Characterization of the drug resistance plasmid NTP16

Lambert, Clare M.; Wrighton, Christopher J.; Strike, Peter

doi:10.1016/0147-619x(87)90005-9

Cited by 9 publications

(2 citation statements)

References 26 publications

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“…ColEl is a 6.6-kb E. coli plasmid with a copy number of nearly 20 (6,38). Related multicopy plasmids of members of the family Enterobacteriaceae include plSA, pMB1, RSF1010 (NTP1), CloDF13 (259), NTP16 (158), and other coligenic plasmids (116,196,370,371). Several multicopy cloning vectors such as the well-known plasmids pACYC184 and pBR322 are derived from plSA and pMB1 (13,39).…”

Section: Colel and Related Plasmidsmentioning

confidence: 99%

Replication of plasmids in gram-negative bacteria.

Kües¹,

Ståhl²

1989

Microbiological Reviews

134

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Section: Colel and Related Plasmidsmentioning

confidence: 99%

Replication of plasmids in gram-negative bacteria.

Kües¹,

Ståhl²

1989

Microbiological Reviews

134

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“…A similar explanation has been suggested for the presence of two genes in another ColE1-type plasmid from Salmonella spp. Plasmid NTP16 is a small, multicopy nonconjugative plasmid from S. enterica serovar Typhimurium and is related to Salmonella plasmid NTP1 (24). NTP16 carries transposon-derived sequences including genes for ampicillin and kanamycin resistance.…”

Section: Discussionmentioning

confidence: 99%

Lateral transfer of rfb genes: a mobilizable ColE1-type plasmid carries the rfbO:54 (O:54 antigen biosynthesis) gene cluster from Salmonella enterica serovar Borreze

Keenleyside

Whitfield

1995

J Bacteriol

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Plasmid pWQ799 is a 6.9-kb plasmid isolated from Salmonella enterica serovar Borreze. Our previous studies have shown that the plasmid contains a functional biosynthetic gene cluster for the expression of the O:54 lipopolysaccharide O-antigen of this serovar. The minimal replicon functions of pWQ799 have been defined, and a comparison with nucleotide and protein databases revealed this replicon to be virtually identical to ColE1. This is the first report of involvement of ColE1-related plasmids in O-antigen expression. The replicon of pWQ799 is predicted to encode two RNA molecules, typical of other ColE1-type plasmids. RNAII, the putative replication primer from pWQ799, shares regions of homology with RNAII from ColE1. RNAI is an antisense regulator of DNA replication in ColE1-related plasmids. The coding region for RNAI from pWQ799 shares no homology with any other known RNAI sequence but is predicted to adopt a secondary structure characteristic of RNAI molecules. pWQ799 may therefore represent a new incompatibility group within this family. pWQ799 also possesses cer, rom, and mob determinants, and these differ minimally from those of ColE1. The plasmid is mobilizable in the presence of either the broad-host-range helper plasmid pRK2013 or the IncI 1 plasmid R64drd86. Mobilization and transfer of pWQ799 to other organisms provides the first defined mechanism for lateral transfer of O-antigen biosynthesis genes in S. enterica and explains both the distribution of related plasmids and coexpression of the O:54 factor with other O-factors in different Salmonella serovars. The base composition of the pWQ799 replicon sequences gives an average percent G؉C value typical of Salmonella spp. In contrast, the percent G؉C value is dramatically lower within rfb O:54 , consistent with the possibility that the cluster was acquired from an organism with a much lower G؉C composition.

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