Characterization of the cytochrome b (cyt b) gene from Monilinia species causing brown rot of stone and pome fruit and its significance in the development of QoI resistance

Hily, Jean-Michel; Singer, Stacy D.; Villani, Sara M.; Cox, Kerik D.

doi:10.1002/ps.2074

Cited by 51 publications

(40 citation statements)

References 65 publications

(112 reference statements)

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“…The Cyt b genes were previously isolated from peach brown rot fungi M. fructicola , M. fructigena , M. laxa , M. yunnanensis and M. mumecola [31]–[33]. In the present study, the Cyt b gene was isolated from the rice false smut fungus Ustilaginoidea virens .…”

Section: Methodsmentioning

confidence: 80%

“…In recent studies on peach brown rot fungi Monilinia spp. (anamorphs in Monilia ), Cyt b genes, the targets of the Quinone outside inhibitor fungicides (QoIs), were isolated and analyzed [31]–[33]. The Cyt b genes in M. fructicola , M. laxa , M. fructigena and M. yunnanensis have seven, whereas the Cyt b gene in M. mumecola has six large (>1 kb) introns.…”

Section: Introductionmentioning

confidence: 99%

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Frequent Gain and Loss of Introns in Fungal Cytochrome b Genes

Yin

Wang

et al. 2012

PLoS ONE

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In this study, all available cytochrome b (Cyt b) genes from the GOBASE database were compiled and the evolutionary dynamics of the Cyt b gene introns was assessed. Cyt b gene introns were frequently present in the fungal kingdom and some lower plants, but generally absent or rare in Chromista, Protozoa, and Animalia. Fungal Cyt b introns were found at 35 positions in Cyt b genes and the number of introns varied at individual positions from a single representative to 32 different introns at position 131, showing a wide and patchy distribution. Many homologous introns were present at the same position in distantly related species but absent in closely related species, suggesting that introns of the Cyt b genes were frequently lost. On the other hand, highly similar intron sequences were observed in some distantly related species rather than in closely related species, suggesting that these introns were gained independently, likely through lateral transfers. The intron loss-and-gain events could be mediated by transpositions that might have occurred between nuclear and mitochondria. Southern hybridization analysis confirmed that some introns contained repetitive sequences and might be transposable elements. An intron gain in Botryotinia fuckeliana prevented the development of QoI fungicide resistance, suggesting that intron loss-and-gain events were not necessarily beneficial to their host organisms.

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Section: Methodsmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Frequent Gain and Loss of Introns in Fungal Cytochrome b Genes

Yin

Wang

et al. 2012

PLoS ONE

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“…The presence of this fungal species is associated with the degradation of pine needles 20 and may reflect the forested environment of Washington State as these homes were located 10 to 40 miles from heavily forested regions. The Yakima Valley is one of the largest fruit tree growing areas in the US which may explain the occurrence of Monilinia laxa , a pathogen of peach trees 21 or Oidium aloysiae , from the powdery mildew family of fungal pathogens. 22 The somewhat surprising finding of relative high concentrations of Juncigena adarca , typically associated with salt marsh plants, may be explained by the Westerly's and the winds that predominate during the winter months and which are known to bring a maritime presence to the valley from the coastal area.…”

Section: Discussionmentioning

confidence: 99%

Utilizing pyrosequencing and quantitative PCR to characterize fungal populations among house dust samples

et al. 2012

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Molecular techniques are replacing culturing and counting methods in quantifying indoor fungal contamination. Pyrosequencing offers the possibility of identifying unexpected indoor fungi. In this study, 50 house dust samples were collected from homes in the Yakima Valley, WA. Each sample was analyzed by quantitative PCR (QPCR) for 36 common fungi and by fungal tag-encoded flexible (FLX) amplicon pyrosequencing (fTEFAP) for these and additional fungi. Only 24 of the samples yielded amplified results using fTEFAP but QPCR successfully amplified all 50 samples. Over 450 fungal species were detected by fTEFAP but most were rare. Twenty-two fungi were found by fTEFAP to occur with at least an average of ≥ 0.5% relative occurrence. Many of these fungi seem to be associated with plants, soil or human skin. Combining fTEFAP and QPCR can enhance studies of fungal contamination in homes.

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“…Similarly, the methods developed by Ioos and Frey [91] and Ma et al [97,100] did not discriminate between M. mumecola and M. laxa. In addition, the method developed by Hily et al [99] did not differentiate M. mumecola from M. fructicola, and the methods of Miessner and Stammler [98] and Hily et al [99] also could not discriminate between M. yunnanensis and M. mumecola. Moreover, some investigators [96] in their study of RT-PCR detection of latent Monilinia spp.…”

Section: Molecular Methodsmentioning

confidence: 96%

“…The characterization methods of Ioos and Frey [91], Miessner and Stammler [98], and Hily et al [99] reliably differentiated three species of Monilinia (M. fructigena, M. fructicola, and M. laxa), but were practically unable to distinguish M. fructigena from M. yunnanensis. Similarly, the methods developed by Ioos and Frey [91] and Ma et al [97,100] did not discriminate between M. mumecola and M. laxa.…”

Section: Molecular Methodsmentioning

confidence: 99%

Peach Brown Rot: Still in Search of an Ideal Management Option

2018

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Abstract:The peach is one of the most important global tree crops within the economically important Rosaceae family. The crop is threatened by numerous pests and diseases, especially fungal pathogens, in the field, in transit, and in the store. More than 50% of the global post-harvest loss has been ascribed to brown rot disease, especially in peach late-ripening varieties. In recent years, the disease has been so manifest in the orchards that some stone fruits were abandoned before harvest. In Spain, particularly, the disease has been associated with well over 60% of fruit loss after harvest. The most common management options available for the control of this disease involve agronomical, chemical, biological, and physical approaches. However, the effects of biochemical fungicides (biological and conventional fungicides), on the environment, human health, and strain fungicide resistance, tend to revise these control strategies. This review aims to comprehensively compile the information currently available on the species of the fungus Monilinia, which causes brown rot in peach, and the available options to control the disease. The breeding for brown rot-resistant varieties remains an ideal management option for brown rot disease control, considering the uniqueness of its sustainability in the chain of crop production.

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Characterization of the cytochrome b (cyt b) gene from Monilinia species causing brown rot of stone and pome fruit and its significance in the development of QoI resistance

Abstract: Results suggest that resistance to QoI fungicides based on the G143A mutation is not likely to occur in M. fructicola or M. laxa. Conversely, M. fructigena may be at higher risk for developing QoI resistance owing to the absence of a G143-associated intron.

Cited by 51 publications

References 65 publications

Frequent Gain and Loss of Introns in Fungal Cytochrome b Genes

Frequent Gain and Loss of Introns in Fungal Cytochrome b Genes

Utilizing pyrosequencing and quantitative PCR to characterize fungal populations among house dust samples

Peach Brown Rot: Still in Search of an Ideal Management Option

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