Characterization of theMycobacterium tuberculosisregion containing thempt83andmpt70genes

Juárez, Marı́a Dolores; Torres, Ascención; Espitia, Clara

doi:10.1111/j.1574-6968.2001.tb10826.x

Cited by 19 publications

(1 citation statement)

References 23 publications

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“…(Supplementary Table 9). Other antigens exhibiting parallel nonsynonymous changes by different sublineages include known immunodominant, secreted antigens such as Mpb6464, MPT3265 and MPT7066 and three latency-associated antigens (Rv1733c67, Rv3034c, Rv262868, Supplementary Table 9). …”

Section: Resultsmentioning

confidence: 99%

Mycobacterium tuberculosis lineage 4 comprises globally distributed and geographically restricted sublineages

et al. 2016

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Generalist and specialist species differ in the breadth of their ecological niche. Little is known about the niche width of obligate human pathogens. Here we analyzed a global collection of Mycobacterium tuberculosis Lineage 4 clinical isolates, the most geographically widespread cause of human tuberculosis. We show that Lineage 4 comprises globally distributed and geographically restricted sublineages, suggesting a distinction between generalists and specialists. Population genomic analyses showed that while the majority of human T cell epitopes were conserved in all sublineages, the proportion of variable epitopes was higher in generalists. Our data further support a European origin for the most common generalist sublineage. Hence, the global success of Lineage 4 reflects distinct strategies adopted by different sublineages and the influence of human migration.

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Section: Resultsmentioning

confidence: 99%

Mycobacterium tuberculosis lineage 4 comprises globally distributed and geographically restricted sublineages

et al. 2016

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Structural Basis and Kinetics of DsbD-Dependent Cytochrome c Maturation

Stirnimann

Rozhkova²,

Grauschopf³

et al. 2005

Structure

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DsbD from Escherichia coli transports two electrons from cytoplasmic thioredoxin to the periplasmic substrate proteins DsbC, DsbG and CcmG. DsbD consists of an N-terminal periplasmic domain (nDsbD), a C-terminal periplasmic domain, and a central transmembrane domain. Each domain possesses two cysteines required for electron transport. Herein, we demonstrate fast (3.9 x 10(5) M(-1)s(-1)) and direct disulfide exchange between nDsbD and CcmG, a highly specific disulfide reductase essential for cytochrome c maturation. We determined the crystal structure of the disulfide-linked complex between nDsbD and the soluble part of CcmG at 1.94 A resolution. In contrast to the other two known complexes of nDsbD with target proteins, the N-terminal segment of nDsbD contributes to specific recognition of CcmG. This and other features, like the possibility of using an additional interaction surface, constitute the structural basis for the adaptability of nDsbD to different protein substrates.

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Differential gene expression between Mycobacterium bovis and Mycobacterium tuberculosis

Rehren

Walters²,

Fontán³

et al. 2007

Tuberculosis

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The high sequence identity among the Mycobacterium bovis and Mycobacterium tuberculosis genomes contrasts with the physiological differences reported between these pathogens, suggesting that variations in gene expression may be involved. In this study, microarray hybridization was used to compare the total transcriptome of M. bovis and M. tuberculosis, during the exponential phase of growth. Differential expression was detected in 258 genes, representing a 6% of the total genome. Variable genes were grouped according to functional categories. The main variations were found in genes encoding proteins involved in intermediary metabolism and respiration, cell wall processes, and hypothetical proteins. It is noteworthy that, compared to M. tuberculosis, the expression of a higher number of transcriptional regulators were detected in M. bovis. Likewise, in M. tuberculosis we found a higher expression of the PE/PPE genes, some of which code for cell wall related proteins. Also, in both pathogens we detected the expression of a number of genes not annotated in the M. tuberculosis H37Rv or M. bovis 2122 genomes, but annotated in the M. tuberculosis CDC1551 genome. Our results provide new evidence concerning differences in gene expression between both pathogens, and confirm previous hypotheses inferred from genome comparisons and proteome analysis. This study may shed some new light on our understanding of the mechanisms relating to differences in gene expression and pathogenicity in mycobacteria.

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Characterization of theMycobacterium tuberculosisregion containing thempt83andmpt70genes

Cited by 19 publications

References 23 publications

Mycobacterium tuberculosis lineage 4 comprises globally distributed and geographically restricted sublineages

Mycobacterium tuberculosis lineage 4 comprises globally distributed and geographically restricted sublineages

Structural Basis and Kinetics of DsbD-Dependent Cytochrome c Maturation

Differential gene expression between Mycobacterium bovis and Mycobacterium tuberculosis

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