Characterization of the extra-intestinal pathogenic Escherichia coli ST131 clone among isolates recovered from urinary and bloodstream infections in the United Kingdom

Abstract

The multidrug-resistant ST131-O25b clone of Escherichia coli is well established as a significant cause of extra-intestinal infections worldwide. However, there have been only two small regional studies comparing ST131 isolates from the UK. Therefore, we characterized 143 ST131 E. coli (38 urinary, 105 bloodstream) collected between January 2011 and March 2012 from 38 centres located across the UK and Republic of Ireland. Phenotypic and genotypic characterization of clonal isolates revealed high rates of resistance to amoxicillin-clavulanate (56 %), cefotaxime (32 %), ciprofloxacin (79 %), temocillin (69 %, bloodstream isolates only), gentamicin (67 %) and trimethoprim-sulfamethoxazole (59 %). The most frequently detected extended-spectrum beta-lactamase was CTX-M-15 (87 %), predominantly encoded on IncF plasmids, although it was also associated with IncU plasmids in two isolates. The majority of UK ST131 clonal isolates possessed the O25b antigen (97 %) and the H30 fimH allele (92 %), but three serogroups (O19a, O136 and O153) novel to ST131 were identified among our strains. Contrary to previous reports, UK ST131-O16 isolates were typically susceptible to ciprofloxacin and lacked beta-lactamase genes (n512/12). In summary, ST131 strains of E. coli circulating in the UK possess characteristic clonal features, but are becoming more diverse than other international ST131 populations.

Comprehensive Molecular Characterization of Escherichia coli Isolates from Urine Samples of Hospitalized Patients in Rio de Janeiro, Brazil

“…In addition, the O25:H4/ST131 CTX-M-producing isolates in this study were also found to be resistant to gentamicin, tobramycin, and ciprofloxacin. This is similar to data presented in studies worldwide that showed that CTX-M-producing isolates are often MDR (Pitout and Laupland, 2008 ; Ewers et al, 2014 ; Ciesielczuk et al, 2015 ).…”

Emergence of Escherichia coli ST131 Causing Urinary Tract Infection in Western Asia: A Systematic Review and Meta-Analysis

“…In this study, 24.6% (95% CI: 13.5%-40.4%) was the pooled prevalence of ST131 clone among WT isolates over the past decade in western Asia. The prevalence reported in our review was closest to those reported among patients with UTI in the United States (29.4%), 35 Brazil (24.07%), 36 and Canada (23.1%) 37 ; however, it was lower than those reported from China (15.4%), 18 Korea (13.2%), 38 the United Kingdom (7%), 39 and the Netherlands (4%). 40 Our findings suggest that ST131 clone occurrence among ESBL-producing E. coli is 42.7% (95% CI: 32.5%-53.5%).…”

Genomic Characterization of Escherichia coli Isolates Belonging to a New Hybrid aEPEC/ExPEC Pathotype O153:H10-A-ST10 eae-beta1 Occurred in Meat, Poultry, Wildlife and Human Diarrheagenic Samples

“…According to the authors, the presence of virulent and MDR aEPEC in retail foods poses a potential threat to consumers. While O153 is a serogroup reported within ExPEC and DEC isolates, and linked to different lineages [33][34][35][36], there are few references of the serotype O153:H10. Notheworthy, the report by Schremmer et al [37] of an aEPEC O153:H10 isolated from the small intestine of a cockatiel (psittaciform bird) with enteritis.…”