Characterization of the gut bacterial community of the Japanese sea cucumber Apostichopus japonicus

Богатыренко, Е. А.; Бузолева, Л. С.

doi:10.1134/s0026261716010033

Cited by 20 publications

(18 citation statements)

References 20 publications

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“…With the development and application of high‐throughput sequencing technology, an increasing number of studies have been extensively carried out on microbial communities in multi‐media environments, such as human body (feces, gut, oral cavity, nasal cavity, skin and genital tract) (Spor, Koren & Ley, ), soil (Rondon et al., ), ocean (Haq & Elsas, ) and other aquatic ecosystems. Currently, a few studies have focused on the structure of bacterial community in the gut of sea cucumber (Bogatyrenko & Buzoleva, ; Gao, Tan, Sun & Yan, ; Sha et al., ). However, the use of metagenomic sequencing technology has been scarcely reported.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the bacterial community in different parts of the gut of sea cucumber (Apostichopus Japonicus ) and its variation during gut regeneration

Luo

Zhao

et al. 2018

Aquac Res

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Bacterial community plays an important role in keeping the health status of the host. A study on the characteristics of gut bacterial community of sea cucumber (Apostichopus japonicus) not only helps improve the current aquaculture model, but promotes healthy and rapid development of the aquaculture industry as well. Therefore, taking pond‐cultured sea cucumber (A. japonicus) as the studying object, the metagenomic and 16S rRNA sequencing technology were used in this study to explore the characteristics of bacterial community in different parts of the gut of sea cucumber, as well as during gut regeneration after evisceration. The results showed that the compositions of bacterial community are different in varying parts of the gut of sea cucumber (A. japonicus). Specifically, bacterial community in the midgut and hindgut are highly similar, showing significantly diversified bacterial species compared to the foregut. GO annotation indicated that the foregut is associated with richer catalytic activity and binding than the midgut and the hindgut. According to the KEGG annotation, metabolism‐related genes are mainly concentrated in the foregut, while genes related to signal transduction and the immune system are mostly annotated in the midgut and hindgut. During the gut regeneration stage, the structure of bacterial community varied greatly in different stage of the regeneration stage, with significant differences between the earlier and later stage. The dominant bacteria in the earlier stage is Rubritalea, and that in the later stage is Arcobacter. Besides, there were Loktanella, Thalassobacter and Phaeobacter in the gut throughout the entire regeneration stage. Cupriavidus, Hellea, HTCC2207, Methylophaga, Methylotenera, Stenotrophomonas and Tenacibaculum were only present in the earlier stage, and gradually disappeared in the later stage due to improving gut functions. The abundance and diversity of bacteria in the gut were higher in the earlier regeneration stage than that in the later stage, with a peak between the 15th and 25th day of the regeneration stage. At 45th day, the abundance and diversity became stable.

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Section: Introductionmentioning

confidence: 99%

Characterization of the bacterial community in different parts of the gut of sea cucumber (Apostichopus Japonicus ) and its variation during gut regeneration

Luo

Zhao

et al. 2018

Aquac Res

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“…The culture‐dependent approach applied in this study resulted in the isolation of bacterial strains belonging to 13 genera from 10 families in three phyla (Firmicutes, Actinobacteria and Proteobacteria). Of these, nine genera ( Bacillus , Halobacillus , Micrococcus , Nocardiopsis , Pseudomonas , Shewanella , Streptomyces , Vibrio and Virgibacillus ) were previously isolated from A. japonicus or other types of sea cucumber (Enomoto et al ; Zhang et al ; Bogatyrenko and Buzoleva ). However, this is the first time that four genera in the phylum Actinobacteria, including Brachybacterium , Cellulosimicrobium , Glutamicibacter and Microbacterium , have been isolated from A. japonicus .…”

Section: Discussionmentioning

confidence: 99%

Biosynthetic potential of culturable bacteria associated with Apostichopus japonicus

Chen

et al. 2019

J Appl Microbiol

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Aims Isolating culturable bacteria associated with sea cucumber (Apostichopus japonicus) and investigating their potential bioactivities are important approaches to discover natural marine products. Methods and Results A total of 161 isolates were obtained from sea cucumber collected along the Weihai coast of the North Yellow Sea, China. Identification and phylogenetic analysis of 61 isolates were conducted by 16S rRNA gene sequencing. The isolates belonged to 13 genera from 10 families in three phyla, including Firmicutes, Actinobacteria and Proteobacteria. The antimicrobial activities of all strains were determined using six indicator strains. Of the 161 isolates, 93 showed antibacterial activities against at least one of the indicator strains. The 26 strains with the strongest inhibitory effects were selected for screening the biosynthetic gene clusters of polyketide synthase (PKS‐I, ‐II) and nonribosomal peptide synthetase (NRPS). Phylogenetic trees based on the amino acid sequences of the PKS or NRPS genes were constructed. Eleven strains with PKS genes and four strains with NRPS genes were detected. Conclusions The data reveal the diversity of culturable bacteria associated with A. japonicus. Most strains showed broad‐spectrum antimicrobial activities, and some strains with antimicrobial activities possessed PKS and NRPS genes. Significance and Impact of the Study The results suggest that culturable bacteria associated with A. japonicus may act as a promising source of bioactive substances.

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“…For instance, the study on microbiota in sea cucumber Apostichopus japonicus (Selenka, 1867) and in the bot tom sediments on the surface of which this species dwells revealed considerable similarity in the micro bial composition, e.g., the prevalence of the species of gg. Pseudomonas and Bacillus [1].…”

Section: Discussionmentioning

confidence: 99%

The in vitro effect of pH on glycosidase activity in potential preys of planktivorous and benthivorous fish

2015

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It is shown that the level of total amylolytic activity (TAA) in the whole organism of potential preys (belonging to various taxonomic groups) of planktivorous and benthivorous fishes, as well as in the associated microbiota, depends largely on pH values in a medium. Maximal values of TAA in all species of invertebrates and in the micro flora isolated from their bodies are observed at pH 7.0; minimal ones are found at pH 10.0. The microbiota of Dreissena and of crustacean plankton may efficiently depolymerize polysaccharides within the zone of acid pH values.

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Characterization of the gut bacterial community of the Japanese sea cucumber Apostichopus japonicus

Cited by 20 publications

References 20 publications

Characterization of the bacterial community in different parts of the gut of sea cucumber (Apostichopus Japonicus ) and its variation during gut regeneration

Characterization of the bacterial community in different parts of the gut of sea cucumber (Apostichopus Japonicus ) and its variation during gut regeneration

Biosynthetic potential of culturable bacteria associated with Apostichopus japonicus

The in vitro effect of pH on glycosidase activity in potential preys of planktivorous and benthivorous fish

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