Characterization of the enterovirus 71 P1 polyprotein expressed in<i>Pichia pastor</i>as a candidate vaccine

Han, Xue; Ying, Xiaoling; Zhou, Suna; Han, Tao; Huang, Hao; Qi, Jin; Yang, Fan; Sun, Qiangming; Sun, Xiao

doi:10.4161/hv.29123

Cited by 7 publications

(3 citation statements)

References 52 publications

(53 reference statements)

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“…36 Such high mutation rate allows EV-A71 to adapt rapidly to selection pressures, which select for beneficial mutations. 37 It can be inferred that the epitope of the C4 strain functional protein has changed in 16 years, 38 , 39 and the epitope on the surface protein may make the immune response anti-infective. The type has a certain degree of cross-neutralization.…”

Section: Discussionmentioning

confidence: 99%

Effect of difference between EV-A71 virus epidemic strain and “vaccine strain” on neutralizing antibody titer

Ding¹,

Zhao²

2022

Human Vaccines & Immunotherapeutics

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Hand, foot and mouth disease was mainly caused by EV-A71 virus. The main antigen structure of VP1 region of EV-A71 was easily varied. Here, we investigated the seroprevalence of EV-A71 based on a large group of healthy individuals in Beijing, China, in order to study the effectiveness of EV-A71 vaccine in a real-world setting. BrCr and the clinical strain isolated from the Chinese mainland in 2008 (“vaccine strain:”CMU4232/BJ/CHN/2008), EV-A71 C4 epidemic strains isolated in 2010, 2013, and 2016, were tested for neutralizing antibodies (NtAb) in every year. Phylogenetic tree analysis of the EV-A71 strains above, as well as amino acid composition homologous sequence analysis were applied. The “vaccine strain” has 83.0% homology with FY23, H07 and FY7VP5. It belongs to the same branch of C4a as 10 C4, 13 C4 and 16 C4, and differs from the amino acid sites 283 and 293 of 16 C4. Compared with “vaccine strains,” there was a significant difference between the 50–59 years old age group when the NtAb titer of 16 C4 strain was 1:512-1:1024. Our results suggest that changes in the functional epitopes of NtAb caused by amino acid 283 and 293 loci in EV-A71 strains may affect the production of neutralizing antibodies.

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Section: Discussionmentioning

confidence: 99%

Effect of difference between EV-A71 virus epidemic strain and “vaccine strain” on neutralizing antibody titer

Ding¹,

Zhao²

2022

Human Vaccines & Immunotherapeutics

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“…EV71 is the leading causative agent for HFMD. Until now, a number of approaches have been taken to develop vaccines against EV71, including inactivated whole-virus vaccines [7][8][9][10], attenuated live virus vaccines [11], viruslike particles (VLPs) [12][13][14][15][16][17] and other types of EV71 vaccine candidates [18][19][20][21][22][23][24][25][26][27] (Table 1).…”

Section: Vaccine Development Against Ev71mentioning

confidence: 99%

“…There are also other types of EV71 vaccine candidates. The precursor of EV71 capsid proteins P1, expressed in P. pastoris, was shown to be immunogenic and capable of eliciting improved humoral and cellular immune responses while also providing good cross-protection with different EV71 strains compared with the heat-inactivated EV71 [18].…”

Section: Ev71 P1 or Vp1 As The Immunogenmentioning

confidence: 99%

Achievements, challenges and prospects for the development of broadly protective multivalent vaccines and therapeutic antibodies against hand, foot and mouth disease

Lyu

Chen

2015

Science Bulletin

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Efficient production of recombinant glycoprotein D of herpes simplex virus type 2 in Pichia pastoris and its protective efficacy against viral challenge in mice

et al. 2016

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Herpes simplex virus type 2 (HSV-2) infection is the leading cause of genital ulcer disease and a significant public health concern. However, there are no approved vaccines available to prevent HSV-2 infection. The glycoprotein D (gD) of HSV-2 is the most important candidate antigen for vaccine development. In this study, a truncated form of gD (codons 1-340, gD) was produced as a secretory protein in the methylotrophic yeast Pichia pastoris. The recombinant gD with a His tag was purified to homogeneity by one-step affinity chromatography. Mice immunized with the recombinant gD developed high levels of antigen-specific antibody responses with HSV-2 neutralizing activity. Immunization with the recombinant gD conferred significant protection against lethal HSV-2 infection in mice. Moreover, measurement of the secretion of gD-specific cytokines demonstrated that the recombinant gD induced mixed Th1/Th2 cellular immune responses. These findings indicated that P. pastoris-derived gD represents a promising HSV-2 vaccine candidate with strong immunogenicity and prophylactic efficacy.

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Characterization of the enterovirus 71 P1 polyprotein expressed inPichia pastoras a candidate vaccine

Cited by 7 publications

References 52 publications

Effect of difference between EV-A71 virus epidemic strain and “vaccine strain” on neutralizing antibody titer

Effect of difference between EV-A71 virus epidemic strain and “vaccine strain” on neutralizing antibody titer

Achievements, challenges and prospects for the development of broadly protective multivalent vaccines and therapeutic antibodies against hand, foot and mouth disease

Efficient production of recombinant glycoprotein D of herpes simplex virus type 2 in Pichia pastoris and its protective efficacy against viral challenge in mice

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