Characterization of the endogenous enzymatic hydrolyses of Petroselinum crispum glycosides: Determined by chromatography upon their sugar and flavonoid products

Determination of Impurities in Bioproduced Succinic Acid

“…Most of these studies characterize food products, focusing on relevant species occurring in fairly high concentration [4][5][6][7][8][9][16][17][18]. To our knowledge, no shortchain (i.e., highly volatile) monocarboxylic acids were reported.…”

“…The most common approach is derivatization with hydroxylamine in pyridine in combination with hexamethyldisilazane (HMDS) with trifluoroacetic acid [5][6][8][9][10], where hydroxylamine reacts with the saccharide carbonyl group while HMDS functionalizes the moiety containing a reactive hydrogen atom, i.e., carboxyl, hydroxyl and phenyl groups. However, the use of two derivatization agents may lead to uncertainties as the optimal conditions for two different derivatizations may not match.…”

“…Numerous studies addressing acids, saccharides and polyalcohols were performed using GC-MS with trimethylsilylation [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] (Supplemental Table S.1 for their overview). Most of these studies characterize food products, focusing on relevant species occurring in fairly high concentration [4][5][6][7][8][9][16][17][18].…”

Quantitative determination of isoflavonoids in Ononis species by UPLC‐UV‐DAD

“…A further approach for the hydrolysis of the glucosides is to use β-glucosidase enzymes, 30 either from an external source or based on the plant's endogenous β-glucosidase. 31 Treating the samples with water to activate the endogenous glucosidase enzymes could free the aglycones from their glycosidic storage, but this reaction was not quantitative. As can be seen in Figure 2(C), the malonates were erased from the sample and the level of the aglycones rose, while the amount of the glucosides dropped, but still a reasonable quantity of glucosides remained in the samples.…”

A Medium for Facilitating Hepatitis B Virus Detection and Replication of the Virus

“…interface temperature as 280ºC, and solvent cut time as 2.55 minutes. These parameters were measured as in the literature [7][8].…”