Characterization of the gene encoding a 60-kilodalton Babesia bovis merozoite protein with conserved and surface exposed epitopes

Suárez, Carlos E.; Palmer, Guy H.; Jasmer, Douglas P.; Hines, Stephen A.; Perryman, Lance E.; McElwain, Terry F.

doi:10.1016/0166-6851(91)90197-e

Cited by 101 publications

(71 citation statements)

References 18 publications

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“…However, the number of bovine sera was small, and further evaluation with a large number of bovine serum samples will be necessary. The CT1 and CT2 fragments contain a large region of repeated 23-aa sequences (10). The periodicity of a tandem repeated sequence is 7 from aa 317 to 477 of RAP-1, and making a secondary structure with a predicted high antigenicity is considered necessary (10).…”

Section: Discussionmentioning

confidence: 99%

Improved Enzyme-Linked Immunosorbent Assay Using C-Terminal Truncated Recombinant Antigens of Babesia bovis Rhoptry-Associated Protein-1 for Detection of Specific Antibodies

et al. 2004

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An enzyme-linked immunosorbent assay (ELISA) based on a recombinant rhoptry-associated protein-1 (RAP-1) of Babesia bovis has been previously developed, but it was imperfect because some cross-reactions were still present in Babesia bigemina-infected bovine sera. To improve its accuracy for the specific detection of the antibodies to B. bovis, we constructed three C-terminal truncated recombinant antigens of the RAP-1-rCT1 (amino acids [aa] 301 to 408), rCT2 (aa 388 to 490), and rCT3 (aa 466 to 565)-by using a baculovirus expression system and evaluated their diagnostic potentials using ELISA. rCT1 and rCT2 were better diagnostic antigens in their sensitivities and diagnostic efficiencies than rCT3, although none of the recombinant antigens showed any cross-reactivity to B. bigemina-infected bovine sera. These results confirmed that the N-terminal 300-aa region caused cross-reactivity of the entire RAP-1 antigen, and the C-terminal truncated recombinant antigens were shown to be useful reagents for species-specific serodiagnosis.

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Section: Discussionmentioning

confidence: 99%

Improved Enzyme-Linked Immunosorbent Assay Using C-Terminal Truncated Recombinant Antigens of Babesia bovis Rhoptry-Associated Protein-1 for Detection of Specific Antibodies

et al. 2004

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“…After the extraction, DNA concentration was measured using NanoDrop 2000 spectrophotometer (Thermo Scientific, NanoDrop 2000). DNA was diluted to a minimum concentration of 20 ng/μL (9)(10)(11).…”

Section: Methodsmentioning

confidence: 99%

“…Se prepararon muestras de sangre para el análisis PCR de B. bovis, B. bigemina and A. marginale al comienzo y al final del experimento (0 y 6 meses respectivamente) (9)(10)(11). Basándose en los resultados PCR, los animales que resultaron negativos para todos los agentes se agruparon separados de los considerados positivos para al menos un agente.…”

Section: Materiales Y Métodosunclassified

Influencia de la infección subclínica por agentes de la fiebre por garrapatas en vacas lecheras

et al. 2016

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Objective. The objective of this study was to evaluate the effect of subclinical infection by agents of tick fever in dairy cattle on milk parameters, such as production, composition, and quality. Materials and methods. The study was conducted in a private farm with 75 free-stall-housed dairy cows, from which 37 were evaluated. Monthly, individual milk samples were collected for compositional (fat, lactose, protein, and total solids) and quality (somatic cell counts (SCC)) analyses. In addition, blood samples were collected in order to identify cows that were tick fever-negative and positive by PCR for one or more of the following etiological agents: Babesia bovis, Babesia bigemina and Anaplasma marginale. Results. The results showed increased SCC in positive animals for at least one of the agents when compared to non-infected cows (p<0.05). Milk production was significantly lower in A. marginale positive animals (p<0.05). An increase of about 40% in milk solids content was found in B. bovis positive cows. Also, an increment of approximately 23% in lactose was found on cows positives for B. bigemina. Conclusions. We may conclude that the presence of at least one of these parasites in dairy cattle affects composition or quality of their milk.Keywords: Infectious agents, SCC, lactose, total solids (Source: CAB, MeSH). RESUMENObjetivo. El objetivo de este estudio fue evaluar el efecto de la infección subclínica por agentes de la fiebre por garrapatas en el ganado lechero en producción de leche, la composición y calidad. Materiales y métodos. El estudio se realizó en una finca privada con 75 vacas lecheras alojadas-libre puesto, y de estas se evaluaron 37. Se recogieron muestras de leche individuales mensuales para determinar la composición (grasa, lactosa, proteína y sólidos totales) y la calidad (recuento de células somáticas (SCC)). Además, se recogieron muestras de sangre para identificar vacas que fueron negativas a fiebre de garrapatas y positivos por PCR para uno o más de los siguientes agentes etiológicos: Babesia Influence of subclinical infection by agents of tick fever in milking dairy cows

