes Mellitus Is Associated With Faster Degeneration of Bioprosthetic Valve

Immunoproteomic identification of bovine pericardium xenoantigens

The design and development of a stented tissue mitral and aortic heart valve

Contact Info

Product

Resources

About