Characterization of the cytokine and maturation responses of pure populations of porcine plasmacytoid dendritic cells to porcine viruses and toll-like receptor agonists

Calzada-Nova, Gabriela; Schnitzlein, William M.; Husmann, Robert J.; Zuckermann, Federico A.

doi:10.1016/j.vetimm.2009.10.026

Cited by 42 publications

(58 citation statements)

References 69 publications

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“…Independent of this issue requiring further clarification, pDC are more likely to promote Th1 responses through secretion of type I IFNs, IL-12 and TNF-α [2]. We and others have also found this profile of cytokines for porcine pDC [1], [34].…”

Section: Discussionmentioning

confidence: 55%

Regulation of Porcine Plasmacytoid Dendritic Cells by Cytokines

Lannes¹,

Summerfield²

2013

PLoS ONE

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Plasmacytoid dendritic cells (pDC) are the most potent producers of type-I interferon (IFN) and represent the main interferon (IFN)-α source in response to many viruses. Considering the important roles played by type I IFN’s, not only as antiviral effectors but also as potent alarming cytokine of the immune system, we investigated how such responses are regulated by various cytokines. To this end, we stimulated enriched pDC in the presence or absence of particular cytokines with a strong activator, CpG DNA, or a weak activator of pDC, foot-and-mouth disease virus (FMDV). Alternatively, we pre-incubated pDC for 16 h before stimulation. The pro-inflammatory cytokines tested Interleukin (IL)-6, IL17A, tumour necrosis factor (TNF)-α did not influence IFN-α responses except TNF-α, which promoted responses induced by FMDV. The haematopoietic cytokines Fms-related tyrosine kinase 3 ligand (Flt3-L) and granulocyte-macrophage colony-stimulating factor (GM-CSF) had enhancing effects on pDC activation at least in one of the protocols tested. IFN-β and IFN-γ were the most potent at enhancing FMDV-induced IFN-α, up to 10-fold. Interestingly, also the Th2 cytokine IL-4 was an efficient promoter of pDC activity, while IL-10 was the only negative regulator of IFN-α in pDC identified. The cytokines enhancing IFN-α responses also promoted pDC survival in cell culture with the exception of GM-CSF. Taken together this work illustrates how the cytokine network can influence pDC activation, a knowledge of relevance for improving vaccines and therapeutic interventions during virus infections, cancers and autoimmune diseases in which pDC play a role.

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Section: Discussionmentioning

confidence: 55%

Regulation of Porcine Plasmacytoid Dendritic Cells by Cytokines

Lannes¹,

Summerfield²

2013

PLoS ONE

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“…TLR2), poly(I:C) (?TLR3) (Auray et al, 2010), lipopolysaccharide (LPS ? TLR4) Uddin et al, 2012), imiquimod (?TLR7/8) (Calzada-Nova et al, 2010), CpG ODN (?TLR9) (Auray et al, 2010;Calzada-Nova et al, 2010;Dar et al, 2010), and muramil dipeptide (MDP ? NOD) (Jozaki et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…In contrast to humans, imiquimod could not only activate TLR7 but also TLR8 in the pig, showing altered receptor specificity across species (Zhu et al, 2008). Calzada-Nova et al found an enhanced production of various cytokines (IFN-a, IFN-c, IL-2, IL-6, IL-8, IL-12, and TNF-a) after plasmacytoid DCs (pDCs) were stimulated with both imiquimod and the TLR9 ligand CpG ODN (Calzada-Nova et al, 2010). CpG ODN additionally increased the expression of chemokine receptors on monocyte derived DCs (MoDCs) and blood DCs (bDCs) (Auray et al, 2010) as well as altering the general DC morphology of cultured sorted plasmacytoid (pDCs) compared to unstimulated cultures (Calzada-Nova et al, 2010).…”

Section: Toll-like Receptorsmentioning

confidence: 99%

“…Calzada-Nova et al found an enhanced production of various cytokines (IFN-a, IFN-c, IL-2, IL-6, IL-8, IL-12, and TNF-a) after plasmacytoid DCs (pDCs) were stimulated with both imiquimod and the TLR9 ligand CpG ODN (Calzada-Nova et al, 2010). CpG ODN additionally increased the expression of chemokine receptors on monocyte derived DCs (MoDCs) and blood DCs (bDCs) (Auray et al, 2010) as well as altering the general DC morphology of cultured sorted plasmacytoid (pDCs) compared to unstimulated cultures (Calzada-Nova et al, 2010). A more detailed study including different CpG ODNs revealed that the three classes of CpG ODN (A-C) induce different cytokines (Dar et al, 2010).…”

Section: Toll-like Receptorsmentioning

confidence: 99%

See 1 more Smart Citation

The porcine innate immune system: An update

Mair

Sedlak

Käser

et al. 2014

Developmental & Comparative Immunology

205

157

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Over the last few years, we have seen an increasing interest and demand for pigs in biomedical research. Domestic pigs (Sus scrofa domesticus) are closely related to humans in terms of their anatomy, genetics, and physiology, and often are the model of choice for the assessment of novel vaccines and therapeutics in a preclinical stage. However, the pig as a model has much more to offer, and can serve as a model for many biomedical applications including aging research, medical imaging, and pharmaceutical studies to name a few. In this review, we will provide an overview of the innate immune system in pigs, describe its anatomical and physiological key features, and discuss the key players involved. In particular, we compare the porcine innate immune system to that of humans, and emphasize on the importance of the pig as model for human disease.

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“…Since PRRSV can infect DCs (Loving et al, 2007), the response of DCs to PRRSV infection is of interest. The production of type I IFNs by pDCs is severely suppressed during PRRSV infection (Baumann et al, 2013;Calzada-Nova et al, 2010). Furthermore, the pDCs activation, which is featured by the increase of CD80/86 expression and cell-type development, is not seen by PRRSV (Calzada-Nova et al, 2011).…”

Section: Porcine DC Response To Prrsv Infectionmentioning

confidence: 98%

The viral innate immune antagonism and an alternative vaccine design for PRRS virus

Yoo

2017

Veterinary Microbiology

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Porcine reproductive and respiratory syndrome (PRRS) remains one of the most economically significant diseases in the swine industry worldwide. The current vaccines are less satisfactory to confer protections from heterologous infections and long-term persistence, and the need for better vaccines are urgent. The immunological hallmarks in PRRSV-infected pigs include the unusually poor production of type I interferons (IFNs-α/β) and the aberrant and delayed adaptive immune responses, indicating that PRRSV has the ability to suppress both innate and adaptive immune responses in the host. Type I IFNs are the potent antiviral cytokines and recent studies reveal their pleiotropic functions in the priming of expansion and maturation of adaptive immunity. Thus, IFN antagonism-negative PRRSV is hypothesized to be attenuated and to build effective and broad- spectrum innate and adaptive immune responses in pigs. Such vaccines are promising alternatives to traditional vaccines for PRRSV.

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Characterization of the cytokine and maturation responses of pure populations of porcine plasmacytoid dendritic cells to porcine viruses and toll-like receptor agonists

Cited by 42 publications

References 69 publications

Regulation of Porcine Plasmacytoid Dendritic Cells by Cytokines

Regulation of Porcine Plasmacytoid Dendritic Cells by Cytokines

The porcine innate immune system: An update

The viral innate immune antagonism and an alternative vaccine design for PRRS virus

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