Characterization of the full-length genomic sequences of vesicular stomatitis Cocal and Alagoas viruses

Pauszek, Steven J.; Allende, Rossana; Rodrı́guez, Luis L.

doi:10.1007/s00705-008-0113-y

Cited by 19 publications

(16 citation statements)

References 19 publications

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“…Position -3 from the start codon of the P gene is a C, consistent with Kozak’s rules for leaky scanning through an upstream AUG codon (Kozak, 1989). The predicted DURV C protein is 136 aa in length, which was 85 aa shorter than the TUPV homolog (221 aa), but significantly larger than the C′ proteins reported for VSINV (67 aa) and VSVNJV (65 aa) or the putative C proteins reported for additional rhabdoviruses (Spiropoulou and Nichol, 1993; Schütze et al ., 1999; Marriott, 2005; Pauszek et al ., 2008; Tao et al ., 2008). BLASTp analysis of the C protein of DURV revealed that it shared homologies, covering very similar regions of the DURV C protein (encompassing aa positions 15–75) (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Like TUPV and most members of the genus Vesiculovirus , the DURV genome encodes a putative C protein from a second overlapping reading frame in the P gene (Spiropoulou and Nichol, 1993; Kretzschmar et al ., 1996; Springfeld et al ., 2005; Pauszek et al ., 2008). The DURV C protein was not conserved among related viruses, as it exhibited an 8 and 14% amino acid identity to the C proteins of TUPV and VSINV, respectively (Table 3).…”

Section: Discussionmentioning

confidence: 99%

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Characterization of Durham virus, a novel rhabdovirus that encodes both a C and SH protein

et al. 2011

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The family Rhabdoviridae is a diverse group of non-segmented, negative-sense RNA viruses that are distributed worldwide and infect a wide range of hosts including vertebrates, invertebrates, and plants. Of the 114 currently recognized vertebrate rhabdoviruses, relatively few have been well characterized at both the antigenic and genetic level; hence, the phylogenetic relationships between many of the vertebrate rhabdoviruses remain unknown. The present report describes a novel rhabdovirus isolated from the brain of a moribund American coot (Fulica americana) that exhibited neurological signs when found in Durham County, North Carolina, in 2005. Antigenic characterization of the virus revealed that it was serologically unrelated to 68 other known vertebrate rhabdoviruses. Genomic sequencing of the virus indicated that it shared the highest identity to Tupaia rhabdovirus (TUPV), and as only previously observed in TUPV, the genome encoded a putative C protein in an overlapping open reading frame (ORF) of the phosphoprotein gene and a small hydrophobic protein located in a novel ORF between the matrix and glycoprotein genes. Phylogenetic analysis of partial amino acid sequences of the nucleoprotein and polymerase proteins indicated that, in addition to TUPV, the virus was most closely related to avian and small mammal rhabdoviruses from Africa and North America. In this report, we present the morphological, pathological, antigenic, and genetic characterization of the new virus, tentatively named Durham virus (DURV), and discuss its potential evolutionary relationship to other vertebrate rhabdoviruses.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Characterization of Durham virus, a novel rhabdovirus that encodes both a C and SH protein

et al. 2011

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“…This relatively simple genome organization is similar to that of vesiculoviruses. 30 Based on the N and L phylogenies and full genome nt sequence pairwise comparisons, the ledanteviruses can be further subdivided into three subgroups. This is partially supported by the complement fixation tests reported previously, although strong serological cross-reactions were generally only observed between closely related viruses (BARV and FUKV; KEUV, and LEDV); NISV and FKRV were not included in this study.…”

Section: Discussionmentioning

confidence: 99%

Ledantevirus: A Proposed New Genus in the Rhabdoviridae has a Strong Ecological Association with Bats

Blasdell¹,

Guzmán²,

Widen³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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The Le Dantec serogroup of rhabdoviruses comprises Le Dantec virus from a human with encephalitis and Keuriliba virus from rodents, each isolated in Senegal. The Kern Canyon serogroup comprises a loosely connected set of rhabdoviruses many of which have been isolated from bats, including Kern Canyon virus from California, Nkolbisson virus from Cameroon, Central African Republic, and Cote d'Ivoire, Kolente virus from Guinea, Mount Elgon bat and Fikirini viruses from Kenya, and Oita virus from Japan. Fukuoka virus isolated from mosquitoes, midges, and cattle in Japan, Barur virus from a rodent in India and Nishimuro virus from pigs in Japan have also been linked genetically or serologically to this group. Here, we analyze the genome sequences and phylogenetic relationships of this set of viruses. We show that they form three subgroups within a monophyletic group, which we propose should constitute the new genus Ledantevirus.

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“…13 The product of the current research is a field-evaluated, rapid, robust, realtime RT-PCR that identifies all VS virus isolates in Central and North America in as little as 4 hr using dry reagents and portable instrumentation. The assay is selfcorrecting in that the isolation of VS virus from a PCRnegative case signals a previously unrecognized virus that can be sequenced to provide a rapid modification of the assay.…”

Section: Discussionmentioning

confidence: 99%

Field Evaluation of a Multiplex Real-Time Reverse Transcription Polymerase Chain Reaction Assay for Detection of Vesicular Stomatitis Virus

et al. 2009

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Abstract. Sporadic outbreaks of vesicular stomatitis (VS) in the United States result in significant economic losses for the U.S. livestock industries because VS is a reportable disease that clinically mimics footand-mouth disease. Rapid and accurate differentiation of these 2 diseases is critical because their consequences and control strategies differ radically. The objective of the current study was to field validate a 1-tube multiplexed real-time reverse transcription polymerase chain reaction (real-time RT-PCR) assay for the rapid detection of Vesicular stomatitis New Jersey virus and Vesicular stomatitis Indiana virus strains occurring in Mexico and North and Central America. A comprehensive collection of 622 vesicular lesion samples obtained from cattle, horses, and swine from throughout Mexico and Central America was tested by the real-time RT-PCR assay and virus isolation. Overall, clinical sensitivity and specificity of the real-time RT-PCR were 83% and 99%, respectively. Interestingly, VS virus isolates originating from a specific region of Costa Rica were not detected by real-time RT-PCR. Sequence comparisons of these viruses with the real-time RT-PCR probe and primers showed mismatches in the probe and forward and reverse primer regions. Additional lineage-specific primers and a probe corrected the lack of detection of the missing genetic lineage. Thus, this assay reliably identified existing Mexican and Central American VS viruses and proved readily adaptable as new VS viruses were encountered. An important secondary result of this research was the collection of hundreds of new VS virus isolates that provide a foundation from which many additional studies can arise.

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Characterization of the full-length genomic sequences of vesicular stomatitis Cocal and Alagoas viruses

Cited by 19 publications

References 19 publications

Characterization of Durham virus, a novel rhabdovirus that encodes both a C and SH protein

Characterization of Durham virus, a novel rhabdovirus that encodes both a C and SH protein

Ledantevirus: A Proposed New Genus in the Rhabdoviridae has a Strong Ecological Association with Bats

Field Evaluation of a Multiplex Real-Time Reverse Transcription Polymerase Chain Reaction Assay for Detection of Vesicular Stomatitis Virus

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