1998
DOI: 10.1007/s004240050676
|View full text |Cite
|
Sign up to set email alerts
|

Characterization of the human type II Na/P i -cotransporter promoter

Abstract: The type II Na/Pi-cotransporter is expressed preferentially in renal proximal tubular epithelial cells. Comparison of the 5′ flanking region of the human NPT-2 gene with the opossum cell line (OK cell) and the murine Npt2 promoters revealed two conserved regions, one representing a putative C/EBP alpha site, the other a consensus TATA-box. In contrast to the OK cell and murine Npt-2 gene, the human exon 1 is flanked by two Alu-repeats, a short 90-bp inverted Alu element which is located within the promoter reg… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
17
0

Year Published

2000
2000
2020
2020

Publication Types

Select...
8
2

Relationship

0
10

Authors

Journals

citations
Cited by 19 publications
(17 citation statements)
references
References 23 publications
0
17
0
Order By: Relevance
“…For the murine gene, two transcription initiation and two polyadenylation sites have been identified. The NPT2 promoter was functional in cells from renal proximal origin (OK), but not from distal tubules (MDCK) [17]. The promoter activity is regulated by CO 2 /HCO 3 2– and vitamin D 3 [18, 19]; transcriptional regulation has also been proposed for thyroid hormone (T 3 ) [20].…”
Section: Molecular Characterization Of the Renal-specific Type-iia Namentioning
confidence: 99%
“…For the murine gene, two transcription initiation and two polyadenylation sites have been identified. The NPT2 promoter was functional in cells from renal proximal origin (OK), but not from distal tubules (MDCK) [17]. The promoter activity is regulated by CO 2 /HCO 3 2– and vitamin D 3 [18, 19]; transcriptional regulation has also been proposed for thyroid hormone (T 3 ) [20].…”
Section: Molecular Characterization Of the Renal-specific Type-iia Namentioning
confidence: 99%
“…NaPi-IIa also shows a high degree of homology to another transporter, NaPi-IIb, at the level of primary structure (ϳ70% similarity), but these transporters show different expression patterns in vivo (9,15). NaPi-IIa is expressed on the apical membrane of renal proximal tubules, and NaPi-IIb is expressed in the intestine and lung epithelia.…”
mentioning
confidence: 99%
“…The 5'-flanking regions of the rat (~4kb rNpt2a), human (~2.4kb hNpt2a), and opossum (~3.7kb oNpt2a) genes were active in reporter gene assays only in the OK cells but not HeLa, NIH-3T3, or HEK293 cells [6][7][8][9]. 5'-deletion analyses identified a region within 250bp upstream of the transcription start site as sufficient to drive cell-specific expression of the human and opossum promoters [6,10]. A transgenic mouse harboring a rat Npt2a promoter-driven LacZ gene showed localized renal cortical-specific expression in the kidney and no other tissue, confirming that 4.7kb of the rat 5'-flanking region is sufficient to control tissue-specific expression in vivo [11].…”
Section: Introductionmentioning
confidence: 99%