Characterization of the Ets-type protein ER81 in Xenopus embryos

Chen, Yonglong; Hollemann, Thomas; Grunz, Horst; Pieler, Tomas

doi:10.1016/s0925-4773(98)00194-4

Cited by 26 publications

(38 citation statements)

References 42 publications

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“…Anterior truncations and posterior duplications similar to those reported here have been observed with the misexpression of several FGF signaling pathway components, including eFGF, Xbra, Laloo, Gli2, XER81, and SNT1 (Pownall et al, 1996;Tada et al, 1997;Weinstein et al, 1998;Chen et al, 1999;Brewster et al, 2000;Hama et al, 2001). In Xenopus embryos, it has also recently been shown that overexpression of the EphA4 receptor induced ectopic structures, but the mechanism was dependent upon activation of the FGF signaling pathway as well (Park et al, 2004).…”

Section: Discussionsupporting

confidence: 82%

Oncogenic Met receptor induces ectopic structures in Xenopus embryos

Ishimura

et al. 2006

Oncogene

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When aberrantly expressed or activated, the Met receptor tyrosine kinase is involved in tumor invasiveness and metastasis. In this study, we have used the Xenopus embryonic system to define the role of various Met proximal-binding partners and downstream signaling pathways in regulating an induced morphogenetic event. We show that expression of an oncogenic derivative of the Met receptor (Tpr-Met) induces ectopic morphogenetic structures during Xenopus embryogenesis. Using variant forms of Tpr-Met that are engineered to recruit a specific signaling molecule of choice, we demonstrate that the sole recruitment of either the Grb2 or the Shc adaptor protein is sufficient to induce ectopic structures and anterior reduction, while the recruitment of PI-3Kinase (PI-3K) is necessary but not sufficient for this effect. In contrast, the recruitment of PLCc can initiate the induction, but fails to maintain or elongate supernumerary structures. Finally, evidence indicates that the Ras/Raf/MAPK pathway is necessary, but not sufficient to induce these structures. This study also emphasizes the importance of examining signaling molecules in the regulatory context that is provided by receptor/effector interactions when assessing a role in cell growth and differentiation.

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Section: Discussionsupporting

confidence: 82%

Oncogenic Met receptor induces ectopic structures in Xenopus embryos

Ishimura

et al. 2006

Oncogene

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“…Nevertheless a high proportion of injected embryos (>50%) still showed strong gastrulation defects. A relatively high proportion of the embryos showed tail-like protrusions, as was reported for XER81 overexpression experiments (Chen et al, 1999). However, in those few injected embryos that showed no gastrulation defects and could be used for a further analysis, a strong induction of the expression of endothelial marker genes AMI and msr could be observed at the sites of injection (Fig.…”

Section: Overexpression Of Er71 Leads To Ectopic Endothelial Gene Expsupporting

confidence: 72%

Xenopus er71 is involved in vascular development

Neuhaus

Müller

Hollemann

2010

Developmental Dynamics

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Vasculogenesis and hematopoiesis are closely linked in developing vertebrates. Recently, the existence of a common progenitor of these two tissues, the hemangioblast, has been demonstrated in different organisms. In Xenopus early vascular and hematopoietic cells differentiate in a region called the anterior ventral blood island (aVBI). Differentiating cells from this region migrate out to form embryonic blood and part of the vascular structures of the early frog embryo. A number of members of the ETS family of transcription factors are expressed in endothelial cells and some of them play important roles at various stages of vascular development. The loss of ER71 function in mice led to a complete loss of blood and vascular structures. Similarly, knock down of the zebrafish homolog of er71, etsrp, greatly affected development of vascular structures and myeloid cells. We have identified the Xenopus ortholog of er71 and could show that er71 function in Xenopus is required for vasculogenesis, but not for the development of hematopoietic cells. Developmental Dynamics 239:3436-3445,

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“…Future studies can thus be performed to assess the importance of this Pax6 site for the pallial activity of the zER812-and 1.5-kb fragments. In addition, the expression of mouse (Hasegawa et al, 2004) and Xenopus (Chen et al, 1999;Munchberg and Steinbeisser, 1999) ER81 orthologues are regulated by fibroblast growth factor (FGF) signaling, whereas zebrafish ER81 expression appears to be FGFindependent (Roussigne and Blader, 2006). This suggests that the regulatory interactions governing ER81 expression were altered after terrestrial vertebrates branched off from fish 365 million years ago (Carroll, 1999).…”

Section: Pallial Regulatory Elements From Zebrafish Er81 Are Active Imentioning

confidence: 99%

Validating in utero electroporation for the rapid analysis of gene regulatory elements in the murine telencephalon

Langevin

Mattar

Scardigli

et al. 2007

Developmental Dynamics

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With the ultimate goal of understanding how genetic modules have evolved in the telencephalon, we set out to modernize the functional analysis of cross-species cis-regulatory elements in mouse. In utero electroporation is rapidly replacing transgenesis as the method of choice for gain-and loss-of-function studies in the murine telencephalon, but the application of this technique to the analysis of transcriptional regulation has yet to be fully explored and exploited. To empirically define the developmental stages required to target specific populations of neurons in the dorsal telencephalon, or pallium, which gives rise to the neocortex in mouse, we performed a temporal and spatial analysis of the migratory properties of electroporated versus birth-dated cells. Next, we compared the activities of two known Ngn2 enhancers via transgenesis and in utero electroporation, demonstrating that the latter technique more faithfully reports the endogenous telencephalic expression pattern observed in an Ngn2lacZ knock-in line. Finally, we used this approach to test the telencephalic activities of a series of deletion constructs comprised of the zebrafish ER81 upstream regulatory region, allowing us to identify a previously uncharacterized enhancer that displays cross-species activity in the murine piriform cortex and lateral neocortex, yet not in more medial domains of the forebrain. Taken together, our data supports the contention that in utero technology can be exploited to rapidly examine the architecture and evolution of pallial-specific cis-regulatory elements.

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Characterization of the Ets-type protein ER81 in Xenopus embryos

Cited by 26 publications

References 42 publications

Oncogenic Met receptor induces ectopic structures in Xenopus embryos

Oncogenic Met receptor induces ectopic structures in Xenopus embryos

Xenopus er71 is involved in vascular development

Validating in utero electroporation for the rapid analysis of gene regulatory elements in the murine telencephalon

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