Characterization of the enzymatic tail protein of bacteriophage P22

Eriksson, Urban; Lindberg, Annelie

doi:10.1016/0014-5793(76)80803-4

Cited by 7 publications

(5 citation statements)

References 10 publications

(12 reference statements)

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“…Mapping of the tryptic peptides of [~4C]carboxymethylated chicken-liver alcohol dehydrogenase, by electrophoresis and paper chromatography [ 16] followed by ninhydrin staining, revealed 42 major spots, which is close to the number expected from the total amount of lysine and arginine (table 2). Autoradiography showed 10 peptides to be strongly radioactive (5 neutral, 5 acidic), while 2 others (also acidic) were less radioactive and may be minor fragments due to peptide bonds only partially sensitive to tryptic digestion.…”

Section: Structural Characteristicsmentioning

confidence: 61%

Purification and characterization of chicken liver alcohol dehydrogenase

et al. 1978

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Section: Structural Characteristicsmentioning

confidence: 61%

Purification and characterization of chicken liver alcohol dehydrogenase

et al. 1978

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“…M~ikel~i, Central Public Health Laboratory, Helsinki, Finland. 1) [6]. Partial hydrolysis of the lipid moiety and release of 2-O-acetyl groups from abequose (O-antigen 5 determinant) was achieved by boiling the LPS for 1 h in 0.15 M NaOH.…”

Section: Methodsmentioning

confidence: 99%

“…The bacteria were grown and LPS extracted as described earlier [8]. After centrifugation (60 000 × g for 1 h) to sediment phage the supernatant was collected, and subsequently purified by gel chromatography [6]. Bacteriophage P22c2 was propagated and purified as described previously [6].…”

Section: Methodsmentioning

confidence: 99%

“…A sensitive in vivo assay demonstrated that, in immunized mice, after challenge with two strains of equal virulence but with different O-antigens, the growth of the strain with an O-antigen homologous to that of the immunogen was suppressed in comparison to that of the control strain [4]. The enzyme was the tail protein of phage P22 [6]. We herein describe a novel approach for the production of artificial oligosaccharide containing immunogens.…”

Section: Introductionmentioning

confidence: 99%

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Oligosaccharide-protein conjugate: A novel approach for making Salmonella O-antigen immunogens

Svenson¹

1977

FEMS Microbiology Letters

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“…A schematic representation of bacteriophage P22 is shown in Figure 5. To find its host bacterium, Salmonella typhimurium, the tailspikes of bacteriophage P22 seek and recognize lipopolysaccharide receptors on the outer membrane of the bacterium (Berget and Poteete, 1980;Eriksson and Lindberg, 1977;Eriksson et al,, 1976Eriksson et al,, , 1979Israel et al, 1972;Kanegasaki, 1973, 1976). Although the tailspikes are known to have a biospecific affinity toward the polysaccharide portion of the receptors, they still may exhibit attractive interactions with the lipid portion of the lipopolysaccharide.…”

Section: Introductionmentioning

confidence: 99%

Understanding viral partitioning in two‐phase aqueous nonionic micellar systems: 1. Role of attractive interactions between viruses and micelles

Kamei

Liu

Haase-Pettingell

et al. 2002

Biotech & Bioengineering

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The partitioning behavior of viruses in the two-phase aqueous nonionic n-decyl tetra(ethylene oxide) (C10E4) micellar system cannot be fully explained by considering solely the repulsive, steric, excluded-volume interactions that operate between the viruses and the nonionic C10E4 micelles. Specifically, an excluded-volume theory developed recently by our group is not able to quantitatively predict the observed viral partition coefficients, even though this theory is capable of providing reasonable quantitative predictions of protein partition coefficients. To shed light on the discrepancy between the theoretically predicted and the experimentally measured viral partition coefficients, a central assumption underlying the excluded-volume theory that the viruses and the C10E4 micelles interact solely through repulsive, excluded-volume interactions was challenged in this study. In particular, utilizing bacteriophage P22 as a model virus, a competitive inhibition test and a partitioning study of the capsids of bacteriophage P22 were conducted. Based on the results of these two experimental studies, it was concluded that any attractive interactions between the tailspikes of bacteriophage P22 and the C10E4 micelles are negligible. Another experimental study was carried out wherein the partition coefficients of the model viruses, bacteriophages P22 and T4, were measured at various temperatures, and compared with those previously obtained for bacteriophage phiX174. This comparison also indicated that possible attractive, electromagnetic-induced interactions between the bacteriophage particles and the C10E4 micelles cannot be invoked to rationalize the observed discrepancy between the theoretically predicted and the experimentally measured viral partition coefficients.

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Characterization of the enzymatic tail protein of bacteriophage P22

Cited by 7 publications

References 10 publications

Purification and characterization of chicken liver alcohol dehydrogenase

Purification and characterization of chicken liver alcohol dehydrogenase

Oligosaccharide-protein conjugate: A novel approach for making Salmonella O-antigen immunogens

Understanding viral partitioning in two‐phase aqueous nonionic micellar systems: 1. Role of attractive interactions between viruses and micelles

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