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“…Isolated equine PBMC were surfacelabelled with 125I by using the lactoperoxidase-catalysed reaction (Ledbetter et al, 1981) and then were immunoprecipitated (Suarez et al, 1991). Antigen was pre-adsorbed with 1 ml of packed SepharoseProtein G beads (GammaBind Plus Sepharose, Pharmacia) for 1.5 h at room temperature to remove any labelled antigen binding to protein G alone.…”

Section: Methodsmentioning

confidence: 99%

Corticosteroid Immunosuppression and Monoclonal Antibody-mediated CD5+ T Lymphocyte Depletion in Normal and Equine Infectious Anaemia Virus-carrier Horses

Tumas¹,

Hines

Perryman³

et al. 1994

Journal of General Virology

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The immune control of chronic equine infectious anaemia (EIA) lentiviral infection was investigated by specifically depleting CD5 + T lymphocytes in vivo with monoclonal antibody (MAb) or by immunosuppression with corticosteroids. MAb was given at 25 to 50 mg/day intravenously for 11 days. Murine IgG1 anti-equine CD2 MAb (n = 2 horses) or IgG1 (n = 2) and IgG2a control MAb (n = 2 normal; 2 EIA-infected) did not deplete CD2 + T lymphocytes in horses. Horses given murine IgG2a anti-CD5 MAb HB19A (n = 4 normal; 5 EIA-infected) had depletion of peripheral blood CD5 + T lymphocytes during treatment. These horses, however, maintained a residual population of CD2 ÷ T lymphocytes [15 (_+ 3) % of pretreatment numbers] that did not express CD5 but expressed either CD4 or CD8. These antigenically modulated CDS-T lymphocytes responded normally in vivo to intradermal inoculation with phytohaemagglutinin and in vitro to allogeneic leukocyte stimulation in one-way mixed lymphocyte reactions. EIA virus-infected horses (n = 5) did not develop recrudescent viraemia or disease following in vivo CD5 + T lymphocyte depletion. Immunosuppression of EIA virus-infected horses with corticosteroids (1 mg/kg body weight/day, intravenously for 9 days) resulted in detectable recrudescent EIA viraemia in 6/11 horses (55 %) and recrudescent disease in 9/11 horses (82 %). Normal horses (n = 3) treated with corticosteroids developed no clinical disease. These results demonstrate that the use of murine IgG2a MAbs to appropriate equine lymphocyte antigens will facilitate in vivo investigation of the role of T lymphocyte subpopulations in the control of EIA or other important equine diseases.

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Characterization of the gene encoding a 60-kilodalton Babesia bovis merozoite protein with conserved and surface exposed epitopes

Cited by 101 publications

References 18 publications

Improved Enzyme-Linked Immunosorbent Assay Using C-Terminal Truncated Recombinant Antigens of Babesia bovis Rhoptry-Associated Protein-1 for Detection of Specific Antibodies

Improved Enzyme-Linked Immunosorbent Assay Using C-Terminal Truncated Recombinant Antigens of Babesia bovis Rhoptry-Associated Protein-1 for Detection of Specific Antibodies

Influencia de la infección subclínica por agentes de la fiebre por garrapatas en vacas lecheras

Corticosteroid Immunosuppression and Monoclonal Antibody-mediated CD5+ T Lymphocyte Depletion in Normal and Equine Infectious Anaemia Virus-carrier Horses

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