Characterization of the Genetic Diversity of T4-like Bacteriophages in Benthic Biofilms of Lake Baikal

Potapov, Sergey; Krasnopeev, Andrey; Тихонова, И. В.; Galachyants, A. D.; Podlesnaya, G. V.; Ханаев, И. В.; Белых, О. И.

doi:10.26516/2073-3372.2018.25.15

Cited by 4 publications

(3 citation statements)

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“…Geological, geographical and hydrological characteristics of the lake reflect its uniqueness and high endemism of aquatic organisms [28]. Although some studies characterised viruses of Lake Baikal [25,26,29,30], their genetic diversity remains insufficiently investigated.…”

Section: Introductionmentioning

confidence: 99%

Metagenomic Analysis of Virioplankton from the Pelagic Zone of Lake Baikal

Potapov

Тихонова

Krasnopeev

et al. 2019

Viruses

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This study describes two viral communities from the world’s oldest lake, Lake Baikal. For the analysis, we chose under-ice and late spring periods of the year as the most productive for Lake Baikal. These periods show the maximum seasonal biomass of phytoplankton and bacterioplankton, which are targets for viruses, including bacteriophages. At that time, the main group of viruses were tailed bacteriophages of the order Caudovirales that belong to the families Myoviridae, Siphoviridae and Podoviridae. Annotation of functional genes revealed that during the under-ice period, the “Phages, Prophages, Transposable Elements and Plasmids” (27.4%) category represented the bulk of the virome. In the late spring period, it comprised 9.6% of the virome. We assembled contigs by two methods: Separately assembled in each virome or cross-assembled. A comparative analysis of the Baikal viromes with other aquatic environments indicated a distribution pattern by soil, marine and freshwater groups. Viromes of lakes Baikal, Michigan, Erie and Ontario form the joint World’s Largest Lakes clade.

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Section: Introductionmentioning

confidence: 99%

Metagenomic Analysis of Virioplankton from the Pelagic Zone of Lake Baikal

Potapov

Тихонова

Krasnopeev

et al. 2019

Viruses

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“…UniFrac analysis showed that uncultured T4-like viruses from eutrophic region of Lake Baikal tended to cluster with those from the distant lake of the same trophic status. Authors supposed this phage to attack baikalian cyanobacteria and, thus, to play an important role in the lake ecosystem [14,15]. The same research group continued sampling in Southern, Central and Northern Baikal from depth 0, 5, 15, 25, 50 m. As result the high diversity of the family Myoviridae cyanophages in Lake Baikal was recorded analyzing capsid assembly protein genes g20.…”

Section: Results Of Virus Researchesmentioning

confidence: 99%

Viruses in the Lake Baikal Ecosystem

Ea¹

2018

VIJ

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The most voluminous and oldest fresh lake Baikal is inhabited by diverse and numerous representatives of viral life. Its pelagic waters contain phages both in surface layer, as well as in water mass itself till the maximal depths in all three through (Southern, Central, Northern Baikal). The most of baikalian phages belong to Caudovirales. Siphoviridae, Podoviridae, and especially Myoviridae practically dominate both virioplankton and natural biofilms of the lake. The number of phages is rather significant-up to 107 ml-1 in upper layers (0-25 m) of water during seasons of mass development (March-April, August-October). Seasonal dynamics of number of phages follows the number dynamics of heterotrophic bacteria and phototrophic cyanobacteria. Lysis of significant share of bacteria provides phages with important role in bacterial loop of trophic net of the lake.

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“…Since 2010, viruses in Lake Baikal have continued to be studied mainly using molecular techniques (targeted sequencing and shotgun), first by analysing signature genes of DNA viral communities [ 16 , 17 , 18 ] and later by high-throughput sequencing for metagenomic analysis of DNA viromes and genome assembly of isolated DNA strains [ 19 , 20 , 21 , 22 , 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

The Viral Fraction Metatranscriptomes of Lake Baikal

et al. 2022

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This article characterises viral fraction metatranscriptomes (smaller than 0.2 µm) from the pelagic zone of oligotrophic Lake Baikal (Russia). The study revealed the dominance of transcripts of DNA viruses: bacteriophages and algal viruses. We identified transcripts similar to Pithovirus sibericum, a nucleocytoplasmic large DNA virus (NCLDV) isolated from the permafrost region of Eastern Siberia. Among the families detected were RNA viruses assigned to Retroviridae, Metaviridae, Potyviridae, Astroviridae, and Closteroviridae. Using the PHROG, SEED subsystems databases, and the VOGDB, we indicated that the bulk of transcripts belong to the functional replication of viruses. In a comparative unweighted pair group method with arithmetic mean (UPGMA) analysis, the transcripts from Lake Baikal formed a separate cluster included in the clade with transcripts from other freshwater lakes, as well as marine and oceanic waters, while there was no separation based on the trophic state of the water bodies, the size of the plankton fraction, or salinity.

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Characterization of the Genetic Diversity of T4-like Bacteriophages in Benthic Biofilms of Lake Baikal

Cited by 4 publications

References 0 publications

Metagenomic Analysis of Virioplankton from the Pelagic Zone of Lake Baikal

Metagenomic Analysis of Virioplankton from the Pelagic Zone of Lake Baikal

Viruses in the Lake Baikal Ecosystem

The Viral Fraction Metatranscriptomes of Lake Baikal

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