Characterization of the humanMDR1 gene

Bodor, Miklós; Kelley, Edward J.; Ho, Rodney J. Y.

doi:10.1208/aapsj070101

Cited by 81 publications

(53 citation statements)

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“…Its transcription gives rise to 4 mRNA variants, translated, for three of them, into a single protein of 1280 amino acids. 8 At the protein level (Figure 1), MDR1 is formed by a single 170 kDa monomer of 12 transmembrane domains (TMs), glycosylated on its first extracellular loop (between TM1 and 2). 1,2 Two distinct cytoplasmic ATP binding sites, respectively located on the loop between the TMs 6 and 7 and after the TM 12, work alternatively and cooperatively to efflux the substrate.…”

Section: Introductionmentioning

confidence: 99%

MDR1 in immunity: friend or foe?

et al. 2018

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MDR1 is an ATP-dependent transmembrane transporter primarily studied for its role in the detoxification of tissues and for its implication in resistance of tumor cells to chemotherapy treatment. Several studies also report on its expression on immune cells where it plays a protective role from xenobiotics and toxins. This review provides an overview of what is known on MDR1 expression in immune cells in human, and its implications in different pathologies and their treatment options.

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Section: Introductionmentioning

confidence: 99%

MDR1 in immunity: friend or foe?

et al. 2018

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“…Also called ABCB1, the MDR1 gene is located in chromosome 7q21.1 (5) and comprises a promoter region of 29 exons, with a total of 209kb (3,6) . MDR1 codes for the P-glycoprotein (PgP) 1, a member of the superfamily of ABC transporters (ATP-binding cassette), which includes proteins that transport a wide variety of substrates (8,9) .…”

Section: Frequency Of the Mdr1 Gene Polymorphism Rs1045642 (C3435t) Imentioning

confidence: 99%

Frequency of the Mdr1 Gene Polymorphism Rs1045642 (C3435t) in HCV-Hiv Co-Infected Patients

Marasca

Machado

Filho

et al. 2016

Arq. Gastroenterol.

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-Background -Due to the high prevalence of co-infection by hepatitis C virus (HCV) and human immunodeficiency virus (HIV) and the severity of these infections, the understanding of the biological mechanisms involved in these processes, including viral behavior and host genetic profile, is of great importance for patient treatment and for public health policies. Some single nucleotide polymorphisms (SNPs) in the human genome, such as the SNP rs1045642 (C3435T) in the MDR1 gene, have been reported to be associated to the sustained virological response (SVR) to HCV treatment in HCV-HIV co-infected patients. Objective -The present study analyzes the MDR1 gene C3435T polymorphism in HCV-HIV co-infected patients.Methods -A total of 99 HCV-HIV patients were included in the study. The DNA was extracted from blood samples, and the SNP rs1045642 was assessed by Real Time PCR (qPCR). Risk factors for acquiring the virus and the SVR after HCV treatment with pegylated interferon-alpha and ribavirin were also analyzed. Results -Among the patients, 54 (54.5%) were male and 45 (45.5%) were female. The average age was 46.1±9.8 years. The SVR after HCV treatment was 40%. The frequencies of MDR1 genotypes CC, CT and TT were 28.3%, 47.5% and 24.2%, respectively. Allele frequencies were 52% for the C allele and 48% for the T allele. No association was found for SNP rs1045642 (C3435T) regarding response to treatment (P=0.308). Conclusion -In this study, the C3435T polymorphism in the MDR1 gene appears not to be associated with SVR in HCV-HIV co-infected individuals. HEADINGS -Hepacivirus. HIV. Single nucleotide polymorphism. P-glycoprotein. Coinfection. Interferon-alpha. Ribavirin.Declared conflict of interest of all authors: none Disclosure of funding: no funding received Financial support: Marasca GS and Souza ACS hold a fellowship from CAPES (Ministry of Education, Brazil).

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“…This transmembrane protein has a molecular weight of 170 kd, 1280 amino acids, is encoded by the gene MDR-1 and depends on energy coming from the metabolism of adenosine triphosphate (ATP) (Sauna et al, 2001). The MDR -1 gene in humans is located on the long arm of chromosome 7 (7 q 21) consisting of a central promoter region and 29 exons ranging from 6.3 to 210 kilobases (Bodor et al, 2005). Pgp is the most investigated of a superfamily called ATP -binding cassette transporters, or ABC multidrug transporters.…”

Section: P-glycoproteinmentioning

confidence: 99%