CHARACTERIZATION OF THE DIFFERENT FORMS OF PHOSPHORUS IN THE MYCELIUM OF THE ECTOMYCORRHIZAL FUNGUS, <i>HEBELOMA CYLINDROSPORUM</i>, CULTIVATED IN PURE CULTURE

Tacon, François Le; Larher, F.

doi:10.1111/j.1469-8137.1984.tb02744.x

Cited by 22 publications

(19 citation statements)

References 14 publications

(15 reference statements)

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“…They concluded that these granules were the sites of P accumulation observed with radioactive tracer experiments. Accumulations of polyP have been observed also in mycorrhizal fungi grown in culture (17,18,26). Later work has shown that uptake of 32P by mycorrhizae is first into a Pi pool, followed by accumulation as polyP (7).…”

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confidence: 78%

Phosphorus Distribution in Red Pine Roots and the Ectomycorrhizal Fungus Hebeloma arenosa

MacFall¹,

Slack²,

Wehrli³

1992

Plant Physiol.

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Red pines (Pinus resinosa Ait.) were grown in a pasteurized sandy loam either unamended with phosphate or fertilized with one of two levels of phosphate (34 or 136 mg/kg) as superphosphate, and with and without addition of Hebeloma arenosa inoculum. Shoot and total dry weights of mycorrhizal seedlings grown in soil unamended with P were greater than those for nonmycorrhizal seedlings grown in the same soil, but less than the dry weights of seedlings grown in soil amended with middle to high levels of P. Mycorrhizal infection was inhibited at the highest level of P amendment. 31P nuclear magnetic resonance spectra of intact mycorrhizal roots showed the presence of two dominant peaks, orthophosphate (Pi) and polyphosphate (polyP). The polyP peak was absent in spectra of nonmycorrhizal roots. The ratio for areas under the two peaks, Pi/polyP, was 1.8 for mycorrhizal roots grown in both unamended soil and soil that had received middle levels of superphosphate. Apparently, the fungus strongly mediates the supply of phosphate to the tree through the production of polyP, even at growth-limiting levels of soil P, and regulates compartmentalization of P in the mycorrhizal roots.Mycorrhizal fungi can take up, store, and translocate P while forming mycorrhizal associations with the host plant (6, 9). Autoradiographic studies have shown uptake and translocation of P both toward the host plant and toward the hyphal tips through the fungal mycelium (4, 11), as well as accumulations within the mycorrhizae.Harley and Smith (9) summarized studies on the uptake of P by mycorrhizae and suggested that phosphate uptake by the host tissue is through the fungal symbiont. There appear to be at least two pools of phosphate within the mycorrhizal structure, a small pool of phosphate that is readily translocated from the fungus to the host, and a larger, accumulated pool of relatively inactive, inorganic phosphate. Light microscopic studies have shown the presence of inorganic phosphate as metachromatic staining granules within the fungal '

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confidence: 78%

Phosphorus Distribution in Red Pine Roots and the Ectomycorrhizal Fungus Hebeloma arenosa

MacFall¹,

Slack²,

Wehrli³

1992

Plant Physiol.

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“…A concentration step is frequently required after a clarified solution of protein has been obtained, in order to aid subsequent purification steps (1,2). This can be done by a variety of procedures such as ultrafiltration or in vacuo (1).…”

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confidence: 99%

“…Detailed procedures for estimating quantities of different Pcontaining compounds are available for fungi (1) and phytoplankton (2). However, these methods are time consuming and provide a degree of detail unnecessary for many purposes.…”

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confidence: 99%

“…One of the simplest and quickest methods of concentrating solutions of proteins is to add inert matrix polymer, such as Sephadex, to the protein solution, which allows the water and other small molecules to be absorbed (1,2). Besides, for small-scale applications solution can be concentrated by dialysis against polyethylene glycol (PEG) 1 or dry Sephadex (1-3). In these techniques the protein solution to be…”

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confidence: 99%

“…The fate of phosphorus (P) 1 within macroalgae and allocation of P to different structural components are important aspects of algal nutrition studies. Detailed procedures for estimating quantities of different Pcontaining compounds are available for fungi (1) and phytoplankton (2).…”

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confidence: 99%

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