Characterization of the Human C‐
            <i>myc</i>
            Protein using Antibodies Prepared Against Synthetic Peptides

Gi, Evan; Dc, Hancock; Littlewood, Trevor D.; Cd, Pauza

doi:10.1002/9780470513286.ch14

Cited by 5 publications

(2 citation statements)

References 24 publications

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“…Second, the antibody depletion mechanism that is used for the elimination of TCR-redirected T cells is specific. C-myc is a nuclear protein and is not expressed on the surface of normal cells (33). Hence, application of a myc-specific antibody is not toxic for other cells.…”

Section: Discussionmentioning

confidence: 99%

“…The OT-I TCR (v␣2/v␤5, recognizing the ovalbumin257-264 peptide SIINFEKL) was isolated from the cDNA of splenocytes of OT-I/Rag-1 Ϫ/Ϫ mice (36) and cloned into the MP71 retroviral vector (37) via NotI and BsrGI restriction sites. The construction of P14 TCR (v␣2/v␤8, recognizing the lymphocytic choriomeningitis virus glycoprotein [33][34][35][36][37][38][39][40][41] peptide KAVYNFATM) and gp100 TCR (v␣13/v␤8, recognizing the human gp100 209 -217 peptide IMDQVPFSV) vectors has been described previously (8,38,39). In bicistronic constructs, the ␣-and ␤-chains, were linked via the P2A peptide (40) to obtain MP71-TCR␣-P2A-TCR␤.…”

Section: Methodsmentioning

confidence: 99%

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A safeguard eliminates T cell receptor gene-modified autoreactive T cells after adoptive transfer

Kieback

Charo

Sommermeyer

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

A safeguard eliminates T cell receptor gene-modified autoreactive T cells after adoptive transfer

Kieback

Charo

Sommermeyer

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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Raising Antibodies by Coupling Peptides to PPD and Immunizing BCG‐Sensitized Animals

Lachmann

Strangeways

Vyakarnam

et al. 2007

Novartis Foundation Symposia

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Destruction of Xenopus cyclins A and B2, but not B1, requires binding to p34cdc2.

Stewart¹,

Kobayashi²,

Harrison³

et al. 1994

The EMBO Journal

110

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The specific and rapid destruction of cyclins A and B during mitosis is their most remarkable property. A short peptide motif of approximately 10 amino acids near the N‐terminus, known as the destruction box, is absolutely required for programmed proteolysis. In this paper we show that although the destruction box is necessary for the degradation of cyclin A, it is not sufficient. Mutant versions of cyclin A that cannot form complexes with p34cdc2 are stable, which we interpret to mean that this cyclin must bind to p34cdc2 in order to undergo programmed proteolysis. Thus, N‐terminal fragments of cyclin A containing little more than the destruction box and its surroundings are indestructible. p34cdc2 binding also appears to be required for the destruction of cyclin B2. In contrast, cyclin B1 does not require p34cdc2 binding for specific proteolysis. The systems for the proteolysis of cyclins A, B1 and B2 thus appear to show important differences in the way they recognize their substrates.

show abstract

Characterization of the Human C‐ myc Protein using Antibodies Prepared Against Synthetic Peptides

Cited by 5 publications

References 24 publications

A safeguard eliminates T cell receptor gene-modified autoreactive T cells after adoptive transfer

A safeguard eliminates T cell receptor gene-modified autoreactive T cells after adoptive transfer

Raising Antibodies by Coupling Peptides to PPD and Immunizing BCG‐Sensitized Animals

Destruction of Xenopus cyclins A and B2, but not B1, requires binding to p34cdc2.

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