Characterization of the compound of longan honey from indonesia using LC-MS/MS and FTIR and the mechanism of inhibition of HEp-2 cells

Abstract

Indonesian honey contains active compounds that have the potential as antioxidant and anticancer, especially as anticancer of the larynx through inhibition of HEp-2 cells. This study aims to determine the active compounds in longan honey and proposes the mechanism of action in inhibiting HEp-2 cells. The sample was used in the form of longan obtained from honey bee breeders in Central Java. Honey samples were extracted using methanol, and then liquid-liquid partitioning was carried out successively using n-hexane and ethyl acetate. Isolation and characterization of longan honey samples using FTIR and LC-MS/MS showed the presence of the following compounds: Xanthoxol glycosides, Santonin, Octadecanamide, Indole-3 carboxylaldehyde, 3,4-dimethoxycinnamic acid, Dimethyl esculetin, Tryptophan, O-acetyl-L-serine, D-glucose-6-phosphate, Feruloiltyramine, Lauryl diethanolamide, Taurine, 6-mercaptopurine, 3-(2,4-dichlorophenyl)-4-phenylcoumarin, 3',4'-dimethoxy-3-hydroxy-6methylflavone and D-1-((3-carboxypropyl)amino)-1-deoxyfructose. The compounds in longan honey against HEp-2 cells is quercetin, 3,6-dimethoxycinnamic acid, phenyl coumarin, dimethyl esculetin, santonin, 6-mercaptopurine, and feruloyltyramine. The proposed mechanism of active compound in honey to inhibit HEp-2 cells in several ways including via caspase pathway and purine synthesis and other relevant mechanisms. However, this assumption needs to be tested further to obtain more precise information regarding the mechanism of inhibition of HEp-2 cells.