Characterization of the European Sea Bass (Dicentrarchus labrax) Gonadal Transcriptome During Sexual Development

Ribas, Laia; Crespo, Berta; Sánchez-Baizán, Núria; Xavier, Daniela; Kuhl, Heiner; Rodriguez, José Manuel; Díaz, Noèlia; Boltaña, Sebastián; Mackenzie, Simon; Morán, Federico; Zanuy, Silvia; Gómez, Ana; Piferrer, Francesc

doi:10.1007/s10126-019-09886-x

Cited by 31 publications

(27 citation statements)

References 97 publications

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“…Subsequently, knockdown of KDM6B in T. scripta caused male to female sex reversal by removing methylation marks on the promoter of DMRT1 , a gene critical in the male sex determination pathway (15). KDM6B and JARID2 have also been associated with TSD in another turtle species ( Chrysemys picta ) (126), female to male sex change in the bluehead wrasse, Thalassoma bifasciatum (140), and thermal responses in the European bass, Dicentrarchus labrax (141).…”

Section: Discussionmentioning

confidence: 99%

Two transcriptionally distinct pathways drive female development in a reptile with both genetic and temperature dependent sex determination

Whiteley

Holleley

Wagner

et al. 2021

Preprint

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How temperature determines sex remains unknown. A recent hypothesis proposes that conserved cellular mechanisms (calcium and redox; CaRe status) sense temperature and identify genes and regulatory pathways likely to be involved in driving sexual development. We take advantage of the unique sex determining system of the model organism, Pogona vitticeps, to assess predictions of this hypothesis. P. vitticeps has ZZ male: ZW female sex chromosomes whose influence can be overridden in genetic males by high temperatures, causing male-to-female sex reversal. We compare a developmental transcriptome series of ZWf females and temperature sex reversed ZZf females. We demonstrate that early developmental cascades differ dramatically between genetically driven and thermally driven females, later converging to produce a common outcome (ovaries). We show that genes proposed as regulators of thermosensitive sex determination play a role in temperature sex reversal. Our study greatly advances the search for the mechanisms by which temperature determines sex.

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Section: Discussionmentioning

confidence: 99%

Two transcriptionally distinct pathways drive female development in a reptile with both genetic and temperature dependent sex determination

Whiteley

Holleley

Wagner

et al. 2021

Preprint

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“…We also assessed the effect of genetic origin of parents (wild or selected for growth) on sex ratio. In order to mimic commercial sea bass hatchery conditions, we increased temperature from 16 to 19 °C between 16 and 21 days post hatching (dph), long before the supposed window of molecular sex determination (between 110 and 150 dph) 31 , 32 . We monitored cortisol release in the water during this period for all experimental conditions to evaluate to what extent temperature correlated with this stress hormone.…”

Section: Introductionmentioning

confidence: 99%

Parental selection for growth and early-life low stocking density increase the female-to-male ratio in European sea bass

Geffroy

Gesto

Clota

et al. 2021

Sci Rep

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In European sea bass (Dicentrarchus labrax), as in many other fish species, temperature is known to influence the sex of individuals, with more males produced at relatively high temperatures. It is however unclear to what extent growth or stress are involved in such a process, since temperature is known to influence both growth rate and cortisol production. Here, we designed an experiment aiming at reducing stress and affecting early growth rate. We exposed larvae and juveniles originating from both captive and wild parents to three different treatments: low stocking density, food supplemented with tryptophan and a control. Low stocking density and tryptophan treatment respectively increased and decreased early growth rate. Each treatment influenced the stress response depending on the developmental stage, although no clear pattern regarding the whole-body cortisol concentration was found. During sex differentiation, fish in the low-density treatment exhibited lower expression of gr1, gr2, mr, and crf in the hypothalamus when compared to the control group. Fish fed tryptophan displayed lower crf in the hypothalamus and higher level of serotonin in the telencephalon compared to controls. Overall, fish kept at low density produced significantly more females than both control and fish fed tryptophan. Parents that have been selected for growth for three generations also produced significantly more females than parents of wild origin. Our findings did not allow to detect a clear effect of stress at the group level and rather point out a key role of early sexually dimorphic growth rate in sex determination.

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“…Also, it provides the first steps toward functional characterization and annotation of genes, and it produces molecular fingerprints of disease processes (Morozova et al, 2009). For example, a succession of studies using the second-generation RNA-Seq to evaluate the potential mechanism and probable markers in aquatic animal diseases has been performed in recent years (Morozova et al, 2009;Stockhammer et al, 2010;Ordas et al, 2011;Zhang et al, 2011;Ribas et al, 2019). The role of innate immune response to bacterial evasion in zebrafish embryos was determined by transcriptome analysis, which also indicated a series of differentially expressed genes (DEGs) linked to the immune response activities (Stockhammer et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Identification of Multigene Biomarker for Shrimp White Feces Syndrome by Full-Length Transcriptome Sequencing

et al. 2020

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The pacific white shrimp, Litopenaeus vannamei, with the largest shrimp industry production in the world, is currently threatened by a severe disease, white feces syndrome (WFS), which cause devastating losses globally, while its causal agents remain largely unknown. Herein, compared to the Control shrimp by metagenomic analysis, we firstly investigated that the altered functions of intestinal microbial community in WFS shrimp were the enrichment of bacterial chemotaxis and flagellar assembly pathways, hinting at a potential role of pathogenic bacteria for growth and development, which might be related to WFS occurrence. Single-molecule real-time (SMRT) sequencing was to further identify the gene structure and gene regulation for more clues in WFS aetiology. Totally 50,049 high quality transcripts were obtained, capturing 39,995 previously mapped and 10,054 newly detected transcripts, which were annotated to 30,554 genes. A total of 158 differentially expressed genes (DEGs) were characterized in WFS shrimp. These DEGs were strongly associated with various immune related genes that regulated the expression of multiple antimicrobial peptides (e.g., antilipopolysaccharide factors, penaeidins, and crustin), which were further experimentally validated using quantitative PCR on transcript level. Collectively, multigene biomarkers were identified to be closely associated with WFS, especially those functional alterations in microbial community and the upregulated immune related gene with antibacterial activities. Our finding not only inspired our cogitation on WFS aetiology from both microbial and host immune response perspectives with combined metagenomic and full-length transcriptome sequencing, but also provided valuable information for enhancing shrimp aquaculture.

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Characterization of the European Sea Bass (Dicentrarchus labrax) Gonadal Transcriptome During Sexual Development

Cited by 31 publications

References 97 publications

Two transcriptionally distinct pathways drive female development in a reptile with both genetic and temperature dependent sex determination

Two transcriptionally distinct pathways drive female development in a reptile with both genetic and temperature dependent sex determination

Parental selection for growth and early-life low stocking density increase the female-to-male ratio in European sea bass

Identification of Multigene Biomarker for Shrimp White Feces Syndrome by Full-Length Transcriptome Sequencing

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