Characterization of the gene encoding glycoprotein C of duck enteritis virus

Liu, Fengyuan; Ma, Bo; Zhao, Yan; Zhang, Yang; Wu, Yue; Liu, Xiaomei; Wang, Junwei

doi:10.1007/s11262-008-0266-5

Cited by 24 publications

(16 citation statements)

References 17 publications

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“…Phylogenetic analysis revealed that the three deduced amino acid sequences were all closely related to mardivirus of alphaherpesvirus. The results obtained in this study are concordant with previous studies, especially with regard to the phylogenetic analysis of glycoprotein B, C, and H, which came to the same conclusion as our study [15,22,23] .…”

Section: Discussionsupporting

confidence: 93%

Characterization of Duck Enteritis Virus US6, US7 and US8 Gene

Zhao

Wang²

2010

Intervirology

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Aims: Duck enteritis virus (DEV) is a member of the family Herpesviridae, the genome of which is not completely analyzed. In order to provide further insights into the genomic organization, we decided to amplify the unknown sequence in the US region since three key glycoproteins are coded in this region. Glycoprotein D encoded by US6 gene is essential for virus entry and plays an important role in protecting animals from lethal challenge. Glycoprotein I and E encoded by US7 and US8 gene are required for cell-to-cell spread. Methods: Single oligonucleotide nested polymerase chain reaction was adopted to amplify the unknown sequence. The open reading frames (ORF) were predicted by Editseq program. Then the sequence was submitted to an online program to search promoter, polyadenylation signal and potential N-glycosylation sites. The phylogenetic analysis was performed by MEGA4.1, while multiple alignments were performed by ClustalX. Results: Three complete ORFs including US6, US7 and US8 were determined. The three genes shared the same polyadenylation signal. Phylogenetic analysis showed that DEV was closed to mardivirus of alphaherpesvirus. Conclusion: These results extend our understanding of the DEV genome and its classification, and also provide further basic knowledge for functional investigations on the proteins in the US region.

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Section: Discussionsupporting

confidence: 93%

Characterization of Duck Enteritis Virus US6, US7 and US8 Gene

Zhao

Wang²

2010

Intervirology

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“…After three frozen-thawed cycles cell lysate was then centrifuged to remove cell debris and stored at -70°C until use. Viral DNA preparation was described previously [10]. The presence of DEV was confirmed by using PCR, The primers, D1 (?)…”

Section: Methodsmentioning

confidence: 99%

Molecular analysis of US10, S3, and US2 in duck enteritis virus

et al. 2009

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A 4554-bp fragment was amplified from the DEV C-KCE vaccine strain by single oligonucleotide nested polymerase chain reaction with partially known sequences for the DEV US1 and US10 genes. Three open reading frames containing the genes encoding US10, S3, and US2 were predicted using the Editseq program (DNAStar). The S3 and US2 genes have the same transcription orientation but are oriented head-to-head with respect to US10. The promoters and polyadenylation signals were predicted. Two poly A sequences were predicted in S3, but none were predicted in US2. These results provide partial sequence of US region for the physical map of the DEV genome. Phylogenetic analysis suggests that the DEV C-KCE strain is more closely related to Mardivirus in the alphaherpesvirus subfamily of the Herpesviridae.

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“…After three frozen-thawed cycles, the cell lysate was centrifuged to remove cell debris and stored at -70°C until use. Viral DNA preparation was described previously [20]. The presence of DEV was confirmed by using PCR.…”

Section: Methodsmentioning

confidence: 99%

Molecular analysis of duck enteritis virus US3, US4, and US5 gene

Zhao

Wang

et al. 2009

Virus Genes

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Here, we first present unique short (US)3, US4, and US5 gene sequences, with analysis, of duck enteritis virus (DEV) vaccine strain C-KCE. The assembled sequence comprises 5,742 nucleotides, which are amplified from the DEV genome by single oligonucleotide-nested polymerase chain reaction with primers designed according to our previous acquired sequence deposited in GenBank (accession no. EF619046). The predicted gene arrangement is colinear with the alphaherpesvirus herpes simplex virus within the US region. The N-glycosylated sites, signal peptide, transmembrane helices, RNA polymerase II transcriptional control elements, and polyadenylation signal, were predicted with network prediction programs. Phylogenetic analysis of the three putative proteins revealed that they had a close evolutionary relationship with the subfamily of Alphaherpesvirinae.

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Characterization of the gene encoding glycoprotein C of duck enteritis virus

Cited by 24 publications

References 17 publications

Characterization of Duck Enteritis Virus <i>US6, US7</i> and <i>US8</i> Gene

Characterization of Duck Enteritis Virus <i>US6, US7</i> and <i>US8</i> Gene

Molecular analysis of US10, S3, and US2 in duck enteritis virus

Molecular analysis of duck enteritis virus US3, US4, and US5 gene

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