Characterization of the
 parB
 -Like
 yyaA
 Gene of
 Bacillus subtilis

Sievers, Jörg; Raether, Brian; Perego, Marta; Errington, Jeff

doi:10.1128/jb.184.4.1102-1111.2002

Cited by 25 publications

(17 citation statements)

References 55 publications

(58 reference statements)

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“…Importantly, the ability of both Noc and SlmA to inhibit cell division is enhanced by their respective binding sequences (Wu et al , 2009; Cho et al , 2011; Tonthat et al , 2011). Consistent with its role in preventing division over the nucleoid, Noc overproduction causes a mild division block and strongly blocks the initiation of sporulation by preventing assembly of the asymmetric septum (Sievers et al , 2002; Wu & Errington, 2004). …”

Section: Introductionmentioning

confidence: 94%

Nucleoid occlusion protein N oc recruits DNA to the bacterial cell membrane

Errington

2015

The EMBO Journal

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127

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To proliferate efficiently, cells must co-ordinate division with chromosome segregation. In Bacillus subtilis, the nucleoid occlusion protein Noc binds to specific DNA sequences (NBSs) scattered around the chromosome and helps to protect genomic integrity by coupling the initiation of division to the progression of chromosome replication and segregation. However, how it inhibits division has remained unclear. Here, we demonstrate that Noc associates with the cell membrane via an N-terminal amphipathic helix, which is necessary for function. Importantly, the membrane-binding affinity of this helix is weak and requires the assembly of nucleoprotein complexes, thus establishing a mechanism for DNA-dependent activation of Noc. Furthermore, division inhibition by Noc requires recruitment of NBS DNA to the cell membrane and is dependent on its ability to bind DNA and membrane simultaneously. Indeed, Noc production in a heterologous system is sufficient for recruitment of chromosomal DNA to the membrane. Our results suggest a simple model in which the formation of large membrane-associated nucleoprotein complexes physically occludes assembly of the division machinery.

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Section: Introductionmentioning

confidence: 94%

Nucleoid occlusion protein N oc recruits DNA to the bacterial cell membrane

Errington

2015

The EMBO Journal

Self Cite

127

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“…The nucleoid occlusion (NO) system prevents premature Z-ring formation over the nucleoid until most of the chromosomal DNA has been segregated. Spo0J together with its paralog, Noc [187][188][189], block premature Z-ring assembly, preventing chromosome guillotining in the dividing cells. The deletion of either noc (initially named yyaA) or spo0J results in aberrant cell division, and the deletion of both noc and spo0J has a synergistic effect, potentiating the aberrations [190].…”

Section: Involvement Of Parb In the Nucleoid Occlusionmentioning

confidence: 99%

Rules and Exceptions: The Role of Chromosomal ParB in DNA Segregation and Other Cellular Processes

et al. 2020

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The segregation of newly replicated chromosomes in bacterial cells is a highly coordinated spatiotemporal process. In the majority of bacterial species, a tripartite ParAB-parS system, composed of an ATPase (ParA), a DNA-binding protein (ParB), and its target(s) parS sequence(s), facilitates the initial steps of chromosome partitioning. ParB nucleates around parS(s) located in the vicinity of newly replicated oriCs to form large nucleoprotein complexes, which are subsequently relocated by ParA to distal cellular compartments. In this review, we describe the role of ParB in various processes within bacterial cells, pointing out interspecies differences. We outline recent progress in understanding the ParB nucleoprotein complex formation and its role in DNA segregation, including ori positioning and anchoring, DNA condensation, and loading of the structural maintenance of chromosome (SMC) proteins. The auxiliary roles of ParBs in the control of chromosome replication initiation and cell division, as well as the regulation of gene expression, are discussed. Moreover, we catalog ParB interacting proteins. Overall, this work highlights how different bacterial species adapt the DNA partitioning ParAB-parS system to meet their specific requirements.

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“…In the case of Noc, this would suggest that it somehow contributes to chromosome segregation. Given the homology of Noc to Spo0J (36% sequence identity (Sievers et al, 2002)), this may not be so surprising. In fact, previous work indicates that Noc may reside in a complex with SMC (Gruber and Errington, 2009).…”

Section: Spo0j and Noc Are Required To Fine-tune Thetiming Of Z Ring mentioning

confidence: 99%

The ParB homologs, Spo0J and Noc, together prevent premature midcell Z ring assembly when the early stages of replication are blocked in Bacillus subtilis

Hajduk

Mann

Rodrigues

et al. 2019

Molecular Microbiology

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SummaryPrecise cell division in coordination with DNA replication and segregation is of utmost importance for all organisms. The earliest stage of cell division is the assembly of a division protein FtsZ into a ring, known as the Z ring, at midcell. What still eludes us, however, is how bacteria precisely position the Z ring at midcell. Work in B. subtilis over the last two decades has identified a link between the early stages of DNA replication and cell division. A recent model proposed that the progression of the early stages of DNA replication leads to an increased ability for the Z ring to form at midcell. This model arose through studies examining Z ring position in mutants blocked at different steps of the early stages of DNA replication. Here, we show that this model is unlikely to be correct and the mutants previously studied generate nucleoids with different capacity for blocking midcell Z ring assembly. Importantly, our data suggest that two proteins of the widespread ParB family, Noc and Spo0J are required to prevent Z ring assembly over the bacterial nucleoid and help fine tune the assembly of the Z ring at midcell during the cell cycle.

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Characterization of the parB -Like yyaA Gene of Bacillus subtilis

Cited by 25 publications

References 55 publications

Nucleoid occlusion protein N oc recruits DNA to the bacterial cell membrane

Nucleoid occlusion protein N oc recruits DNA to the bacterial cell membrane

Rules and Exceptions: The Role of Chromosomal ParB in DNA Segregation and Other Cellular Processes

The ParB homologs, Spo0J and Noc, together prevent premature midcell Z ring assembly when the early stages of replication are blocked in Bacillus subtilis

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